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pGL4_10xUAS-CMVmin-BC0408-luc2

Plasmid

#227120

Purpose

Barcoded assay, UAS sensor for split TEV assays; barcode BC0408

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

10x clustered UAS element linked to CMV minimal promoter driving barcode 0408 and a luciferase reporter gene

Expression

Mammalian

Available Since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4_10xUAS-CMVmin-BC0441-luc2

Plasmid

#227129

Purpose

Barcoded assay, UAS sensor for split TEV assays; barcode BC0441

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

10x clustered UAS element linked to CMV minimal promoter driving barcode 0441 and a luciferase reporter gene

Expression

Mammalian

Available Since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4_CRE-CMVmin-BC0498-luc2

Plasmid

#227134

Purpose

Barcoded assay, cAMP/Ca2+ sensor; barcode BC0498

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

6x clustered CRE element linked to CMV minimal promoter driving barcode 0498 and a luciferase reporter gene

Expression

Mammalian

Available Since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4_10xUAS-CMVmin-BC0440-luc2

Plasmid

#227128

Purpose

Barcoded assay, UAS sensor for split TEV assays; barcode BC0440

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

10x clustered UAS element linked to CMV minimal promoter driving barcode 0440 and a luciferase reporter gene

Expression

Mammalian

Available Since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4_10xUAS-CMVmin-BC0442-luc2

Plasmid

#227130

Purpose

Barcoded assay, UAS sensor for split TEV assays; barcode BC0442

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

10x clustered UAS element linked to CMV minimal promoter driving barcode 0442 and a luciferase reporter gene

Expression

Mammalian

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pET-28b Salmonella NAT_6H C-terminal

Plasmid

#223436

Purpose

Salmonella NAT expression vector with C-terminal His6 tag

Depositor

Emily Balskus

Article

Mehta et al Nat Med. 2023 Mar;29(3):700-709. doi: 10

Insert

Salmonella NAT

Tags

His6

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-TRIF(pLxIS) S209A

Plasmid

#221245

Purpose

Expression of mutant TRIF pLxIS domain (1-222) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF pLxIS domain (1-222) (Ticam1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Mutation

S209A

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-TRIF(pLxIS) L193A

Plasmid

#221246

Purpose

Expression of mutant TRIF pLxIS domain (1-222) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF pLxIS domain (1-222) (Ticam1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Mutation

L193A

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pTol2_elavl3(HuC)_NES-Caprola_on-mEGFP

Plasmid

#194697

Purpose

HuC driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression in zebrafish

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_on-mEGFP

Use

Zebrafish expression

Tags

mEGFP

Promoter

elavl3(HuC)

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTol2_elavl3(HuC)_NES-Caprola_off-mEGFP

Plasmid

#194698

Purpose

HuC driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression in zebrafish

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_off-mEGFP

Use

Zebrafish expression

Tags

mEGFP

Promoter

elavl3(HuC)

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-DEWS

Plasmid

#208799

Purpose

Xenopus laevis His6-Myc-DONSON (D374A, E377A, W381A, S437A)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Tags

His and Myc

Expression

Bacterial

Mutation

D374A; E377A; W381A; S437A

Promoter

T7

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-3A

Plasmid

#208800

Purpose

Xenopus laevis His6-Myc-DONSON (V473A, R476A, Y481A)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Tags

His and Myc

Expression

Bacterial

Mutation

V473A; R476A; Y481A

Promoter

T7

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-Δ24

Plasmid

#208801

Purpose

Xenopus laevis His6-Myc-DONSON (1-24aa removed)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Tags

His and Myc

Expression

Bacterial

Mutation

Δ24, deletion of 1-24aa

Promoter

T7

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-DEW

Plasmid

#208802

Purpose

Xenopus laevis His6-Myc-DONSON (D374A, E377A, W381A)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Tags

His and Myc

Expression

Bacterial

Mutation

D374A; E377A; W381A

Promoter

T7

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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L_NLS-mutNLS V5-Twist

Plasmid

#201381

Purpose

Expresses Twist mutant for nucleocytoplasmic transport studies

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

Twist (Twist1 Mouse)

Tags

(NLS)SV40A4-V5

Expression

Mammalian

Mutation

K38R K73R

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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M_NLS-mutNLS V5-Twist

Plasmid

#201379

Purpose

Expresses Twist mutant for nucleocytoplasmic transport studies

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

Twist (Twist1 Mouse)

Tags

(NLS)SV40A5-V5

Expression

Mammalian

Mutation

K38R K73R

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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mutNLS V5-Twist

Plasmid

#201380

Purpose

Expresses Twist mutant for nucleocytoplasmic transport studies

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

Twist (Twist1 Mouse)

Tags

V5

Expression

Mammalian

Mutation

K38R K73R

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
H_NLS-mutNLS V5-Twist

Plasmid

#201378

Purpose

Expresses Twist mutant for nucleocytoplasmic transport studies

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

Twist (Twist1 Mouse)

Tags

(NLS)SV40-V5

Expression

Mammalian

Mutation

K38R K73R

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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UL_NLS-mutNLS V5-Twist

Plasmid

#201382

Purpose

Expresses Twist mutant for nucleocytoplasmic transport studies

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

Twist (Twist1 Mouse)

Tags

(NLS)SV40A3-V5

Expression

Mammalian

Mutation

K38R K73R

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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mut GBP2 V5-Twist

Plasmid

#201377

Purpose

Expresses Twist mutant for nucleocytoplasmic transport studies

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

Twist (Twist1 Mouse)

Tags

V5

Expression

Mammalian

Mutation

Y107E

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge