We narrowed to 5,380 results for: kras

1. pDF0317 HvsCas7-11 mammalian expression

   Plasmid

   #172511

   Purpose

   Encodes human codon optimized HvsCas7-11 protein alone for expression in mammalian cells

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   HvsCas7-11 mammalian expression
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5FRT/TO-HOIP-Strep-HA

   Plasmid

   #113494

   Purpose

   Expression of human HOIP with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   HOIP (RNF31 Human)
   

   Use

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAS

   Plasmid

   #206994

   Purpose

   Plasmid expressing both SpCas9 and SaCas9

   Depositor

   Alejandro Chavez

   Article

   Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

   Insert

   SpCas9 - P2A - SaCas9 (NEWENTRY, NEWENTRY )
   

   Use

   Tags

   P2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRDA_889

   Plasmid

   #216080

   Purpose

   ITRs from AAV2, destination vector

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   ITRs from AAV2
   

   Use

   AAV; Destination

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CK(0.4)-CRY2PHR-P2A-CIBN-mCherry-VAMP2

   Plasmid

   #178576

   Purpose

   AAV vector for CK(0.4)::Opto-vTrap

   Depositor

   Won Do Heo

   Article

   Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

   Insert

   CRY2PHR-P2A-CIBN-mCherry-VAMP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CamKIIa

   Available since

   April 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLJM1-FLAG-metap2

   Plasmid

   #100510

   Purpose

   Expresses FLAG tagged metap2 in mammalian cells to make lentivirus

   Depositor

   David Sabatini

   Article

   Gu et al Science. 2017 Nov 10;358(6364):813-818.

   Insert

   METAP2 (METAP2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMV_AA050

   Plasmid

   #216107

   Purpose

   gDNA expression for Cas9 with ChenF+E trRNA (guide only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   gDNA expression for Cas9 with ChenF+E trRNA
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBAD_His B_QuasAr2

   Plasmid

   #64134

   Purpose

   Fluorescent reporter of membrane voltage with improved sensitivity

   Depositor

   Adam Cohen

   Article

   Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

   Insert

   QuasAr2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRDA_936

   Plasmid

   #216094

   Purpose

   Cas12a [EnAs] knockout targeting CD46, CD47, CD55, CD81, positive control

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   CD46, CD47, CD55, CD81 guides
   

   Use

   CRISPR and Lentiviral; Positive controls

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB-cT3G-cERH-ZNF281

    Plasmid

    #222923

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of ZNF281

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    ZNF281 (ZNF281 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-CMV-cGAS-N-HA

    Plasmid

    #130913

    Purpose

    Expresses human cGAS N terminus(aa1-159)-HA; Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    cGAS N terminus
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. LLP786_SpyCatcher-EZH2-FL-CO

    Plasmid

    #211779

    Purpose

    SpyTag counterpart binding domain, SpyCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    aGCN4-KRAB
    

    Use

    Tags

    3xTy1

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect KRAB e…

    Promoter

    pEF1a and pSV40

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIND(V5)EcoDam

    Plasmid

    #59201

    Purpose

    Mammalian DamID vector used to fuse gene of interest to V5-tagged E.coli Dam. Can also express Dam alone as negative control.

    Depositor

    Bas van Steensel

    Article

    Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

    Type

    Empty backbone

    Use

    Damid

    Tags

    Dam (DNA adenine methyltransferase) and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Heat Shock Minimal Promoter

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSCV-BirA-P2A-mCherry

    Plasmid

    #74056

    Purpose

    retroviral expression plasmid for selfcleaving E.coli biotin ligase BirA-P2A-mCherry fusion protein

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    BirA biotin ligase
    

    Use

    Retroviral

    Tags

    P2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV-LTRs

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pProEx Rab5C(S30C) CL

    Plasmid

    #224273

    Purpose

    Protein purification of Rab5C (S30C)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    Rab5C (RAB5C Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S30C

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLJC5-Flag-SAMTOR

    Plasmid

    #102420

    Purpose

    3rd generation lentiviral vector expressing FLAG SAMTOR in mammalian cells driven by UBC promoter

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    SAMTOR (BMT2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQCXIX_sh_HOIP/RNF31_#6

    Plasmid

    #75252

    Purpose

    retroviral shRNA vector against human HOIP/RNF31, expresses GFP for transduction control

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    shRNA against human RNF31 (RNF31 )
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    June 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. AA031

    Plasmid

    #216011

    Purpose

    Fragmid fragment: (Cas protein) nickase Cas for base editing

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    nCas9-SpRY (D10A)_v1.1 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. AA030

    Plasmid

    #216010

    Purpose

    Fragmid fragment: (Cas protein) nickase Cas for base editing

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    nCas9-SpG (D10A)_v1.1 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAT9651-BEAR-GFP

    Plasmid

    #162989

    Purpose

    BEAR target plasmid with split EGFP and disrupted 5' splice site

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    EGFP split with an intron between amino acids 95-96
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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