We narrowed to 13,037 results for: Mpl

1. pcDNA3.1-MS2-HA-HsNanos3-del4-20_X

   Plasmid

   #147945

   Purpose

   Mammalian Expression of HsNanos3-del4-20

   Depositor

   Elisa Izaurralde

   Article

   Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

   Insert

   HsNanos3-del4-20 (NANOS3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMT-EGFP-CTAP-PrP-SBP_W

   Plasmid

   #147835

   Purpose

   Insect Expression of EGFP

   Depositor

   Elisa Izaurralde

   Article

   Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-MS2-HA-HsSmg5-G120E_W

   Plasmid

   #147819

   Purpose

   Mammalian Expression of HsSmg5-G120E

   Depositor

   Elisa Izaurralde

   Article

   Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

   Insert

   HsSmg5-G120E (SMG5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one silent mutation compared to the sequence give…

   Promoter

   Available since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1-MS2-HA-HsSmg7_633-1091_T

   Plasmid

   #147558

   Purpose

   Mammalian Expression of HsSmg7_633-1091

   Depositor

   Elisa Izaurralde

   Article

   Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

   Insert

   HsSmg7_633-1091 (SMG7 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one non silent mutation V854I compared to the seq…

   Promoter

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAc5.1B-lambdaN-HA-DmGe1_944-1354-V5His6_D

   Plasmid

   #146136

   Purpose

   Insect Expression of DmGe1_944-1354

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmGe1_944-1354 (Ge-1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAc5.1B-lambdaN-HA-DmCup_C

   Plasmid

   #146064

   Purpose

   Insect Expression of DmCup

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmCup (cup Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Two silent mutations compared to the sequence giv…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAc5.1B-lambdaN-HA-DmMe31B_1-267_C

   Plasmid

   #146065

   Purpose

   Insect Expression of DmMe31B_1-267

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmMe31B_1-267 (me31B Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAc5.1B-lambdaN-HA-DmMe31B_268-459_C

   Plasmid

   #146066

   Purpose

   Insect Expression of DmMe31B_268-459

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmMe31B_268-459 (me31B Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAc5.1B-lambdaN-HA-DmHPat_A

   Plasmid

   #145893

   Purpose

   Insect Expression of DmHPat

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmHPat (Patr-1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   three silent mutations compared to the sequence g…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pOME0135

    Plasmid

    #178397

    Purpose

    KSHV Kaposincluster-RevCompl-Syn

    Depositor

    Dirk Dittmer

    Article

    Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

    Insert

    Kaposincluster-RevCompl-SYN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. L1-Ck1-hs1-l-chlo

    Plasmid

    #163937

    Purpose

    For integration in the chloroplast trnG_trnfM region: HS1-L, genome position: 40216_ 41843, Genbank accession no. MH635409

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Marchantia polymorpha trnG_trnfM region left homologous sequence
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. L1-Ck4-hs1-r-chlo

    Plasmid

    #163940

    Purpose

    For integration in the chloroplast trnG_trnfM region: HS1-R, genome position: 41847_ 43622, Genbank accession no. MH635409

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Marchantia polymorpha trnG_trnfM region right homologous sequence
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. L1-Ck4-hs2-r-chlo

    Plasmid

    #163941

    Purpose

    For integration in the chloroplast rbcL_trnR region: HS2-R, genome position: 57681- 59304, Genbank accession no. MH635409

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Marchantia polymorpha rbcL_trnR region right homologous sequence
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. PROM5_MpU6

    Plasmid

    #136120

    Purpose

    Mp U6 promoter (type III RNA polymerase promoter) to drive expression of gRNA for CRISPR/Cas9

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    PROM5_MpU6
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CDS_Cas9-NLS

    Plasmid

    #136121

    Purpose

    Puchta Arabidopsis codon-optimised Cas9 for CRISPR

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CDS_AtcoCas9-NLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CDS12-MpER-Targ

    Plasmid

    #136094

    Purpose

    CDS12 with ER targeting peptide to fuse with CTAG containing HDEL ER retention signal at the 3'end

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    ER targeting peptide from predicted chitinase Mapoly0069s0092
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. CTAG_eGFP-GHtag-HDEL

    Plasmid

    #136096

    Purpose

    FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide. Contains Gly-His tag

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CTAG_eGFP(noATG,noStop)-3xGly5xHys-HDEL
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-anticalin (E60amb)-His

    Plasmid

    #168043

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding E60 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Glu 60 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-anticalin (E143amb)-His

    Plasmid

    #168046

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding E143 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Glu 143 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-anticalin (S87amb)-His

    Plasmid

    #168044

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding S87 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Ser 87 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28a-anticalin (N116amb)-His

    Plasmid

    #168045

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding N116 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Asn 116 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28a-anticalin (N21amb)-His

    Plasmid

    #168041

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding N21 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Asn 21 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28a-Fgβ-anticalin-His-ybbr

    Plasmid

    #168040

    Purpose

    Expresses anticalin targeting CTLA-4 with Fgβ peptide at the N terminus and 6x-His tag and ybbr tag at the C terminus

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag, Fgβ peptide, and ybbr tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28a(+)-HisTim12_CtoA

    Plasmid

    #170281

    Purpose

    Expresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

    Depositor

    Paul Schanda

    Article

    Weinhaupl et al Structure. 2021 May 3. pii: S0969-2126(2

    Insert

    yeast Tim12 (TIM12 Budding Yeast)
    

    Use

    Tags

    TEV-cleavable N-terminal His tag (on backbone)

    Expression

    Bacterial

    Mutation

    mutated two non-conserved Cys to Ala

    Promoter

    Available since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28a-anticalin (I55amb)-His

    Plasmid

    #168042

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding I55 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Ile 55 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRS406 ATG1-ATG7-3'UTR

    Plasmid

    #166847

    Purpose

    Expresses Atg1 with ATG7 3'UTR in yeasts.

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Mol Cell. 2019 Jan 17;73(2):314-324.e4.

    Insert

    ATG1 (ATG1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ATG1 Promoter

    Available since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pENTR-D-TOPO-kcnt2a

    Plasmid

    #164963

    Purpose

    Zebrafish kcnt2a gene CDS

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

    Insert

    kcnt2a (kcnt2 Zebrafish)
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28a-Coh-ddFLN4-ELP(MV7E2)3-His-ybbR

    Plasmid

    #157672

    Purpose

    Expression of Coh (type III from Ruminococcus flavefaciens) with ddFLN4, ELP linker, His6x tag, and ybbR Tag.

    Depositor

    Michael Nash

    Article

    Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

    Insert

    Cohesin (R.f) with ddFLN4, ELP, Histag and ybbR tag
    

    Use

    Tags

    ddFLN4, ELP, Histag and ybbR tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFA-DAB1

    Plasmid

    #162691

    Purpose

    Expresses DabAB1 inorganic carbon transport complex

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    DabAB1 inorganic carbon transport complex
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCFJ1972 - [1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)

    Plasmid

    #159844

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCFJ2301 - [1-2] ENTR - Fluor - ce-mMaple3(1 intron, syntrons(3), no_atg, no_stop)

    Plasmid

    #159865

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-mMaple3(1 intron, syntrons(3), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSWD95Y14D

    Plasmid

    #159098

    Purpose

    CckA FRET Sensor with cyclic-di-GMP point mutant

    Depositor

    Seth Childers

    Article

    Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

    Insert

    CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691(Y514D), Linker SNVTRHRSAT, mRuby3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Mutated Y514 to D514

    Promoter

    Xylose

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSWD95D293A

    Plasmid

    #159099

    Purpose

    CckA FRET Sensor with DITE motif mutant

    Depositor

    Seth Childers

    Article

    Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

    Insert

    CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691 (D293A), Linker SNVTRHRSAT, mRuby3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    CckA D293 mutated to A293 and V199I on mRuby3 (P…

    Promoter

    Xylose

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET_4E2H.210

    Plasmid

    #155212

    Purpose

    Expresses 4E2H.210 de novo protein in E.coli

    Depositor

    Bruno Correia

    Article

    Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

    Insert

    4E2H.210
    

    Use

    Tags

    6x His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEBTetBl-CLIP-Centrin3

    Plasmid

    #136855

    Purpose

    Mammalian expression of the centrosomal protein Centrin3 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-Centrin3
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEBTetDBl-CLIP-Centrin3

    Plasmid

    #136856

    Purpose

    Mammalian expression of the centrosomal protein Centrin3 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-Centrin3
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEBTetDBl-CLIP-FOP

    Plasmid

    #136846

    Purpose

    Mammalian expression of the centrosomal protein FGFR N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-FGFR
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEBTetDBl-CLIP-POC5

    Plasmid

    #136836

    Purpose

    Mammalian expression of the centrosomal protein POC5 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-POC5 (POC5 Human, Synthetic)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pEBTetBl-CLIP-FOP

    Plasmid

    #136845

    Purpose

    Mammalian expression of the centrosomal protein FGFR N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-FGFR
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEBTetBl-CLIP-POC5

    Plasmid

    #136835

    Purpose

    Mammalian expression of the centrosomal protein POC5 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-POC5 (POC5 Human, Synthetic)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pEBTetD-SNAP-POC5

    Plasmid

    #136787

    Purpose

    Mammalian expression of the centrosomal protein POC5 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-POC5 (POC5 Human, Synthetic)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCool-human TSC1 (939-992)

    Plasmid

    #132618

    Purpose

    expresses human TSC1 (939-992) in E. coli

    Depositor

    Wenqing Xu

    Article

    Qin et al J Biol Chem. 2016 Apr 15;291(16):8591-60

    Insert

    TSC1 (939-992)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. K8.1Pr-pGL4.16-OriLyt

    Plasmid

    #131038

    Purpose

    Firefly Luciferase reporter for K8.1 promoter from KSHV with the lytic origin of replication

    Depositor

    Britt Glaunsinger

    Article

    Nandakumar et al PLoS Pathog. 2019 May 16;15(5):e1007774.

    Insert

    KSHV Lytic Origin of replication
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pET15b-GBdm

    Plasmid

    #129690

    Purpose

    express E. coli GreB E82C/C68S

    Depositor

    Jeff Gelles

    Article

    Tetone et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

    Insert

    GreB E82C C68S (greB E. coli)
    

    Use

    Tags

    his6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pGBDU-Atg17(F317D, I325A)

    Plasmid

    #122275

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Phenylalanine 317 to Aspartic acid; Isoleucine 32…

    Promoter

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pGAD-Atg17(F317H)

    Plasmid

    #122269

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Phenylalanine 317 to Histidine

    Promoter

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pGBDU-Atg17(I325A)

    Plasmid

    #122274

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Isoleucine 325 to alanine

    Promoter

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pAPM-D4 miR30-PIWIL2 ts2

    Plasmid

    #115870

    Purpose

    PIWIL2 knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    PIWIL2 shRNA (PIWIL2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pGAD-Atg17(F317D, I325A)

    Plasmid

    #122273

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Phenylalanine 317 to Aspartic acid; Isoleucine 32…

    Promoter

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pGAD-Atg17(I325A)

    Plasmid

    #122272

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Isoleucine 325 to alanine

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge