We narrowed to 3,663 results for: golden gate

1. L0-I14 DST GG4-GG5

   Plasmid

   #195896

   Purpose

   Level 0 DST (destabilization RNA tag: SAUR tag) with GG4 GG5 golden gate linkers

   Depositor

   Jennifer Nemhauser

   Article

   Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

   Insert

   DST
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMOD_D4800EC

   Plasmid

   #176876

   Purpose

   Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

   Depositor

   Daniel Voytas

   Article

   Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMOD_A4800EC

   Plasmid

   #176873

   Purpose

   Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

   Depositor

   Daniel Voytas

   Article

   Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMOD_B4800EC

   Plasmid

   #176874

   Purpose

   Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

   Depositor

   Daniel Voytas

   Article

   Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pZA52

   Plasmid

   #174765

   Purpose

   Entry clone for Golden Gate assembly

   Depositor

   Sophien Kamoun

   Article

   Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

   Insert

   GG_NbZAR1syn-L17E/D481V with STOP codon
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   L17E/D481V + STOP codon

   Promoter

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pZA50

   Plasmid

   #174763

   Purpose

   Entry clone for Golden Gate assembly

   Depositor

   Sophien Kamoun

   Article

   Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

   Insert

   GG_NbZAR1syn-D481V with STOP codon
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   D481V + STOP codon

   Promoter

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pZA51

   Plasmid

   #174764

   Purpose

   Entry clone for Golden Gate assembly

   Depositor

   Sophien Kamoun

   Article

   Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

   Insert

   GG_NbZAR1syn-L17E with STOP codon
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   L17E + STOP codon

   Promoter

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pZA49

   Plasmid

   #174762

   Purpose

   Entry clone for Golden Gate assembly

   Depositor

   Sophien Kamoun

   Article

   Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

   Insert

   GG_synthesized NbZAR1 (NbZAR1syn) with STOP codon
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   WT + STOP codon

   Promoter

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPD354 VP64-FRB (NLS)

   Plasmid

   #138846

   Purpose

   Constitutive expression of VP64-FRB under the CMV promoter.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   VP64-FRB
   

   Use

   Synthetic Biology

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPD353 VP16-FRB (NLS)

    Plasmid

    #138845

    Purpose

    Constitutive expression of VP16-FRB under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VP16-FRB
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPD098 ZF43i

    Plasmid

    #138780

    Purpose

    Constitutive expression of ZF43i under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43i
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEPQD0KN0244

    Plasmid

    #162282

    Purpose

    Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT, adds a N-terminal expression tag. Generates plasmids for T7-driven E. coli cell-free protein synthesis

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEPQD0KN0024

    Plasmid

    #162281

    Purpose

    Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG, adds a N-terminal expression tag. Generates plasmids for T7-driven E. coli cell-free protein synthesis

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLY133

    Plasmid

    #130961

    Purpose

    Engineered sgRNA-LEB4 generator including Anderson promoter J23100 for golden gate assembly

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Insert

    sgRNA-LEB4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Anderson promoter J23100

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLY131

    Plasmid

    #130956

    Purpose

    Engineered sgRNA-LEB2 generator including Anderson promoter J23100 for golden gate assembly

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Insert

    sgRNA-LEB2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Anderson promoter J23100

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLY132

    Plasmid

    #130960

    Purpose

    Engineered sgRNA-LEB3 generator including Anderson promoter J23100 for golden gate assembly

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Insert

    sgRNA-LEB3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Anderson promoter J23100

    Available since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLY130

    Plasmid

    #130952

    Purpose

    Engineered sgRNA-LEB1 generator including Anderson promoter J23100 for golden gate assembly

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Insert

    sgRNA-LEB1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Anderson promoter J23100

    Available since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEPOR1CB0104

    Plasmid

    #117569

    Purpose

    Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting AtIPCS1 {AT3G54020} in A. thaliana

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    Promoter_AtU6-26 + sa_gRNA_AtIPCS1 {AT3G54020} A. thaliana
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU6-26 (pICSL90002)

    Available since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEPOR1CB0093

    Plasmid

    #117568

    Purpose

    Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting AtIPCS1 {AT3G54020} in A. thaliana

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    Promoter_AtU6-26 + sgRNA_AtIPCS1 {AT3G54020} A. thaliana
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU6-26 (pICSL90002)

    Available since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEPOR1CB0103

    Plasmid

    #117567

    Purpose

    Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting AtCas6 {AT5G04770} in A. thaliana

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    Promoter_AtU6-26 + sgRNA_AtCas6 {AT5G04770} A. thaliana
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU6-26 (pICSL90002)

    Available since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge