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pBEL2201

Plasmid

#170408

Purpose

Genetic complementation plasmid of LetA-[LetB PLL6->5] mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Expression

Bacterial

Mutation

LetA-LetB(Δ702-716, replace with 580-600)

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2254

Plasmid

#170409

Purpose

Genetic complementation plasmid of LetA-[LetB PLL6->2] mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Expression

Bacterial

Mutation

LetA-LetB(Δ700-717, replace with 225-249)

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2255

Plasmid

#170410

Purpose

Genetic complementation plasmid of LetA-[LetB PLL6->4] mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Expression

Bacterial

Mutation

LetA-LetB(Δ700-716, replace with 445-479)

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2256

Plasmid

#170411

Purpose

Genetic complementation plasmid of LetA-[LetB PLL6->7] mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Expression

Bacterial

Mutation

LetA-LetB(Δ700-717, replace with 812-836)

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2116

Plasmid

#170393

Purpose

Plasmid for genetic complementation of LetAB with ΔRing 1 LetB mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Expression

Bacterial

Mutation

LetA-LetB(Δ48-159)

Promoter

T7

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2117

Plasmid

#170394

Purpose

Plasmid for genetic complementation of LetAB with ΔRing 2 LetB mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Expression

Bacterial

Mutation

LetA-LetB(Δ160-279)

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2118

Plasmid

#170395

Purpose

Plasmid for genetic complementation of LetAB with ΔRing 3 LetB mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Expression

Bacterial

Mutation

LetA-LetB(Δ280-391)

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2119

Plasmid

#170396

Purpose

Plasmid for genetic complementation of LetAB with ΔRing 4 LetB mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Expression

Bacterial

Mutation

LetA-LetB(Δ392-515)

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2120

Plasmid

#170397

Purpose

Plasmid for genetic complementation of LetAB with ΔRing 5 LetB mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Expression

Bacterial

Mutation

LetA-LetB(Δ516-633)

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2121

Plasmid

#170398

Purpose

Plasmid for genetic complementation of LetAB with ΔRing 6 LetB mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Expression

Bacterial

Mutation

LetA-LetB(Δ634-746)

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2122

Plasmid

#170399

Purpose

Plasmid for genetic complementation of LetAB with ΔRing 7 LetB mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Expression

Bacterial

Mutation

LetA-LetB(Δ747-877)

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2199

Plasmid

#170406

Purpose

Genetic complementation plasmid of LetA-[LetB PLL6->1] mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Expression

Bacterial

Mutation

LetA-LetB(Δ702-717, replace with 114-130)

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2114

Plasmid

#175961

Purpose

For expression of LetA-[ΔRing6 LetB] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

Depositor

Gira Bhabha, Damian Ekiert

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Inserts

LetA
LetB

Tags

8xHis 2xQH and TEV cleavage site on LetA

Expression

Bacterial

Mutation

Δ634-746

Promoter

araBAD

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCX-EGFP beta5 integrin receptor

Plasmid

#14996

Depositor

Raymond Birge

Article

Albert et al Nat Cell Biol. 2000 Dec . 2(12):899-905.

Insert

beta5 integrin receptor (ITGB5 Human)

Tags

IRES EGFP

Expression

Mammalian

Available Since

Aug. 22, 2007

Availability

Academic Institutions and Nonprofits only

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pET28a-His-MinD_MinE

Plasmid

#133621

Purpose

Expression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.

Depositor

Petra Schwille

Article

Loose et al Science. 2008 May 9;320(5877):789-92. do

Insert

His-MinD_MinE (minD, minE E. coli)

Expression

Bacterial

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pKB33 (pUC19-KANMX-tetO7-CYCpr)

Plasmid

#210472

Purpose

Plasmid with KanMX-tetO7-CYCpr cassette from the yTHC collection. Used for amplifying cassette with homology to regions of interest for genomic integration.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Insert

KanMX-TetO7-CYCpr cassette

Use

Synthetic Biology

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDN0606 (pDEST-DHFR F[3] N-term,HygR)

Plasmid

#210488

Purpose

pDEST vector to tag gene of interest with DHFR F[3] on the N-terminus to perform DHFR-PCA in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Expression

Yeast

Promoter

ADH1

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pKB11 (pDEST-DHFR F[1,2] C-term, NatR)

Plasmid

#210485

Purpose

pDEST vector to tag gene of interest with DHFR F[1,2] on the C-terminus to perform DHFR-PCA in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Expression

Yeast

Promoter

ADH1

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDN0605 (pDEST-DHFR F[1,2] N-term, NatR)

Plasmid

#210487

Purpose

pDEST vector to tag gene of interest with DHFR F[1,2] on the N-terminus to perform DHFR-PCA in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Expression

Yeast

Promoter

ADH1

Available Since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pKB12 (pDEST-DHFR F[3] C-term, HygR)

Plasmid

#210486

Purpose

pDEST vector to tag gene of interest with DHFR F[3] on the C-terminus to perform DHFR-PCA in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Expression

Yeast

Promoter

ADH1

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge