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pXD80EN15-DadhA(cHis8)BC-DadRS-noAccs(ElA2)

Plasmid

#225327

Purpose

Expresses Eggerthella lenta A2 Dadh without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh

Tags

His8

Expression

Bacterial

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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hVEGF165-eGFP-hVEGF165 glycosylation sequon-His tag

Plasmid

#240484

Purpose

Express engineered glycosylated eGFP in CHO cells

Depositor

Xi Ren

Article

Xing et al Acta Biomater. 2025 Nov;207:221-232. doi

Insert

glycosylated eGFP

Tags

3 repeats of hVEGF165 glycosylation sequon, 6xHis…

Expression

Mammalian

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-N'-EGFP-ATG9A-IRES-blast

Plasmid

#222518

Purpose

Expresses N'EGFP-ATG9A in mammalian cells

Depositor

Alicia Pickrell

Insert

ATG9A (ATG9A Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pSC2+-HyPerFLEX-Lifeact

Plasmid

#217568

Purpose

Mammalian expression HyPerFLEX targeted to actin filaments

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-Lifeact

Expression

Mammalian

Promoter

CMV IE94

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAVCam1.3_smFP_Myc

Plasmid

#201205

Purpose

AAV vector for camKII promoter (1.3kb)-driven smFP_Myc (Myc-tagged spaghetti monster) expression

Depositor

Tetsuo Yamamori

Article

Watakabe et al J Vis Exp. 2025 Jan 17;(215). doi: 10.37

Insert

smFP _Myc (from addgene #59757)

Use

AAV

Expression

Bacterial and Mammalian

Promoter

mouse CamKII (1.3kb)

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAAVCam1.3_smFP_HA

Plasmid

#201206

Purpose

AAV vector for camKII promoter (1.3kb)-driven smFP_HA (HA-tagged spaghetti monster) expression

Depositor

Tetsuo Yamamori

Article

Watakabe et al J Vis Exp. 2025 Jan 17;(215). doi: 10.37

Insert

smFP _HA (from addgene #59759)

Use

AAV

Expression

Bacterial and Mammalian

Promoter

mouse CamKII (1.3kb)

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAAVCam1.3_smFP_FLAG

Plasmid

#201207

Purpose

AAV vector for camKII promoter (1.3kb)-driven smFP_FLAG (FLAG-tagged spaghetti monster) expression

Depositor

Tetsuo Yamamori

Article

Watakabe et al J Vis Exp. 2025 Jan 17;(215). doi: 10.37

Insert

smFP _FLAG (from addgene #59756)

Use

AAV

Expression

Bacterial and Mammalian

Promoter

mouse CamKII (1.3kb)

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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AAVTRE3_smFP_FLAG

Plasmid

#201210

Purpose

AAV vector for TRE(TRE3G)-driven smFP_FLAG (FLAG-tagged spaghetti monster) expression

Depositor

Tetsuo Yamamori

Article

Watakabe et al J Vis Exp. 2025 Jan 17;(215). doi: 10.37

Insert

smFP _FLAG (from addgene #59756)

Use

AAV

Expression

Bacterial and Mammalian

Promoter

TRE3G

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF9-Pct3-(–)-Eldh2(cHis6)-Accs(ElA2)

Plasmid

#233868

Purpose

Expresses His-tagged Eggerthella lenta A2 (–)-Eldh2 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eldh2

Tags

His

Expression

Bacterial

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80EN16-HcdhA(cHis8)BC-partial cluster(ElA2)

Plasmid

#225328

Purpose

Expresses Eggerthella lenta A2 Hcdh partial gene cluster (inactive) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Hcdh partial gene cluster

Tags

His8

Expression

Bacterial

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80EN6-Elen0497-ElVadh-noAccs(ElDSM2243)

Plasmid

#225314

Purpose

Expresses Eggerthella lenta DSM 2243 El Vadh without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

El Vadh (Elen_0497 Eggerthella lenta)

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD70EN20-Pct5-GPA_25810-(+)-Eldh1(GpDSM19378)

Plasmid

#225306

Purpose

Expresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 in Eggerthella lenta DSM 2243 with a cumate inducible promoter

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eldh1

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD70EN21-Pct5-GPA_25800-(+)-Eldh2(GpDSM19378)

Plasmid

#225307

Purpose

Expresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh2 in Eggerthella lenta DSM 2243 with a cumate inducible promoter

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eldh2

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF25-Pct3-(–)-Eddh(nHis6)(G3C)

Plasmid

#233873

Purpose

Expresses His-tagged Gordonibacter pamelaeae 3C (–)-Eddh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eddh

Tags

His

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF30-DadhABC(cHis8)RS-Accs(Cadh)(ElA2)

Plasmid

#233876

Purpose

Expresses Eggerthella lenta A2 Dadh with the accessory genes from the cadh gene cluster in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh

Tags

His8

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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L1p1_min35S_Luciferase

Plasmid

#231035

Purpose

Cloning vector to clone promoter fragment in front of minimal p35S:Luciferase:tNOS

Depositor

Wilma van Esse

Article

Winkelmolen et al Plant Physiol. 2025 Aug 4;198(4):kiaf309

Insert

Minimal p35S:Luciferase:tNOS

Use

Luciferase

Expression

Bacterial

Promoter

minimal p35S

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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pGGA-pTRE-B

Plasmid

#218894

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

TRE

Use

Synthetic Biology

Mutation

580G>C

Available Since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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pGGF-bGH polyA-G

Plasmid

#218902

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

bGH polyA

Use

Synthetic Biology

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGGF-WPRE-G

Plasmid

#218901

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

WPRE

Use

Synthetic Biology

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGGE-V5_TurboID-F

Plasmid

#218900

Purpose

golden gate cloning

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

V5-TurboID

Use

Synthetic Biology

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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