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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,752 results for: golden gate

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Showing: 1051 - 1060 of 4752 results

  1. SP_A4

    Plasmid
    #228148
    Purpose
    Plasmid contains the secretion signal peptide sequence of the human CHADL gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
    Depositor
    Joseph Brock
    Article
    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86
    Insert
    sp_Q6NUI6_CHADL_HUMAN:0-30
    Use
    Synthetic Biology
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228148-plasmid-map-sequence-id-451693

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  2. SP_A5

    Plasmid
    #228149
    Purpose
    Plasmid contains the secretion signal peptide sequence of the human GDF8 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
    Depositor
    Joseph Brock
    Article
    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86
    Insert
    sp_O14793_GDF8_HUMAN:0-18
    Use
    Synthetic Biology
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228149-plasmid-map-sequence-id-451779

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  3. pKEK3236

    Plasmid
    #234108
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    sfCherry2
    Expression
    Bacterial
    Mutation
    E117Q/T127I/G219A
    Promoter
    pJ23100-BsaI
    Available Since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    234108-plasmid-map-sequence-id-467997

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  4. pKEK3221

    Plasmid
    #234100
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    sfcherry 2
    Expression
    Bacterial
    Mutation
    E117Q/T127I/G219A
    Promoter
    pJ23100-BsaI
    Available Since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    234100-plasmid-map-sequence-id-468004

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  5. pKEK3237

    Plasmid
    #234091
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    sfGFP
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    S30R/Y39N/N105T/Y145F/I171V/A206V
    Promoter
    pJ23100-BsaI
    Available Since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    234091-plasmid-map-sequence-id-467987

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  6. pKEK3397

    Plasmid
    #234081
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    FuGFP
    Expression
    Bacterial
    Promoter
    pJ23100-BsaI
    Available Since
    April 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    234081-plasmid-map-sequence-id-468005

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  7. pKEK3224

    Plasmid
    #234086
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    FuGFP
    Expression
    Bacterial
    Promoter
    pJ23100-BsaI
    Available Since
    April 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    234086-plasmid-map-sequence-id-468001

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  8. pKEK3197

    Plasmid
    #234095
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    YukonOFP
    Expression
    Bacterial
    Promoter
    pJ23100-BsaI
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234095-plasmid-map-sequence-id-464928

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  9. pKEK3199

    Plasmid
    #234097
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    tspurple
    Expression
    Bacterial
    Promoter
    pJ23100-BsaI
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234097-plasmid-map-sequence-id-464933

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  10. pKEK3200

    Plasmid
    #234098
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    fuGFP
    Expression
    Bacterial
    Promoter
    pJ23100-BsaI
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234098-plasmid-map-sequence-id-464935

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Showing: 1051 - 1060 of 4752 results