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Plasmid#225586PurposeMammalian expression vector for Doxycycline inducible expression of F8.2(S54L+T127A) anti-tau nanobody with a self-cleaving T2A-mCherry fluorescent expression reporterDepositorInsertF8.2(S54L+T127A)-T2A-mCherry
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 CMV 3xFG eIF4G1
Plasmid#158775PurposepcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform lacking the microexon.DepositorInserteIF4G1
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 rtTA BirA eIF4G
Plasmid#158789PurposepcDNA5 based transfection plasmid for the exogenous expression of N-terminal BirA-Flag-tagged eIF4G1 lacking the microexon for generation of N2A FlpIn cell lines for BioIDDepositorInserteIF4G1
UseTags3xFlag and BirA*ExpressionMammalianMutationPromoterminiCMVAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 GIL-11 myc TEV TwinStrep
Plasmid#199627PurposeEucaryotic expression plasmid for chimeric cytokine of IL-11 and LIF (GIL-11) with a TwinStrep-tagDepositorInsertGIL-11
UseTagsTwinStrep-tagExpressionMammalianMutationPromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphaoA-RLuc8
Plasmid#140976PurposeEncodes a G alpha subunit (GNAO1 Isoform Alpha 1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphaoA-RLuc8 (GNAO1 Human)
UseLuciferaseTagsExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphasL-RLuc8
Plasmid#140981PurposeEncodes a G alpha subunit (GNAS1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphasL-RLuc8 (GNAS Human)
UseLuciferaseTagsExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlpha12-RLuc8
Plasmid#140985PurposeEncodes a G alpha subunit (GNA12) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlpha12-RLuc8 (GNA12 Human)
UseLuciferaseTagsExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphaoB-RLuc8
Plasmid#140977PurposeEncodes a G alpha subunit (GNAO1 Isoform Alpha 2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphaoB-Rluc8 (GNAO1 Human)
UseLuciferaseTagsExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/(CAA)26 5′ leader GUG-nLuc-3XFLAG
Plasmid#127325PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with (CAA)26 repeats in 5' UTRDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/(CAA)26 5′ leader AUG-nLuc-3XFLAG
Plasmid#127324PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with (CAA)26 repeats in 5' UTRDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only