We narrowed to 5,822 results for: pCas

1. pCAG-mIRFP670nuc-TEV-Xph20-ETWV

   Plasmid

   #133044

   Purpose

   Expresses Xph20-ETWV in mammalian cells with nuclear miRFP670 as reporter

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

   Insert

   Xph20-ETWV
   

   Tags

   NLS, TEV protease + TEV cleavage site, and miRFP6…

   Expression

   Mammalian

   Mutation

   S63K

   Promoter

   CAG

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS SARS-CoV-2 Alpha Spike

   Plasmid

   #185691

   Purpose

   Expresses codon-optimized full length SARS-CoV-2 Alpha Spike

   Depositor

   Marceline Côté

   Article

   Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi

   Insert

   SARS-CoV-2 Alpha Spike (S )
   

   Expression

   Mammalian

   Mutation

   Contains the following mutations: Δ69-70, Δ145, N…

   Promoter

   chicken β-actin promoter, CMV enhancer

   Available Since

   June 8, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pU6-SCGB3A2_gRNA1-SpCas9-T2A-GFP

   Plasmid

   #126698

   Purpose

   to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human SCGB3A2 locus at the end of the endogenous coding sequence

   Depositor

   Darrell Kotton

   Article

   McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

   Insert

   sgRNA targeting human SCGB3A2 locus
   

   Expression

   Mammalian

   Promoter

   Human U6

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmax-3xFLAG_3xNLS_dPspCas13b(AAAA)_2xNLS-Clover

   Plasmid

   #196829

   Purpose

   Express dpdpCas13b with AAAA mutation and Clover

   Depositor

   Albert Cheng

   Article

   Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

   Insert

   dpspCas13b(AAAA)
   

   Tags

   3XFLAG and NES

   Expression

   Mammalian

   Mutation

   K367A, D369A and K370A

   Available Since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpCas9-NRCH-APOBEC-nCas9-Ung

   Plasmid

   #198552

   Purpose

   Expresses human codon-optimized SpCas9-NRCH-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NRCH-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRCH-APOBEC-nCas9-Ung
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-TWIN-STREP-FLAG-FLCN

   Plasmid

   #164981

   Purpose

   FLCN expressing vector for mammalian cells

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

   Insert

   FLCN (FLCN Human)
   

   Tags

   2xSTREP FLAG

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-mCherry-FKBP-GAP(ARHGAP29)

   Plasmid

   #222632

   Purpose

   Rho inactivation using rapamycin

   Depositor

   Arnold Hayer

   Article

   Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

   Insert

   mCherry-FKBP-GAP(ARHGAP29) (ARHGAP29 Human)
   

   Tags

   mCherry-FKBP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG_NLS-Cas9-XTEN-Ec73RT-NLS_pA_pCMV_BFP_pA

   Plasmid

   #176458

   Purpose

   Vector for encoding a human codon-optimized SpCas9-XTEN-Ec73RT fusion driven by CAG promoter and tagBFP driven by CMV promoter

   Depositor

   Hui Yang

   Article

   Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

   Insert

   humanized S. pyogenes Cas9-XTEN-Ec73RT fusion
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pspCas9-3'UTR-Tetra-com-vector

   Plasmid

   #132553

   Purpose

   Expresses spCas9 and sgRNA scaffold with com aptamer replacing the Tetraloop

   Depositor

   Baisong Lu

   Article

   Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

   Inserts

   Cas9
   sgRNA scaffold with com replacing the Tetraloop
   

   Expression

   Mammalian

   Available Since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-FLAG-IRES-blasticidin-Sox2 WT

    Plasmid

    #182046

    Purpose

    expression of Sox2

    Depositor

    Hyonchol Jang

    Article

    Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

    Insert

    Sox2 (Sox2 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-OSF-PP-human-CHMP3(150)

    Plasmid

    #154181

    Purpose

    expresses human CHMP3(150) in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Insert

    CHMP3 (CHMP3 Human)
    

    Tags

    FLAG and Strep-Tag II

    Expression

    Mammalian

    Mutation

    C-terminal truncation of CHMP3 at amino acid 150

    Promoter

    CAG

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-miRFP670-pA_U6-BbsI (pE059)

    Plasmid

    #149353

    Purpose

    Mammalian expression vector for miRFP670

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    miRFP670
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS-Twin-Strep-TEV-DGKζ

    Plasmid

    #202531

    Purpose

    Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human diacylglycerol kinase ζ (DGKζ) in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Insert

    DGKZ (DGKZ Human)
    

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Promoter

    CAG and chicken β-actin promoter

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-H2B-mRuby3-IRES-eGtACR1-ST

    Plasmid

    #108960

    Purpose

    Anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequence

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    eGtACR1-ST
    

    Expression

    Mammalian

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAGGS-SMS1-TEV-Twin-Strep

    Plasmid

    #202528

    Purpose

    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Insert

    SGMS1 (SGMS1 Human)
    

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Mutation

    deleted stop codon (TAA)

    Promoter

    CAG and chicken β-actin promoter

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHBS952 TARDBP-Exon1-ko-sgRNA1-SpCas9

    Plasmid

    #107857

    Purpose

    To knock-out TDP43

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    gRNA against TARDBP (Exon 1) (TARDBP Human)
    

    Expression

    Mammalian

    Available Since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCA15-Ubc-NLS- scFV-tdTomato

    Plasmid

    #199446

    Purpose

    Expression of scFV-tdTomato that binds to the GCN4 peptide from the SunTag System in mammalian cells

    Depositor

    Baohui Chen

    Article

    Xu et al J Virol. 2022 Dec 21;96(24):e0092022. do

    Insert

    scFV-tdTomato
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    human ubiquitin C (UBC)

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS-mNG-eDHFR(69K6)-G-alpha-q

    Plasmid

    #172106

    Purpose

    Mammalian expression of Gαq fused to mNeonGreen-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    mNeonGreen-eDHFR(69K6)-Gαq (GNAQ Human)
    

    Expression

    Mammalian

    Mutation

    Gαq: C9S/C10S/Q209L

    Promoter

    CAG

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-mCherry-FKBP-GEF(ITSN1)

    Plasmid

    #222631

    Purpose

    Cdc42 activation using rapamycin

    Depositor

    Arnold Hayer

    Article

    Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

    Insert

    mCherry-FKBP-GEF(ITSN1) (ITSN1 Human)
    

    Tags

    mCherry-FKBP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-ULK1 (836-1050)-MBP

    Plasmid

    #203562

    Purpose

    Mammalian expression of ULK1 (836-1050) with MBP tag

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    ULK1 (ULK1 Human)
    

    Tags

    MBP-10His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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