We narrowed to 13,583 results for: TIM

1. pMIII-Neo-BFP

   Plasmid

   #153426

   Purpose

   Retroviral (MSCV) expression of mTagBFP2 fluorescent protein

   Depositor

   Maki Nakayama

   Article

   Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

   Insert

   mTagBFP2
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pcDNA3.2/Capsid

   Plasmid

   #120816

   Purpose

   Ectopic expression of ZIKV protein

   Depositor

   Jan Rehwinkel

   Article

   Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

   Insert

   Capsid ZIKV
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-MSP1D1delltaH4

   Plasmid

   #71713

   Purpose

   Membrane scaffold protein (MSP), helix 4 deletion

   Depositor

   Gerhard Wagner

   Article

   Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

   Insert

   Apolipoprotein A1 (APOA1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-HiBiT-CD63

   Plasmid

   #162591

   Purpose

   N-terminally HiBiT-tagged human CD63 under CAG promoter

   Depositor

   Masaharu Somiya

   Article

   Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

   Insert

   human CD63 (CD63 Human, human, Synthetic)
   

   Use

   Tags

   HiBiT

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMOS012: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

   Plasmid

   #163057

   Purpose

   Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   Grx1-roGFP1-iEER
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKT-iRFP-KAN

   Plasmid

   #64687

   Purpose

   pKT vector encoding yeast-codon optimized infrared fluorescent protein iRFP.

   Depositor

   Erin O'Shea

   Article

   Hansen et al Mol Syst Biol. 2013 Nov 5;9:704. doi: 10

   Insert

   iRFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Nucleotide sequence has been yeast-codon optimize…

   Promoter

   Available since

   May 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBITE-EGFRvIII-F271-OKT3

   Plasmid

   #190682

   Purpose

   Encodes an EGFRvIII-CD3 BITE protein (F271 clone)

   Depositor

   Scott McComb

   Article

   Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

   Insert

   Anti-EGFRvIII-scFv-F271-modularized-OKT3-HisTag
   

   Use

   Lentiviral

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA5/FRT/TO_CalR-HaloTag7-P30-SNAP-tag-KDEL

   Plasmid

   #182012

   Purpose

   CMV overexpression of HaloTag7 and SNAP-tag fusion in the endoplasmic reticulum of mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

   Insert

   CalR-HaloTag7-P30-SNAP-tag-KDEL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCG-TPMV Hd32-IL-13

   Plasmid

   #163869

   Purpose

   Expresses TPMV H glycoprotein bearing IL-13 in mammalian cells

   Depositor

   Jakob Reiser

   Article

   Argaw et al Mol Ther Methods Clin Dev. 2021 Apr 24;2

   Insert

   TPMV H glycoprotein/IL-13
   

   Use

   Tags

   Human IL-13

   Expression

   Mammalian

   Mutation

   Amino acids 2 to 33 at N terminus deleted

   Promoter

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. TagBFP2-C1-SACM1LdeltaTMD-C389S-Fis1tail

    Plasmid

    #220079

    Purpose

    SAC1 with a C389S mutation in it with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Blue Fluorophore

    Depositor

    Gerry Hammond

    Article

    Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

    Insert

    SACM1l-C389S-Fis1tail (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C389SdeltaTMD

    Promoter

    Available since

    May 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZR043_Lenti-U6-sgEGFR-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

    Plasmid

    #180267

    Purpose

    Lentiviral expression vector for CRISPRa-SAM with EGFR targeting sgRNA

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    EGFR sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC18T-mini-Tn7T-Gm-Pc-mTurquoise2

    Plasmid

    #111259

    Purpose

    mini-Tn7 vector for insertion of mTurquoise2 (codon optimized for P. fluorescens) at bacterial attTn7 site

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mTurquoise2 is codon optimized for expression in …

    Promoter

    Pc

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.2/Envelope

    Plasmid

    #120821

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    Envelope ZIKV
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. G10 Cas9 MDC123

    Plasmid

    #59187

    Purpose

    G10 promoting Glycing Max codon optimized Cas9 (N and C terminus NLS) with bar plant selection

    Depositor

    Robert Stupar

    Article

    Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

    Insert

    Glycine Max codon optimized Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    G10

    Available since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPomyces-1

    Plasmid

    #61736

    Purpose

    Streptomyces expression of codon-optimized Cas9, tracrRNA, and custom crRNA

    Depositor

    Huimin Zhao

    Article

    Cobb et al ACS Synth Biol. 2014 Dec 8.

    Inserts

    sSpCas9
    tracrRNA
    crRNA cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    BbsI-flanked lacZ cassette inserted in place of s…

    Promoter

    gapdhp(EL), rpsLp(CF), and rpsLp(XC)-BbsI

    Available since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDIV300

    Plasmid

    #204968

    Purpose

    AMA1 plasmid with Aspergillus optimized Mad7 and hph (hygromycin) resistance marker

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

    Inserts

    Mad7
    hph (hygromycin resistance marker)
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans tef1 promoter and Aspergillu…

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGLucIPZ

    Plasmid

    #228703

    Purpose

    Constitutive expression of Gaussia luciferase, puromycin N-acetyl-transferase and shRNAmir of interest

    Depositor

    Thorsten Stiewe

    Article

    Charles et al Nat Commun. 2014 Jun 3;5:3981. doi: 10.1

    Inserts

    Gaussia luciferase
    non-silencing shRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pFD115

    Plasmid

    #124768

    Purpose

    pFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector

    Depositor

    David Bikard

    Article

    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

    Inserts

    Ptet
    cas9
    term PpflB sgRNA (BsaI)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ptet

    Available since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETM6-G6-vioABECD

    Plasmid

    #66536

    Purpose

    pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with G6 promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Inserts

    VioA
    VioB
    VioE
    VioC
    VioD
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Mutant T7 Promoter - G6

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. Twitch-1 pcDNA3

    Plasmid

    #49812

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Insert

    Twitch-1
    

    Use

    Tags

    ECFP and cpCit174

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

21. pF208wt-luc

    Plasmid

    #122143

    Purpose

    For luciferase expression

    Depositor

    Carmen Williams

    Article

    Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. d

    Insert

    Fam208a promoter (Tasor Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Fam208a

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSJ1458 (pFA6-link-mTurquoise2-CaURA3MX)

    Plasmid

    #86424

    Purpose

    PCR tagging module with yeast codon optimized mTurquoise2

    Depositor

    Sue Jaspersen

    Article

    Burns et al Elife. 2015 Sep 15;4. doi: 10.7554/eLife

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUC-hU6-crScaffold_SV40-BFP

    Plasmid

    #224860

    Purpose

    crRNA scaffold for RfxCas13d expressed from hU6 promoter and reporter BFP protein expressed from SV40 promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. ptagRFP-MS2coatProtein

    Plasmid

    #103831

    Purpose

    Live cell reader for MS2 loop RNA production

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    MS2 coat protein (MCP) (cp )
    

    Use

    Tags

    tagRFP

    Expression

    Mammalian

    Mutation

    MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

    Promoter

    CMV

    Available since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PKG I alpha CAT

    Plasmid

    #16392

    Depositor

    Bernard Weinstein

    Article

    Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

    Insert

    PKG1 alpha (PRKG1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    CAT: catalytic domain, S65D

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

26. pMOS013E: Entry vector for: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

    Plasmid

    #163073

    Purpose

    Entry vector for: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    mito-roGFP2-Grx1
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCNL-N1_vinculin

    Plasmid

    #65691

    Purpose

    Expresses Vinculin-CNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    vinculin (Vcl Mouse)
    

    Use

    Luciferase

    Tags

    CNL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

28. pJH4542

    Plasmid

    #200779

    Purpose

    Ptwk-40s GCaMP6s T2A NLS::mNeptune unc-54 3' UTR C.elegans AVA,AVB and other neurons expression of GCaMP6 and nuclear mNeptune

    Depositor

    Mei Zhen

    Article

    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

    Insert

    GCaMP6
    

    Use

    Tags

    mNeptune

    Expression

    Worm

    Mutation

    Promoter

    Ptwk-40

    Available since

    May 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pZR047_Lenti-U6-sgGARP-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

    Plasmid

    #180268

    Purpose

    Lentiviral expression vector for CRISPRa-SAM with GARP targeting sgRNA

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    GARP sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTol2pA2-mnx1:MLS-KillerRed

    Plasmid

    #115515

    Purpose

    Plasmid driving expression of membrane bound KillerRed fluorophore in zebrafish motor neurons

    Depositor

    Marco Morsch

    Article

    Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

    Insert

    MLS-KillerRed
    

    Use

    Gateway cloning

    Tags

    MLS

    Expression

    Mutation

    Promoter

    -3mnx1

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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