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We narrowed to 5,457 results for: golden gate

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Showing: 2121 - 2140 of 5457 results

  5. pSEM399 - mosTI unc-119 - NNN - operon - mScarlet - nls - tbb-2

    Plasmid
    #182356
    Purpose
    Nuclear mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)
    Depositor
    Christian Froekjaer-Jensen
    Publication
    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068
    Type
    Empty backbone
    Expression
    Worm
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    182356-plasmid-map-sequence-id-361657

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  12. PX459v3

    Plasmid
    #178799
    Purpose
    SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Publication
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178799-plasmid-map-sequence-id-354842

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  13. pPD1178-LPDV promoterless PuroR-P2A-miRFP720

    Plasmid
    #201537
    Purpose
    Landing pad destination vector for mMoClo golden gate-based assembly of landing pad integration vectors; contains BxB1 attB site, promoterless puromycin resistance gene and miRFP720 gene
    Depositor
    Joshua Leonard
    Publication
    Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:
    Insert
    Promoterless PuroR-P2A-miRFP720
    Expression
    Mammalian
    Promoter
    None
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    201537-plasmid-map-sequence-id-401330

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  15. pJo55

    Plasmid
    #159623
    Purpose
    An empty HIS3 plasmid for translation factor eIF4E complementation test in yeast, strain Jo55. Golden Gate cloning using BsaI. Selection with RFP after cloning.
    Depositor
    Vasiliy Taranov
    Publication
    Lebedeva et al Biochemistry (Mosc). 2021 Sep;86(9):1128
    Type
    Empty backbone
    Expression
    Bacterial and Yeast
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    159623-plasmid-map-sequence-id-329054

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  19. pSB61 - pL0_V2 (CDS1)

    Plasmid
    #123183
    Purpose
    Golden Gate (MoClo; CDS1) compatible Tomato yellow leaf curl virus (TYLCV) V2 silencing suppressor based on NCBI Reference Sequence NC_004005
    Depositor
    Erin Cram, Carolyn Lee-Parsons
    Publication
    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi
    Insert
    Tomato yellow leaf curl virus (TYLCV) V2 (v2 Synthetic)
    Use
    Part for plant expression
    Expression
    Plant
    Mutation
    No BpiI and BsaI sites
    Available since
    May 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123183-plasmid-map-sequence-id-241416

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Showing: 2121 - 2140 of 5457 results