We narrowed to 4,090 results for: PRS

1. pRS406 ATG1-ATG7-3'UTR

   Plasmid

   #166847

   Purpose

   Expresses Atg1 with ATG7 3'UTR in yeasts.

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Mol Cell. 2019 Jan 17;73(2):314-324.e4.

   Insert

   ATG1 (ATG1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ATG1 Promoter

   Available since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS405 SEC66-VAC8-GFP

   Plasmid

   #166840

   Purpose

   Expresses ER localized Vac8-GFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

   Insert

   VAC8 (VAC8, SEC66 Budding Yeast)
   

   Use

   Tags

   GFP and SEC66 transmembrane domain

   Expression

   Yeast

   Mutation

   SEC66 transmembrane domain

   Promoter

   VAC8 promoter

   Available since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS405 VAC8-G2A-GFP

   Plasmid

   #166837

   Purpose

   Expresses a myristoylation mutant Vac8-GFP in yeasts, mutation is Gly2Ala

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

   Insert

   VAC8 (VAC8 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   G2A

   Promoter

   VAC8 promoter

   Available since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSFDuet1-tglA(C51)HIB

   Plasmid

   #134675

   Purpose

   Expresses His6-TglA(C51), TglH, TglI and TglB in E. Coli

   Depositor

   Wilfred van der Donk

   Article

   Ting et al Science. 2019 Jul 19;365(6450):280-284.

   Inserts

   TglA(C51)
   TglH
   TglI
   TglB
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   insertion of cysteine 51

   Promoter

   T7

   Available since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRS416-PTEF1-MCP-3P-ECM33_intron

   Plasmid

   #127601

   Purpose

   low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 3P-ECM33 intron inserted into URA3

   Depositor

   David Bartel

   Article

   Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

   Insert

   ECM33 intron with MS2 hairpins inserted near 3′ end of intron
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS416-PTEF1-MCP-5M-ECM33_intron

   Plasmid

   #127600

   Purpose

   low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5M-ECM33 intron inserted into URA3

   Depositor

   David Bartel

   Article

   Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

   Insert

   ECM33 intron with MS2 hairpins replacing sequence near 5′ end of intron
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRS416-PTEF1-MCP-5P-ECM33_intron

   Plasmid

   #127599

   Purpose

   low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5P-ECM33 intron inserted into URA3

   Depositor

   David Bartel

   Article

   Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

   Insert

   ECM33 intron with MS2 hairpins inserted near 5′ end of intron
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS416-PTEF1-MCP-ECM33_intron

   Plasmid

   #127598

   Purpose

   low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, ECM33 intron inserted into URA3

   Depositor

   David Bartel

   Article

   Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

   Insert

   ECM33 intron
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS416-PTEF1-MCP-no_intron

   Plasmid

   #127597

   Purpose

   low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter

   Depositor

   David Bartel

   Article

   Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

   Insert

   1xFLAG-MCP expression cassette
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRS-ATG1(mut)(406)

    Plasmid

    #122069

    Purpose

    Expressing a mutant ATG1

    Depositor

    Daniel Klionsky

    Article

    Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

    Insert

    ATG1 (ATG1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Codon changes without changing the amino acid seq…

    Promoter

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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