We narrowed to 15,088 results for: pET

1. pET28-p53 (1-73) (72P)

   Plasmid

   #62083

   Purpose

   Expression of human p53 (1-73) (72P) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

   Insert

   p53 (TP53 Human)
   

   Tags

   HIs

   Expression

   Bacterial

   Mutation

   contains TAD residues 1-73; 72P

   Promoter

   T7

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pET28-MRPP3-His

   Plasmid

   #67866

   Purpose

   bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. His tag

   Depositor

   Walter Rossmanith

   Article

   Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583

   Insert

   MRPP3 (KIAA0391 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Amino acid 46 of recombinant MRPP3 is preceded by…

   Promoter

   T7

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28b(+)-SPN+IFS

   Plasmid

   #83372

   Purpose

   Expresses SPN and IFS complex in E. coli cell

   Depositor

   SeWon Suh

   Article

   Yoon et al J Synchrotron Radiat. 2013 Nov;20(Pt 6):

   Inserts

   SPN
   IFS
   

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFS_0160-pET30-Fusicatenibacter_saccharivorans_ARRAY2

   Plasmid

   #116953

   Purpose

   Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with classic acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Fusicatenibacter saccharivorans RT-Cas1-Cas2
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET20b-GpNLuc-6xHis

   Plasmid

   #135932

   Purpose

   Expresses the GpNLuc LumiFluor in bacterial cells.

   Depositor

   Antonio Amelio

   Article

   Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

   Insert

   GpNLuc LumiFluor
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28_6xHis-PARP1delWGR-L713F

   Plasmid

   #173941

   Purpose

   Bacterial expression of PARP1 lacking WGR domain but containing L713F gain-of-function mutation

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delWGR-L713F (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 521-660; L713F, V762A

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21-pelB-LaG-27

   Plasmid

   #172752

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-27
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET3-ARL15-His6

   Plasmid

   #201880

   Purpose

   Used for bacterial expression of human protein ARL15

   Depositor

   Richard Kahn

   Insert

   ARL15 (ARL15 Human)
   

   Tags

   6xHis (GSGSGSHHHHHH)

   Expression

   Bacterial

   Available Since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a_6HT-TBP6.7(R49A)

   Plasmid

   #112726

   Purpose

   Arg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(R49A)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   R49A

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. eGFP-Silicatein-pET28

    Plasmid

    #198012

    Purpose

    C-terminal eGFP fusion to truncated silicatein insert with N- and C-terminal hexahistidine tag for purification

    Depositor

    Bryan Berger

    Article

    Vigil et al Materials Advances 2, 2023

    Insert

    eGFP-silicatein
    

    Tags

    hexahistidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-PesaR-Clux

    Plasmid

    #47658

    Purpose

    PesaR-C promoter, luxCDABE, and terminator in pET17b(AmpR)

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Synth Biol. 2013 Jul 23.

    Insert

    PesaR-C
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Additional esa box between -35 site and the origi…

    Promoter

    PesaR-C

    Available Since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pETM33_ZYMND11_coiled-coil MYND

    Plasmid

    #178493

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    ZYMND11_coiled-coil MYND (ZMYND11 Human, Synthetic)
    

    Tags

    His-GST

    Expression

    Bacterial

    Promoter

    T7/LacO

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET3a-UBE2J1 1-283

    Plasmid

    #185348

    Purpose

    In vitro transcription and translation in bacterial system

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    UBE2J1 (UBE2J1 Human)
    

    Expression

    Bacterial

    Mutation

    Cytoplasmic domain only, aa 1-283

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. HOXD9 (human) pET

    Plasmid

    #8570

    Depositor

    Corey Largman

    Insert

    HOXD9 (HOXD9 Human)
    

    Tags

    T7

    Expression

    Bacterial

    Mutation

    PCR human cDNA clone; makes protein by in vitro T…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-HJURP-CBP

    Plasmid

    #30393

    Depositor

    Yawen Bai

    Article

    Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

    Insert

    HJURP CenpA Binding Domain (CENPA Human, Budding Yeast)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    July 11, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pET30a-SRAP-delCys2

    Plasmid

    #84568

    Purpose

    Encodes murine SRAP domain (250aa) of Hmces/Srap1 with deletion of catalytic Cys2

    Depositor

    Douglas Feldman

    Article

    Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

    Insert

    Srap1 (Hmces Mouse)
    

    Tags

    6xHis and S-Tag

    Expression

    Bacterial

    Mutation

    deletion of Cys2

    Promoter

    T7

    Available Since

    Nov. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET20b-delta133HtrA2 A141S

    Plasmid

    #16157

    Depositor

    L. Miguel Martins

    Article

    Strauss et al Hum Mol Genet. 2005 Aug 1. 14(15):2099-1

    Insert

    HtrA2 (HTRA2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    A141S

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. CyPet-A206K-PRL2

    Plasmid

    #149685

    Purpose

    For the FRET-based binding assay between human CNNM3 CBS domain and PRL2.

    Depositor

    Motoyuki Hattori

    Article

    Cai et al Sci Rep. 2020 Jul 30;10(1):12879. doi: 1

    Insert

    CyPet-A206K-PRL2
    

    Tags

    8 histidine tag

    Expression

    Bacterial

    Mutation

    CyPet A206K

    Promoter

    T7

    Available Since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28 Set9 mutant

    Plasmid

    #24083

    Depositor

    Danny Reinberg

    Article

    Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

    Insert

    Set9 (SETD7 Human)
    

    Tags

    FLAG and His

    Expression

    Bacterial

    Mutation

    H297A

    Available Since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pET-28a(+)-FPPS

    Plasmid

    #108952

    Purpose

    heterologous expression of FPPS of G. stearothermophilus in E. coli

    Depositor

    Frank Schulz

    Article

    Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

    Insert

    Farnesyldiphosphat synthase (FPPS)
    

    Tags

    His6-Tag

    Expression

    Bacterial

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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