We narrowed to 148,810 results for: ins;

1. pRetroX-TRE3G-SopE

   Plasmid

   #221327

   Purpose

   Dox-inducible expression of SopE in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   SopE
   

   Use

   Retroviral

   Tags

   FLAG

   Promoter

   TRE3GV

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS FGF3 isoform 1

   Plasmid

   #19230

   Depositor

   Cliff Tabin

   Article

   Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

   Insert

   Fibroblast growth factor receptor 3 isoform 1 (FGFR3 Chicken)
   

   Expression

   Bacterial

   Available Since

   Sept. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pCMV-T7-ABE8e-nSpCas9-NG-P2A-EGFP (KAC999)

   Plasmid

   #185914

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8e-nSpCas9-NG-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…

   Promoter

   CMV and T7

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-ABE8.20m-nSpCas9-NG-P2A-EGFP (KAC1164)

   Plasmid

   #185919

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8.20m-nSpCas9-NG-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…

   Promoter

   CMV and T7

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFX-Tom20-EGFP

   Plasmid

   #174186

   Purpose

   Express EGFP with mitochondria-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   EGFP with mitchondria-targeting sequence
   

   Tags

   the mitochondria-targeting sequence of TOM20

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP-alphasynuclein-A53T

   Plasmid

   #40823

   Depositor

   David Rubinsztein

   Article

   Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

   Insert

   SNCA (SNCA Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   A53T (aggregates more rapidly than the wild-type …

   Promoter

   CMV

   Available Since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSFFV_sfCherry3C(1-10)

   Plasmid

   #117482

   Purpose

   Lentiviral vector for constitutive expression of sfCherry3C(1-10)

   Depositor

   Bo Huang

   Article

   Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

   Insert

   sfCherry3C(1-10)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   5 substitutions (K45R, G52D, T106A, K182R, N194D)…

   Promoter

   SFFV

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMJA089

   Plasmid

   #128518

   Purpose

   Expresses human Lysozyme in Pichia pastoris

   Depositor

   Marcos Alcocer

   Article

   Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

   Insert

   Artificial gene for human lysozyme (LYZ Human)
   

   Expression

   Yeast

   Promoter

   AOX1

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309)

   Plasmid

   #185913

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

   Promoter

   CMV and T7

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. YFVdelCME-mVenus

    Plasmid

    #179953

    Purpose

    pCCI vector encoding YFV-17D lacking C, prM, E proteins but with mVenus

    Depositor

    Wei Xu

    Article

    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

    Insert

    YFV-17D lacking C, prM, E proteins but with mVenus
    

    Use

    Transneuronal tracing

    Promoter

    SP6

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330-EN1201

    Plasmid

    #92144

    Purpose

    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locus

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    spCas9-nuclease and sgRNA against mouse TIGRE acceptor locus
    

    Expression

    Mammalian

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. DT960

    Plasmid

    #80412

    Purpose

    Expresses 960 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

    Depositor

    Thomas Cooper

    Article

    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

    Insert

    human DMPK exons 11-15 with 960 interrupted CTG repeats (DMPK Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFX-Tom20-ｍCherry

    Plasmid

    #174188

    Purpose

    Express mCherry with mitochondria-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    mCherry with mitchondria-targeting sequence
    

    Tags

    the mitochondria-targeting sequence of TOM20

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAcGFP1-mito

    Plasmid

    #174180

    Purpose

    Express AcGFP1 with mitochondria-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    AcGFP with mitochondria-targeting sequence
    

    Tags

    the mitochondria-targeting sequence of the cytoch…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-TRPV1exCellHalo

    Plasmid

    #220307

    Purpose

    Mammalian expression vector for a subunit of rat TRPV1 with circularly permutated HaloTag inserted between the S5 and S6 transmembrane helices

    Depositor

    Eric Senning

    Article

    Mott et al J Gen Physiol. 2024 Oct 7;156(10):e20231

    Insert

    rTRPV1exCellHalo (Trpv1 Rat)
    

    Tags

    circularly permutated Halotag (Deo et al., Nat Ch…

    Expression

    Mammalian

    Mutation

    linker included to insert cpHaloTag (

    Promoter

    CMV promoter

    Available Since

    July 30, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. Venus-BclXL-pEGFP-C1

    Plasmid

    #177409

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bcl-XL.

    Depositor

    David Andrews

    Article

    Aranovich et al Mol Cell. 2012 Mar 30;45(6):754-63. doi:

    Insert

    Bcl-XL
    

    Tags

    Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBS-KS-attB1-2-PT-SA-SD-1-GFP11x7

    Plasmid

    #172067

    Purpose

    Protein trap plasmid for inserting GFP11x7 into a phase 1 coding intron

    Depositor

    Daichi Kamiyama

    Article

    Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    GFP11x7
    

    Expression

    Insect

    Available Since

    Oct. 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pETM6-RgTALsyn

    Plasmid

    #100947

    Purpose

    Expression of Tyrosine Ammonia Lyase from inducible T7 promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

    Insert

    RgTALsyn
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Silent mutation to remove internal NdeI site on T…

    Promoter

    T7

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21 MBPx-SpyCatcher

    Plasmid

    #72327

    Purpose

    Bacterial expression of tandem high affinity maltose binding protein domains linked to SpyCatcher, for solid-phase polyprotein synthesis.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    pET21 MBPx-SpyCatcher
    

    Tags

    N-terminal His6 tag

    Expression

    Bacterial

    Mutation

    MBP contains A312V, I317V mutations and 172, 173,…

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSFFV_sfCherry3V(1-10)

    Plasmid

    #117483

    Purpose

    Lentiviral vector for constitutive expression of sfCherry3V(1-10)

    Depositor

    Bo Huang

    Article

    Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

    Insert

    sfCherry3V(1-10)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    6 substitutions (K45R, G52D, L83W, T106A, K182R, …

    Promoter

    SFFV

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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