We narrowed to 5,457 results for: golden gate
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Plasmid#184887PurposeABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA deliveryDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterRPS5AAvailable sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only
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pLSL.R.Ly
Plasmid#227697PurposeReengineered geminiviral replicon vectorDepositorTypeEmpty backboneExpressionPlantAvailable sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ142-ZmUbi
Plasmid#138106PurposeGolden Gate recipient and Gateway entry vector; assembly of 2 gRNAs driven by Zea mays Ubi promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ144-ZmUbi-pT
Plasmid#138108PurposeGolden Gate recipient and Gateway entry vector; assembly of 4 gRNAs driven by Zea mays Ubi promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNP3425 [pNP2922 - ISCro4 Enhanced bRNA (TBL4, WT + DBL3, WT) + ISCro4 Recombinase (WT)]
Plasmid#247262PurposeExpress ISCro4 recombinase and bridge RNADepositorInsertpNP2922 - ISCro4 bRNA1-179 C60U, A61G, U73C, dU86, A87G, dU88, ins179(rc(101-111) (TBL4+DBL3)
ExpressionMammalianMutationC60U, A61G, U73C, dU86, A87G, dU88, ins179(rc(101…Available sinceOct. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNP3614-MM168 [pNP3613 - ISCro4 Recombinase (S30T/P54Q/S243H)]
Plasmid#247266PurposeExpress ISCro4 recombinaseDepositorInsertpNP3613 - ISCro4 Recombinase (S30T/P54Q/S243H)
ExpressionMammalianMutationS30T/P54Q/S243HAvailable sinceOct. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
PX458 eSp(1.1) V3
Plasmid#226963PurposeCBh-eSpCas9(1.1)-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132B-TLS-CsALSgR1.1
Plasmid#245877PurposeGolden gate entry vector carrying the 1st gRNA for base editing in Carrizo citrange ALS gene along with TLS mobile RNA sequenceDepositorInsertCsALSgR1.1
UseCRISPRExpressionPlantPromoterAtU3Available sinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133B-TLS-CsALSgR1.2
Plasmid#245878PurposeGolden gate entry vector carrying the 2nd gRNA for base editing in Carrizo citrange ALS gene along with TLS mobile RNA sequenceDepositorInsertCsALSgR1.2
UseCRISPR; Golden gate entry vectorExpressionPlantPromoterAtU3Available sinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132B-TLS-PtALSgRNA
Plasmid#245881PurposeGolden gate entry vector carrying the gRNA for base editing in Poplar hybrid ALS gene along with TLS mobile RNA sequenceDepositorInsertPtALSgRNA
UseCRISPRExpressionPlantPromoterAtU3Available sinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132B-CsALSgR1.1
Plasmid#245875PurposeGolden gate entry vector carrying the 1st gRNA for base editing in Carrizo citrange ALS geneDepositorInsertCsALS_gRNA1.1
UseCRISPR; Golden gate entry vector to expressing t…ExpressionPlantPromoterAtU3Available sinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133B-CsALSgR1.2
Plasmid#245876PurposeGolden gate entry vector carrying the 2nd gRNA for base editing in Carrizo citrange ALS geneDepositorInsertCsALSgR1.2
UseCRISPR; Golden gate entry vectorExpressionPlantPromoterAtU3Available sinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCAV035_mCherry_P2A_fsEGFP_GGsite
Plasmid#219807PurposeFluorescent Reporter for MUTYH-Specific Activity; Containing BsaI Recognition Sequence at Frameshifted EGFP; For OG:A Oligo Insertion at Golden Gate (GG) Site at Codon 34DepositorInsertmCherry
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pLenti-sgStuffer-GFP-Puro
Plasmid#208349PurposeLentivirus sgRNA backbone with GFP and PuroR. Compatible with golden gate cloning (Esp3i/BsmBI)DepositorPublicationTypeEmpty backboneUseCRISPR and LentiviralPromoterhU6Available sinceJan. 29, 2026AvailabilityAcademic Institutions and Nonprofits only -
pLenti-sgStuffer-mCherry-Puro
Plasmid#208350PurposeLentivirus sgRNA backbone with mCherry and PuroR. Compatible with golden gate cloning (Esp3i/BsmBI)DepositorPublicationTypeEmpty backboneUseCRISPR and LentiviralPromoterhU6Available sinceJan. 29, 2026AvailabilityAcademic Institutions and Nonprofits only -
LLP1003
Plasmid#239925PurposesgRNA compatible with TCTP-SynPro-07 and CaMV 35S-SynPro-08 as Level-0 part (Golden Gate cloning)DepositorInsertsgRNA
UseSynthetic BiologyMutationN/AAvailable sinceJan. 28, 2026AvailabilityAcademic Institutions and Nonprofits only -
pMK024
Plasmid#236571PurposeCloning vector for PaqCI Golden Gate assembly of ISAam1 guides into ISAam1 plant expression vectorDepositorTypeEmpty backboneExpressionPlantAvailable sinceOct. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKMW121_WT-Cas9_U6-entry
Plasmid#244818PurposeMammalian expression of WT Cas9 with Golden Gate-compatible sgRNA spacer cassetteDepositorInsertSpyCas9 (WT)
UseCRISPRTags3xFLAG-SV40 NLS and Nucleoplasmin NLSExpressionMammalianPromoterCAG, U6Available sinceSept. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKMW493_U6-sgRNA-entry
Plasmid#244823PurposeMammalian expression of SpyCas9 single guide RNA with Golden Gate-compatible sgRNA spacerDepositorInsertSpyCas9 single guide RNA
UseCRISPRExpressionMammalianPromoterU6Available sinceSept. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
LLP1011
Plasmid#239933PurposedCas9 without Stop codon as Level-0 part (Golden Gate cloning)DepositorInsertdCas9
UseCRISPR and Synthetic BiologyMutationN/AAvailable sinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only