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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 42,575 results for: cha

Showing: 2181 - 2200 of 42575 results

  3. pKP134 [pRS406-YBR139Wp-YBR139W(S219,D415,H474A)-GFP-ADH1t]

    Plasmid
    #106469
    Purpose
    Expresses Atg42/Ybr139w(S219,D415,H474A) with a C-terminal GFP tag in yeast cells
    Depositor
    Daniel Klionsky
    Article
    Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109
    Insert
    YBR139W(S219,D415,H474A)
    Use
    Tags
    GFP
    Expression
    Bacterial and Yeast
    Mutation
    Changed Serine 219, Aspartate 415 and Histidine 4…
    Promoter
    YBR139W
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    106469-plasmid-map-sequence-id-204903

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  5. CPH3568 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,N1501K; msLink1)

    Plasmid
    #91819
    Purpose
    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD
    Depositor
    Chris Hill
    Article
    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1
    Insert
    RPO21 (RPO21 Budding Yeast)
    Use
    Tags
    10xHis
    Expression
    Yeast
    Mutation
    5xGly linker, PreScission Protease site (LEVLFQGP…
    Promoter
    Endogenous Rpb1 promoter
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    91819-plasmid-map-sequence-id-176494

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  6. pNLF1-C_PLCG1:1-1291

    Plasmid
    #211089
    Purpose
    Mammalian expression of full-length human PLCG1 isoform 2. C-terminal NanoLuc luciferase tag.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    PLCG1:M1-L1291 (PLCG1 Human)
    Use
    Tags
    NanoLuc luciferase
    Expression
    Mammalian
    Mutation
    isoform 2. I813T natural variant (VAR_011908)
    Promoter
    CMV
    Available since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211089-plasmid-map-sequence-id-425967

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  15. pFBOH-MHL_EML4:250-877

    Plasmid
    #210914
    Purpose
    Insect expression of human EML4 fragment I250 to A877. N-terminal 6X His tag with TEV cleavage.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    EML4:I250-A877 (EML4 Human)
    Use
    Tags
    MHHHHHHSSGRENLYFQG
    Expression
    Insect
    Mutation
    K283E natural variant (VAR_031726), I382V Natural…
    Promoter
    Polyhedrin
    Available since
    March 19, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210914-plasmid-map-sequence-id-426151

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  17. pFBOH-MHL_LRRK2:2141-2527

    Plasmid
    #210899
    Purpose
    Insect expression of human LRRK2 fragment T2141 to E2527. N-terminal 6X His tag with TEV cleavage.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    LRRK2:T2141-E2527 (LRRK2 Human)
    Use
    Tags
    MHHHHHHSSGRENLYFQG
    Expression
    Insect
    Mutation
    M2397T natural variant ( VAR_024965)
    Promoter
    Polyhedrin
    Available since
    March 19, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210899-plasmid-map-sequence-id-426130

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Showing: 2181 - 2200 of 42575 results