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pLV-EF1a-ArgSen (-)P2A (-)NLS

Plasmid

#184699

Purpose

Lentiviral transfer plasmid for expression of ArgSen (-)P2A (-)NLS

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

ArgSen (-)P2A (-)NLS

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4D41

Plasmid

#185841

Purpose

Testing the CYC1 core promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1-PCYC1+[Z268]>yEGFP>TPGK1-TURA3

Expression

Yeast

Mutation

None

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP10CF72 (B11)

Plasmid

#185859

Purpose

Testing the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1- PSkGAL2M3(2*PZ4)>(BamHI)yEGFP>TURA3

Expression

Yeast

Mutation

None

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP8EFA

Plasmid

#185854

Purpose

Testing a heat inducible protein degradation tag -DHFR P66L using a green fluorescent protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1-PTEF1>UBI4>RHGSGTMV>DHFRP66L>yEGFP>TPGK1-TURA3

Expression

Yeast

Mutation

DHFR P66L

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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KBB221

Plasmid

#185071

Purpose

SST::ACT2 "SA236"

Depositor

Ken Belanger

Article

Belanger et al Planta. 2003 Oct;217(6):931-50. doi: 10.

Insert

ACT2

Expression

Yeast

Mutation

ACT2-SA236

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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KBB215

Plasmid

#185068

Purpose

SST::WAK2 EcoRI/ScaI fragment (secretion confirmed)

Depositor

Ken Belanger

Article

Belanger et al Planta. 2003 Oct;217(6):931-50. doi: 10.

Insert

WAK2

Expression

Yeast

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pVH616

Plasmid

#169605

Purpose

Tier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (PmPGK1-Fluc-pA::A2-pA::PCREm4-Nluc-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette

Expression

Mammalian

Promoter

PCREm4 / PPGK

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pVH270-Tier1-OTtgO2-PCMVmin2-SEAP-p2A-iRFP670

Plasmid

#169592

Purpose

Tier-1 vector encoding PTtgO2-driven SEAP-p2A-iRFP670 expression (OTtgO2-PCMVmin-2-SEAP-p2A-iRFP670-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

phloretin-controlled SEAP and iRFP expression

Expression

Mammalian

Promoter

TtgO2-PCMVmin-2

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDIV492

Plasmid

#177700

Purpose

Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.

Depositor

Uffe Mortensen

Article

Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

Inserts

Cas9
Nat

Use

CRISPR

Tags

SV40

Expression

Bacterial and Yeast

Promoter

RHR2p (Debaryomyces hansenii ) and TEF1p (Ashbya …

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pVH266-Tier1-OTetO7-PCMVmin2-SEAP-sTRSV

Plasmid

#169589

Purpose

Tier-1 vector encoding PTetO7-driven SEAP expression with a 3'UTR sTRSV ribozyme (OTetO7-PCMVmin-2-SEAP-3'UTRsTRSV-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled SEAP expression

Expression

Mammalian

Promoter

TetO7-PCMVmin-2

Available Since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

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