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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,632 results for: t7

Showing: 2061 - 2080 of 10632 results

  1. 5C8H

    Plasmid
    #69969
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ORC2:JMC095-D11:C231601 (ORC2 Human)
    Tags
    His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    Oct. 7, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  2. TBXTA-c019

    Plasmid
    #139756
    Purpose
    For protein expression
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, WT (TBXT Human)
    Tags
    His6-SUMO
    Expression
    Bacterial
    Mutation
    Contains only amino acids E41-A214
    Promoter
    T7
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139756-plasmid-map-sequence-id-296514

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  3. pMSP1D1Q1

    Plasmid
    #97326
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional Q residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Michael Marty
    Insert
    MSP1D1Q1
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of Q residue after T25
    Promoter
    T7
    Available Since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97326-plasmid-map-sequence-id-182622

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  4. TBXTA-c020

    Plasmid
    #139757
    Purpose
    For protein expression/crystallography (PDB 5QS0)
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, WT (TBXT Human)
    Tags
    His6-SUMO
    Expression
    Bacterial
    Mutation
    Contains only amino acids E41-N211
    Promoter
    T7
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139757-plasmid-map-sequence-id-296518

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  5. 4Z30

    Plasmid
    #69957
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RC3H2:YTC020-E12:C234063 (RC3H2 Human)
    Tags
    His
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    Oct. 7, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. 4JXT A

    Plasmid
    #60105
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RPRD1A:DCC020-G05:C222609 (RPRD2 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. 4LD6

    Plasmid
    #60088
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PWWP2B:JMC079-A09:C228600 (PWWP2B Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. pBSII-LA-mCherry(AM)

    Plasmid
    #244815
    Purpose
    In-Vitro Synthesis of mRNA encoding a Honeybee-optimized mCherry-labeled Lifeact (LA) Tag
    Depositor
    Frederic Strobl
    Insert
    mCherry
    Use
    In-vitro mrna synthesis
    Tags
    Lifeact
    Mutation
    coding sequence is optimized for the Honeybee
    Promoter
    T7
    Available Since
    Oct. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    244815-plasmid-map-sequence-id-489770

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  9. pBSII-MEME-mTagBFP(AM)

    Plasmid
    #244810
    Purpose
    In-Vitro Synthesis of mRNA encoding a Honeybee-optimized mTagBFP2-labeled GAP43 Membrane Anchor (MEME) Tag
    Depositor
    Frederic Strobl
    Insert
    mTagBFP2
    Use
    In-vitro mrna synthesis
    Tags
    GAP43 Membrane Anchor (MEME) Tag
    Mutation
    coding sequence is optimized for the Honeybee
    Promoter
    T7
    Available Since
    Oct. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    244810-plasmid-map-sequence-id-489768

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  10. pBSII-NLS-mCherry(AM)

    Plasmid
    #244814
    Purpose
    In-Vitro Synthesis of mRNA encoding a Honeybee-optimized mCherry-labeled Nuclear Localization Signal (NLS) Tag
    Depositor
    Frederic Strobl
    Insert
    mCherry
    Use
    In-vitro mrna synthesis
    Tags
    SV40 NLS
    Mutation
    coding sequence is optimized for the Honeybee
    Promoter
    T7
    Available Since
    Oct. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    244814-plasmid-map-sequence-id-489766

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  11. pBSII-MEME-mEmerald(AM)

    Plasmid
    #244813
    Purpose
    In-Vitro Synthesis of mRNA encoding a Honeybee-optimized mEmerald-labeled GAP43 Membrane Anchor (MEME) Tag
    Depositor
    Frederic Strobl
    Insert
    mEmerald
    Use
    In-vitro mrna synthesis
    Tags
    GAP43 Membrane Anchor (MEME) Tag
    Mutation
    coding sequence is optimized for the Honeybee
    Promoter
    T7
    Available Since
    Oct. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    244813-plasmid-map-sequence-id-489762

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  12. pBSII-MEME-mCherry(AM)

    Plasmid
    #244816
    Purpose
    In-Vitro Synthesis of mRNA encoding a Honeybee-optimized mCherry-labeled GAP43 Membrane Anchor (MEME) Tag
    Depositor
    Frederic Strobl
    Insert
    mCherry
    Use
    In-vitro mrna synthesis
    Tags
    GAP43 Membrane Anchor (MEME) Tag
    Mutation
    coding sequence is optimized for the Honeybee
    Promoter
    T7
    Available Since
    Oct. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    244816-plasmid-map-sequence-id-489761

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  14. CPH3737_GST-TEV-Spt6p-tSH2-1223-1453

    Plasmid
    #227087
    Purpose
    Bacterial expression of K1355A/K1435A double-mutant GST-tagged Spt6 tSH2 domain (1223-1453)
    Depositor
    Chris Hill
    Insert
    spt6-tsh2 (SPT6 Budding Yeast)
    Tags
    GST-TEV_cleavage_site
    Expression
    Bacterial
    Mutation
    K1355A, K1435A
    Promoter
    T7
    Available Since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    227087-plasmid-map-sequence-id-470399

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  15. pAIP4_1T3Y

    Plasmid
    #234228
    Purpose
    Heterologous protein expression of coactosin-like protein in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1T3Y (COTL1 Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234228-plasmid-map-sequence-id-464193

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  16. pAIP4_1JL1

    Plasmid
    #234206
    Purpose
    Heterologous protein expression of ribonuclease HI (RNase HI) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1JL1 (rnhA Escherichia coli)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234206-plasmid-map-sequence-id-464171

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  17. pAIP4_3TOW

    Plasmid
    #234201
    Purpose
    Heterologous protein expression of multivesicular body subunit 12B (ESCRT-I complex subunit MVB12B) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3TOW (MVB12B Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234201-plasmid-map-sequence-id-464166

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  18. pAIP4_1KQX

    Plasmid
    #234181
    Purpose
    Heterologous protein expression of cellular retinol-binding protein type II in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1KQX (rbp2a Zebrafish)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234181-plasmid-map-sequence-id-464146

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  19. pAIP4_1AKO

    Plasmid
    #234162
    Purpose
    Heterologous protein expression of exodeoxyribonuclease III (Exonuclease III) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1AKO (xthA Escherichia coli K-12)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234162-plasmid-map-sequence-id-464128

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  20. pAIP4_4NFB

    Plasmid
    #234161
    Purpose
    Heterologous protein expression of paired immunoglobulin-like type 2 receptor alpha in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4NFB (PILRA Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234161-plasmid-map-sequence-id-464127

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Showing: 2061 - 2080 of 10632 results