We narrowed to 508 results for: transposase

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pUCSh3-2300

Plasmid

#170985

Purpose

Encodes sgRNA target 2300 and Tetracycline resistance transposon.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

Sh-CAST sgRNA 2300

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pUCSh8-2300

Plasmid

#170986

Purpose

Encodes sgRNA target 2300 and Hygromycin resistance transposon.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

Sh-CAST sgRNA 2300

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pJY3919

Plasmid

#113332

Purpose

Hermes transposable plasmid; TIR-flanked LEU2, recoded transposase driven by TEF1 promoter

Depositor

Suzanne Sandmeyer

Article

Patterson et al Metab Eng. 2018 May 22;48:184-196. doi:

Inserts

beta-isopropyl malate dehydrogenase
codon-optimized Hermes transposase

Expression

Yeast

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pDonorGFP(Ptr)

Plasmid

#175578

Purpose

DNA donor for transposition

Depositor

Sheng Yang

Article

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

Mini-Tn, GFP cassette

Expression

Bacterial

Mutation

NO

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pU6_N7-sgRNA2

Plasmid

#190664

Purpose

Expresses U6-driven N7 sgRNA2 (poly-T mutated out), BsmbI dropout cassette for cloning

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

N7 sgRNA2

Expression

Mammalian

Promoter

U6

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pDonorCm(Ptr)

Plasmid

#175577

Purpose

DNA donor for transposition

Depositor

Sheng Yang

Article

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

Mini-Tn, Cm cassette

Expression

Bacterial

Mutation

NO

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pMT3928

Plasmid

#113331

Purpose

control for Hermes transposition; LEU2 without flanking Hermes TIRs, recoded transposase driven by TEF1 promoter

Depositor

Suzanne Sandmeyer

Article

Patterson et al Metab Eng. 2018 May 22;48:184-196. doi:

Inserts

beta-isopropyl malate dehydrogenase
codon-optimized Hermes transposase

Use

Unspecified

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pSIBR4-Cre

Plasmid

#219435

Purpose

Theophylline_induced self_splicing intron_based riboswitch for the moderate expression of Cre recombinase

Depositor

Sheng Yang

Article

Xu et al Biotechnol Bioeng. 2024 Mar;121(3):1163-

Insert

intron 4-Cre

Expression

Bacterial

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pUCSh3-mRFP

Plasmid

#170983

Purpose

Encodes sgRNA scaffold with mRFP in place of target and Tetracycline resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

mRFP

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pUCSh2-mRFP

Plasmid

#170982

Purpose

Encodes sgRNA scaffold with mRFP in place of target and Kanamycin resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

mRFP

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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