We narrowed to 329 results for: pgp

1. pGP-20XUAS-IVS-Syn21-jGCaMP8m-p10

   Plasmid

   #162387

   Purpose

   Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Insert

   jGCaMP8m
   

   Use

   Tags

   6xHis

   Expression

   Insect

   Mutation

   A25G F286Y

   Promoter

   hsp70

   Available since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE

   Plasmid

   #104496

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Has Service

   AAV PHP.eB, AAV Retrograde, and AAV1

   Insert

   jGCaMP7f
   

   Use

   AAV and Cre/Lox

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGP-AAV-syn-FLEX-jGCaMP7b-WPRE

   Plasmid

   #104493

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switch

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Has Service

   AAV Retrograde and AAV1

   Insert

   jGCaMP7b
   

   Use

   AAV and Cre/Lox

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGP-AAV-CAG-FLEX-jGCaMP7b-WPRE

   Plasmid

   #104497

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Insert

   jGCaMP7b
   

   Use

   AAV and Cre/Lox

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRI008-PgpdA-Cas12aScaffold-BsmbI-Cas12aScaffold-TrcpT

   Plasmid

   #140201

   Purpose

   Fungal vector for one-step cloning of LbCas12a crRNA arrays and expression. Pgpda and pyroA are BsmbI domesticated.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology; Cloning of crrna an…

   Tags

   dLbCas12a crRNA scaffold

   Expression

   Mutation

   Promoter

   PgpdA

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRI011-pYFAC-pyroA-PgpdA-4xcrRNAarrayelcA-TtrpC

   Plasmid

   #140203

   Purpose

   Fungal vector to express an LbCas12a crRNA array targeted to Pelca, to be contransformed with pCRI009 containing PelcA-mCherry in a dLbCas12a-VPR strain as proof-of-concept of CRISPRa.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Insert

   crRNA array elcA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PgpdA

   Available since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRI007-pGEM-PgpdA-Cas12aScaffold-BsmbI-TtrpC

   Plasmid

   #140200

   Purpose

   Cloning vector for LbCas12a crRNAs ( Step 1 of the 2-step cloning alternative). Full PgpdA sequence is reconstituted when amplifying the expression cassette for cloning in a fungal vector.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   dLbCas12a crRNA scaffold

   Expression

   Mutation

   Promoter

   PgpdA

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGP-AAV-CAG-flex-iGABASnFR2n-WPRE

   Plasmid

   #218881

   Purpose

   AAV-mediated expression of improved GABA sensor (negative change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Insert

   iGABASnFR2n
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   S99A F104H R168P

   Promoter

   CAG

   Available since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGP-AAV-GFAP-iGABASnFR2(no bind)-WPRE

   Plasmid

   #218873

   Purpose

   AAV-mediated expression of improved GABA sensor control (no change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Has Service

   AAV1 and AAV5

   Insert

   iGABASnFR2(no bind)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   S99A F102G R168P

   Promoter

   GFAP

   Available since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGP-AAV-syn-iGABASnFR2(no bind)-WPRE

    Plasmid

    #218876

    Purpose

    AAV-mediated expression of improved GABA sensor control (no change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Has Service

    AAV1 and AAV5

    Insert

    iGABASnFR2(no bind)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    S99A F102G R168P

    Promoter

    Synapsin

    Available since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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