Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 5,211 results for: golden gate

Showing: 2181 - 2200 of 5211 results

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LLP1009

Plasmid

#239931

Purpose

sgRNA compatible with TCTP-SynPro-10 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Publication

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP1010

Plasmid

#239932

Purpose

dCas9 with Stop codon as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Publication

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

dCas9

Use

CRISPR and Synthetic Biology

Mutation

N/A

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pNS1D-sgRNA9-SpR

Plasmid

#191092

Purpose

aTc inducible gRNA expression plasmid with BbsI sites for Golden Gate insertion of crRNA oligos and homology arms for genome integration at NS1D in Synechococcus sp. PCC 7002

Depositor

Anne Ruffing

Publication

Dallo et al ACS Synth Biol. 2023 Apr 21;12(4):1175-1

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

tet

Available since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pGA-red-mini

Plasmid

#196338

Purpose

Easy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screening

Depositor

Paola Branduardi

Publication

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology; Golden gate assembly backbone

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pUC57-mini v2-HF24_F4

Plasmid

#195718

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Publication

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#4 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available since

March 20, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F20

Plasmid

#195734

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Publication

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#20 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available since

March 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F5

Plasmid

#195719

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Publication

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#5 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available since

March 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F12

Plasmid

#195726

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Publication

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#12 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available since

March 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F3

Plasmid

#195717

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Publication

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#3 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available since

March 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F24

Plasmid

#195738

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Publication

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#24 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available since

March 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F7

Plasmid

#195721

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Publication

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#7 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available since

March 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F8

Plasmid

#195722

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Publication

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#8 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available since

March 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F14

Plasmid

#195728

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Publication

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#14 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available since

March 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F13

Plasmid

#195727

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Publication

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#13 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F22

Plasmid

#195736

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Publication

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#22 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F10

Plasmid

#195724

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Publication

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#10 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F6

Plasmid

#195720

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Publication

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#6 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F15

Plasmid

#195729

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Publication

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#15 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F17

Plasmid

#195731

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Publication

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#17 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F19

Plasmid

#195733

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Publication

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#19 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge

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