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pcDNA-Cas9-T2A-STOP-TdT

Plasmid

#126425

Purpose

Expresses Cas9 but not TdT in mammalian cells; control for pcDNA-Cas9-T2A-TdT. (pTBL552)

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

Cas9-T2A-STOP-TdT (DNTT Streptococcus pyogenes, Human)

Expression

Mammalian

Mutation

alanine-6 of TdT has been changed to a STOP codon

Promoter

pCMV

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_B2M_WT

Plasmid

#81810

Purpose

Gateway Donor vector containing B2M , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

B2M (B2M Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAP3K14_WT

Plasmid

#82322

Purpose

Gateway Donor vector containing MAP3K14 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAP3K14 (MAP3K14 Human)

Use

Gateway entry vector

Mutation

L353P

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KEAP1_WT

Plasmid

#82077

Purpose

Gateway Donor vector containing KEAP1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KEAP1 (KEAP1 Human)

Use

Gateway entry vector

Mutation

C624S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ILKAP_WT

Plasmid

#81809

Purpose

Gateway Donor vector containing ILKAP , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ILKAP (ILKAP Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pKHH030_sgRB1#1

Plasmid

#174144

Purpose

Lentiviral vector expressing a sgRNA against the human RB1 gene

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

RB1 (RB1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_NRAS_p.Q61H

Plasmid

#81670

Purpose

Gateway Donor vector containing NRAS, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NRAS (NRAS Human)

Use

Gateway entry vector

Mutation

Q61H

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RHOA_WT

Plasmid

#81805

Purpose

Gateway Donor vector containing RHOA , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RHOA (RHOA Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3R4_WT

Plasmid

#81859

Purpose

Gateway Donor vector containing PIK3R4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3R4 (PIK3R4 Human)

Use

Gateway entry vector

Mutation

T231R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_GALNT14_p.V534F

Plasmid

#81468

Purpose

Gateway Donor vector containing GALNT14 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

GALNT14 (GALNT14 Human)

Use

Gateway entry vector

Mutation

L4V; V534F

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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