We narrowed to 14,580 results for: pET

1. pET21-pelB-LaG-2

   Plasmid

   #172739

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-2; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-2
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-PTBP1-RRM12CCtoSS

   Plasmid

   #89155

   Purpose

   Recombinant expression of PTBP1-RRM12 C250S,C251S in E.coli

   Depositor

   Frédéric Allain

   Article

   Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

   Insert

   Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1+2 mutant C250S, C251S (PTBP1 Human)
   

   Use

   Tags

   hexa His

   Expression

   Bacterial

   Mutation

   mutations: Cys 250 to Ser, Cys 251 to Ser

   Promoter

   T7

   Available since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-ClbN-NHis

   Plasmid

   #51497

   Purpose

   NRPS module containing CATE domains with N-terminal 6His tag

   Depositor

   Emily Balskus

   Article

   Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

   Insert

   ClbN
   

   Use

   Tags

   6x His tag and T7 tag

   Expression

   Bacterial

   Mutation

   contains aa30-1094 of NP_754347.1

   Promoter

   T7

   Available since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-PesaR-Plux

   Plasmid

   #47659

   Purpose

   PesaR-P promoter, luxCDABE, and terminator in pET17b (AmpR)

   Depositor

   Cynthia Collins

   Article

   Shong et al ACS Synth Biol. 2013 Jul 23.

   Insert

   PesaR-P
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Additional esa box downstream of the original esa…

   Promoter

   PesaR-P

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pET - FnR062.Y78W

   Plasmid

   #84802

   Purpose

   bacterial expression of FnR.0.6.2-Y78W protein

   Depositor

   Benjamin Hackel

   Article

   Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

   Insert

   FnR.0.6.2-Y78W
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Y78W

   Promoter

   Available since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

6. pET - FnR062.Y78G

   Plasmid

   #84789

   Purpose

   bacterial expression of FnR.0.6.2-Y78G protein

   Depositor

   Benjamin Hackel

   Article

   Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

   Insert

   FnR.0.6.2-Y78G
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Y78G

   Promoter

   Available since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

7. pET - FnR062.Y78H

   Plasmid

   #84790

   Purpose

   bacterial expression of FnR.0.6.2-Y78H protein

   Depositor

   Benjamin Hackel

   Article

   Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

   Insert

   FnR.0.6.2-Y78H
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Y78H

   Promoter

   Available since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

8. pET - FnR062.Y78I

   Plasmid

   #84791

   Purpose

   bacterial expression of FnR.0.6.2-Y78I protein

   Depositor

   Benjamin Hackel

   Article

   Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

   Insert

   FnR.0.6.2-Y78I
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Y78I

   Promoter

   Available since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

9. pET - FnR062.Y78P

   Plasmid

   #84796

   Purpose

   bacterial expression of FnR.0.6.2-Y78P protein

   Depositor

   Benjamin Hackel

   Article

   Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

   Insert

   FnR.0.6.2-Y78P
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Y78P

   Promoter

   Available since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

10. pET - FnR062.Y78R

    Plasmid

    #84798

    Purpose

    bacterial expression of FnR.0.6.2-Y78R protein

    Depositor

    Benjamin Hackel

    Article

    Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

    Insert

    FnR.0.6.2-Y78R
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y78R

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

11. pET - FnR062.Y78S

    Plasmid

    #84799

    Purpose

    bacterial expression of FnR.0.6.2-Y78S protein

    Depositor

    Benjamin Hackel

    Article

    Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

    Insert

    FnR.0.6.2-Y78S
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y78S

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

12. pET - FnR062.Y78D

    Plasmid

    #84786

    Purpose

    bacterial expression of FnR.0.6.2-Y78D protein

    Depositor

    Benjamin Hackel

    Article

    Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

    Insert

    FnR.0.6.2-Y78D
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y78D

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

13. pET - FnR062.Y78F

    Plasmid

    #84788

    Purpose

    bacterial expression of FnR.0.6.2-Y78F protein

    Depositor

    Benjamin Hackel

    Article

    Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

    Insert

    FnR.0.6.2-Y78F
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y78F

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

14. pET - FnR062.Y78

    Plasmid

    #84783

    Purpose

    bacterial expression of FnR.0.6.2-Y78 protein

    Depositor

    Benjamin Hackel

    Article

    Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

    Insert

    FnR.0.6.2-Y78
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pET41s-GST-ATF6a-AD

    Plasmid

    #64774

    Purpose

    bacterial expression of GST-tagged ATF6 (1-150)

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    ATF6a-AD (1-150) (ATF6 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    C-term deletion

    Promoter

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pET28-p53 (1-73) (72R)

    Plasmid

    #62082

    Purpose

    Expression of human p53 (1-73) (72R) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    HIs

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-73; 72R

    Promoter

    T7

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pET11a-p52(1-398)

    Plasmid

    #44733

    Depositor

    Gourisankar Ghosh

    Insert

    p52 (NFKB2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    AA 1-398

    Promoter

    T7

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pET28a-Hook3 aa 1-239

    Plasmid

    #74609

    Purpose

    Expresses Hook domain and first coiled coil of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239 (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28b+R3(R112C/T180C)

    Plasmid

    #108195

    Purpose

    Expresses PdR-PCNA3(R112C/T180C) fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PdR-PCNA3(R112C/T180C) fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C/T180C(PCNA3)

    Promoter

    T7 promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pETO18G-H7C/R86C

    Plasmid

    #27403

    Depositor

    Jonathan Javitch

    Article

    Zhao et al Nature. 2010 May 13. 465(7295):188-93.

    Insert

    leuT
    

    Use

    Tags

    HisX8 and Thrombin

    Expression

    Bacterial

    Mutation

    Changes His 7 to Cys and Arg 86 to Cys

    Promoter

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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