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pHH0103_PLCG1-1/1

Plasmid

#91540

Purpose

Protein expression and purification of human SH3 domain construct PLCG1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PLCG1-1/1 (PLCG1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pBEL2809

Plasmid

#232510

Purpose

Expression plasmid of Cys-light LetA(L94C,I383C) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

YebST (letA, letB E. coli)

Tags

6xHis2xQH-TEV

Expression

Bacterial

Mutation

[6xHis2xQH-TEV-LetA1(1-223,L94C,C124S)]-[LetA2(22…

Promoter

araBAD

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL2820

Plasmid

#232511

Purpose

Expression plasmid of Cys-light LetA(Q180C,R380C) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

YebST (letA, letB E. coli)

Tags

6xHis2xQH-TEV

Expression

Bacterial

Mutation

[6xHis2xQH-TEV-LetA1(1-223,Q180C,C124S)]-[LetA2(2…

Promoter

araBAD

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ALX4-Flag

Plasmid

#215029

Purpose

Mammalian expression of human ALX4

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

ALX homeobox 4 (ALX4 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG_ALX4-Flag-HA

Plasmid

#215028

Purpose

Mammalian expression of human ALX4

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

ALX homeobox 4 (ALX4 Human)

Tags

Flag-HA

Expression

Mammalian

Promoter

CAG

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pQCXIP-SHMY-A1Pi

Plasmid

#182648

Purpose

Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), one tyrosine sulfation (TS) motif (Y), myc (M), His6 (H) and A1Pi sequence

Depositor

Martin Spiess

Article

Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

Insert

A1Pi (SERPINA1 Human)

Use

Retroviral

Tags

His6-tag, N-terminal ER targeting sequence of hem…

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 S111F-msfGFP

Plasmid

#180340

Purpose

mammalian expression of human SEPT9_i1 S111F fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 S111F

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 R106W-msfGFP

Plasmid

#180339

Purpose

mammalian expression of human SEPT9_i1 R106W fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 R106W

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 D10-25-msfGFP

Plasmid

#180334

Purpose

mammalian expression of human SEPT9_i1 D10-25 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 D10-25

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 DN7-msfGFP

Plasmid

#180335

Purpose

mammalian expression of human SEPT9_i1 DN7 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 DN7

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 NCmut2-msfGFP

Plasmid

#180330

Purpose

mammalian expression of human SEPT9_i1 NCmut2 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 I281D, M288D

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-f1(+)-SEC61B-dTomato

Plasmid

#115913

Purpose

donor to insert dTomato at the SEC61B locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

SEC61B-dTomato (SEC61B Human)

Use

Donor template

Tags

dTomato

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-f1(+)-TOMM20-dTomato

Plasmid

#115914

Purpose

donor to insert dTomato at the TOMM20 locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

TOMM20-dTomato (TOMM20 Human)

Use

Donor template

Tags

dTomato

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_CBX1_Chromo-shadow

Plasmid

#109965

Purpose

Protein expression and purification of CBX1_Chromo-shadow

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

CBX1_Chromo-shadow (CBX1 Human)

Expression

Bacterial

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_KARS_tRNA-anti-codon

Plasmid

#109865

Purpose

Protein expression and purification of KARS_tRNA-anti-codon

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

KARS_tRNA-anti-codon (KARS1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_LRP5_Ldl-recept-b

Plasmid

#109867

Purpose

Protein expression and purification of LRP5_Ldl-recept-b

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

LRP5_Ldl-recept-b (LRP5 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_EIF2S3_GTP-EFTU

Plasmid

#109829

Purpose

Protein expression and purification of EIF2S3_GTP-EFTU

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

EIF2S3_GTP-EFTU (EIF2S3 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_LRP5_Ldl-recept-b

Plasmid

#109785

Purpose

Protein expression and purification of LRP5_Ldl-recept-b

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

LRP5_Ldl-recept-b (LRP5 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-Mm_Plk5(300-595) PBD domain

Plasmid

#136316

Purpose

Mammalian expression of mouse Plk5-D300 (PBD domain 300-595) with N-terminal EGFP

Depositor

Guillermo de Cárcer, Marcos Malumbres

Article

de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

Insert

PLK5 (Plk5 Mouse)

Expression

Mammalian

Mutation

fragment 300-595

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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DLG3_PDZ_2

Plasmid

#103947

Purpose

Protein expression and purification of NE-DLG PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

DLG3 (DLG3 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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