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Plasmid#106205PurposeMedium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.A184V
UseAAVTagsExpressionMutationGltI: A184V + mRuby3 fusionPromoterCAGAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mRuby2-MACROD1_d77
Plasmid#172588PurposeFor transient expression of C-terminal mRuby2-tagged MACROD1del1-77DepositorInsertMACROD1 (MACROD1 Human)
UseTagsmRuby2ExpressionMammalianMutationdeleted amino acids 1-76PromoterCMVAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKK-mRuby3-TEV
Plasmid#105780PurposeExpression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 26879144). mRuby3 is brighter than mCherry and has shifted ex and em spectra.DepositorTypeEmpty backboneUseFlp-in competentTagsmRuby3-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Ace2_D92N_E199V-mRuby3
Plasmid#129257PurposeExpress Ace2_D92N_E199V-mRuby3 in mammalian cellsDepositorInsertAce2_D92N_E199V-mRuby3
UseTagsExpressionMammalianMutationD92N, E199VPromoterCAGAvailable sinceAug. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-C1V1-TT-mRuby2-ST
Plasmid#109124PurposeCation channelrhodopsin C1V1 fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertC1V1-TT-mRuby2-ST
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
C1-MPAct(delta1-6)-mRuby3
Plasmid#155223PurposeF-actin bidning mutant of MPActDepositorInsertMutated version of F-tractin (aa 15-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b
UseTagsmRuby3ExpressionMammalianMutationDeletion of first 6 AA from F-tractin (9-14 from …PromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDYU1B/mRuby3–α-tubulin
Plasmid#154302PurposeExpresses mRuby3–α-tubulin from actin15 promoter in Dictyostelium discoideumDepositorInsertalpha-tubuin
UseDictyostelium expressionTagsmRuby3ExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ H2B-mRuby2
Plasmid#99270PurposeContains a fusion of human histone H2B to fluorescent protein mRuby2 in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.DepositorInsertmRuby2-3XHA
UseTagsH2BExpressionMammalianMutationPromoterSP6Available sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ChETA-mRuby2-ST
Plasmid#109126PurposeCation channelrhodopsin ChETA fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertChETA-mRuby2-ST
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-mRuby3
Plasmid#105797PurposeExpression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 26879144). mRuby3 is brighter than mCherry and has shifted ex and em spectra.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mRuby3ExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-eGtACR1-mRuby2-ST
Plasmid#109136PurposeAnion channelrhodopsin GtACR1 fused to ER export signal, mRuby2 and targeted to the neuronal soma and proximal dendritesDepositorInserteGtACR1-mRuby2-ST
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2- mRuby-Rab35
Plasmid#194338PurposeExpresses mRuby Rab35 in mammalian cells and zebrafish embryosDepositorInsertRab35 (RAB35 Human)
UseTagsmRubyExpressionMammalianMutationPromoterCMV IE94Available sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG-FLEX.SF-iGluSnFR.S72A.mRuby3
Plasmid#106206PurposeWeak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.S72A
UseAAVTagsExpressionMutationGltI: S72A + mRuby3 fusionPromoterCAGAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV- CAG-h2BmRuby2FLAG
Plasmid#116872PurposeNuclear localized mRuby2FLAGDepositorInsertH2BmRuby2FLAG
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-ccdB-mRuby3
Plasmid#166139PurposeGateway destination vector for generation of Galactose-inductibe, C-terminal mRuby3-tagged proteins in yeast. Contains a CEN/ARS element for low copy number when in yeast.DepositorArticleTypeEmpty backboneUseTagsmRuby3ExpressionYeastMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Ace2_D92N_E199V-mRuby3-ST
Plasmid#129262PurposeExpress soma-localized Ace2_D92N_E199V-mRuby3 in mammalian cellsDepositorInsertAce2_D92N_E199V-mRuby3-ST
UseTagsExpressionMammalianMutationD92N, E199VPromoterCAGAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Chronos-mRuby2-ST
Plasmid#105446PurposeCation channelrhodopsin Chronos fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertChronos-mRuby2-ST
UseTagsmRuby2 and soma targeting motifExpressionMutationPromoterpCAGGSAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
mRuby-PCNA-19-NLS-4
Plasmid#55876PurposeLocalization: Nucleus, Excitation: 558, Emission: 605DepositorInsertPCNA-19 NLS (PCNA Human)
UseTags3xNLS and mRubyExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAG-CoChR-mRuby2-ST
Plasmid#109131PurposeCation channelrhodopsin CoChR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertCoChR-mRuby2-ST
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
mRuby-Gamma-Tubulin-17
Plasmid#55864PurposeLocalization: Centrosomes, Excitation: 558, Emission: 605DepositorInsertGamma-Tubulin (TUBG1 Human)
UseTagsmRubyExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits