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pT7-EGFP-C1-HsDCP2-W44A_V

Plasmid

#147816

Purpose

Mammalian Expression of HsDCP2-W44A

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP2-W44A (DCP2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP2_1-244_V

Plasmid

#147807

Purpose

Mammalian Expression of HsDCP2_1-244

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP2_1-244 (DCP2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP2_97-392_V

Plasmid

#147809

Purpose

Mammalian Expression of HsDCP2_97-392

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP2_97-392 (DCP2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 BS (-) N protein

Plasmid

#188448

Purpose

SARS CoV-2 N protein expression

Depositor

Ajit Chande

Article

Mishra et al Life Sci Alliance. 2022 Mar 16;5(7). pii

Insert

SARS-CoV-2 N protein

Tags

Strep-Tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pJ414-PCY1:S495A

Plasmid

#98151

Purpose

Expresses PCY1:S495A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1:S495A

Tags

His6-TEV

Expression

Bacterial

Mutation

Changed serine 495 to alanine

Promoter

T7

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pJ414-PCY1:W603A

Plasmid

#98153

Purpose

Expresses PCY1:W603A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1:W603A

Tags

His6-TEV

Expression

Bacterial

Mutation

Changed tryptophan 603 to alanine

Promoter

T7

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pJ414-PCY1:H695Q

Plasmid

#98154

Purpose

Expresses PCY1:H695Q from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1:H695Q

Tags

His6-TEV

Expression

Bacterial

Mutation

Changed histidine 695 to glutamine

Promoter

T7

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pJ414-PCY1:H695A

Plasmid

#98155

Purpose

Expresses PCY1H695A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1:H695A

Tags

His6-TEV

Expression

Bacterial

Mutation

Changed histidine 695 to alanine

Promoter

T7

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pJ414-PCY1:Y696G

Plasmid

#98156

Purpose

Expresses PCY1:R696G from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1:Y696G

Tags

His6-TEV

Expression

Bacterial

Mutation

Changed tyrosine 696 to glycine

Promoter

T7

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-RanGAP*

Plasmid

#175237

Purpose

Bacterial expression and purification, low affinity SUMOylation substrate, point mutation F562A reduces affinity for E2, increasing the Km

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

RANGAP1 (RANGAP1 Human)

Expression

Bacterial

Mutation

Amino acid 562 F to A

Promoter

tac

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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