We narrowed to 5,654 results for: pCas

1. pX330-Flag-wtSpCas9 (without sgRNA)

   Plasmid

   #92353

   Purpose

   Expression plasmid for human codon-optimized wild-type SpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   3xFlag-NLS-wild-type SpCas9-NLS
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBT250_(pCA-G-intron(Neo)-tTA2)

   Plasmid

   #36876

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   GFP
   tTA2
   beta-globin intron
   Neo
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deleted nucleotides after nucleotide 274, inserti…

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer…

   Available since

   Aug. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-sfGFP-GSAx9-iCre-ERT2

   Plasmid

   #125748

   Purpose

   sfGFP and iCreERT2 fused by GSAx9 linker

   Depositor

   Mario Capecchi

   Article

   Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

   Insert

   sfGFP-GSAx9-iCre-ERT2
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBT241_(pCA-ATG-intron-tTA2)

   Plasmid

   #36875

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Insert

   tTA2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   insertion of a beta-globin intron with a loxP sit…

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer)

   Available since

   June 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAA)

   Plasmid

   #137859

   Purpose

   Monomeric TagBFP2 and iCre-ERT2 recombinase (PAA N-terminus)

   Depositor

   Mario Capecchi

   Insert

   mTagBFP2-T2A-iCreERT2
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-LplA(AILR,36)-NRX3b D137A

   Plasmid

   #43920

   Depositor

   Alice Ting

   Article

   Liu et al PLoS One. 2013;8(2):e52823. doi: 10.1371

   Insert

   AILR-LplA36-NRX3b (lplA Synthetic, Human)
   

   Use

   Tags

   2XHA

   Expression

   Mammalian

   Mutation

   D137A

   Promoter

   Chicken Beta Actin

   Available since

   June 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-EGFP/RFP-miR-E2-4int

   Plasmid

   #19820

   Depositor

   Zuoshang Xu

   Article

   Qiu et al BMC Biotechnol. 2008 Oct 7;8:77. doi: 10

   Insert

   miRNA
   

   Use

   Cre/Lox and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pCAGGS-CIBN-p53CT(98-393)-mNeonGreen

   Plasmid

   #241844

   Purpose

   Expresses a localizer of Opto-p53 in mammalian cells.

   Depositor

   Kazuhiro Aoki

   Article

   Tsuruoka et al Cell Struct Funct. 2025 Jun 12. doi: 10.

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   CIBN and mNeonGreen

   Expression

   Mammalian

   Mutation

   C-terminus (97-393 aa)

   Promoter

   CAGGS promoter

   Available since

   Sept. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSPcas9(BB)-2A-Puro V2.0 sgCHMP2B-2 aauucccaaaugaagauggc

   Plasmid

   #231998

   Purpose

   Expression of an sgRNA targeting CHMP2B, Cas9, and a PuroR marker

   Depositor

   Franz-Ulrich Hartl

   Insert

   CHMP2B-targeted sgRNA (CHMP2B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-pCAG-iCRE-2A-H2BGFP-WPRE

    Plasmid

    #234893

    Purpose

    CAG-driven Cre recombinase coupled with H2B-GFP for nuclear fluorescent labelling

    Depositor

    Silvia Arber

    Article

    Falasconi et al Nature. 2025 May 28. doi: 10.1038/s41586

    Insert

    iCRE-2A-H2BGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCBh-T7-SpCas9-P2A-mTagBFP2(RAS3575)

    Plasmid

    #223111

    Purpose

    pCBh Human expression plasmid for SpCas9 with with C-term BPNLS-P2A-mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9-P2A-mTagBFP2
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS-MTS(S17/43A)-TFAM-V5

    Plasmid

    #202404

    Purpose

    expresses MTS(S17/43A)-TFAM-V5 in mammalian cells

    Depositor

    Hung-Chun Chang

    Insert

    Transcription Factor A, Mitochondrial (TFAM Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    S17A, S43A

    Promoter

    CAG

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS-MTS(S17/43A)-EGFP-V5

    Plasmid

    #200935

    Purpose

    expresses MTS(S17/43A)-EGFP-V5 in mammalian cells

    Depositor

    Hung-Chun Chang

    Insert

    EGFP
    

    Use

    Tags

    V5 and mitochondrial targeting sequence (MTS)

    Expression

    Mammalian

    Mutation

    S17A, S43A in MTS

    Promoter

    CAG

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-GPHN-FingR-mGold-CCR5TC

    Plasmid

    #231784

    Purpose

    Visualization of inhibitory post-synapse using GPHN-FingR-mGold

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    GPHN-FingR-mGold-CCR5TC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Actin promoter with CMV enhancer

    Available since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-GPHN-FingR-mVenus-CCR5TC

    Plasmid

    #231783

    Purpose

    Visualization of inhibitory post-synapse using GPHN-FingR-mVenus

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    GPHN-FingR-mVenus-CCR5TC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Actin promoter with CMV enhancer

    Available since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-GPHN-FingR-mGold2t-CCR5TC

    Plasmid

    #231786

    Purpose

    Visualization of inhibitory post-synapse using GPHN-FingR-mGold2t

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    GPHN-FingR-mGold2t-CCR5TC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Actin promoter with CMV enhancer

    Available since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGEN-Dre-nls (JDW 1131)

    Plasmid

    #229809

    Purpose

    A CAGGS-driven dre recombinase followed by a c-terminal 1x nls sequence

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    DRE recombinase-nls
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS puro human RLIM N581K

    Plasmid

    #232242

    Purpose

    Mammalian expression of Human RLIM N581K

    Depositor

    Francisco Bustos

    Article

    Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

    Insert

    Human RLIM N581K (RLIM Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N581K

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS-Twin-strep-TEV-DGKδ2

    Plasmid

    #223720

    Purpose

    Express human diacylglycerol kinase delta 2 protein (N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell

    Depositor

    Chiaki Murakami

    Insert

    DGKD (DGKD Human)
    

    Use

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Mutation

    deleted start codon

    Promoter

    CAG and chicken β-actin promoter

    Available since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAGGS-PHOSPHO2-TEV-Twin-Strep

    Plasmid

    #222016

    Purpose

    Express human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell

    Depositor

    Chiaki Murakami

    Insert

    PHOSPHO2 (PHOSPHO2 Human)
    

    Use

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Mutation

    deleted stop codon (TAA)

    Promoter

    chicken β-actin promoter

    Available since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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