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pHAGE-TO-dCas9-3XGFP

Plasmid

#64107

Purpose

Expression of Sp dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Promoter

CMV-TO

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pEF SYT1-HaloTag

Plasmid

#197281

Purpose

Encodes full-length wild type synaptotagmin 1 (SYT1) with a C-terminal HaloTag

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

SYT1 and HaloTag (Syt1 Rat)

Expression

Mammalian

Mutation

N/A

Promoter

EF1alpha

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pBAMD1-4

Plasmid

#61565

Purpose

Standardized mini-Tn5 delivery plasmid, streptomycin/spectinomycin resistant

Depositor

Víctor de Lorenzo

Article

Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

Type

Empty backbone

Use

Synthetic Biology; Mini-tn5 vector

Available Since

Jan. 7, 2015

Availability

Academic Institutions and Nonprofits only

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CSII-prEF1a-mCherry-3xNLS

Plasmid

#125262

Purpose

Fluorescent reporter for cell size

Depositor

Jan Skotheim

Article

Berenson et al Mol Biol Cell. 2019 Nov 15;30(24):2985-2

Insert

mCherry-3xNLS

Use

Lentiviral

Tags

3xNLS

Expression

Mammalian

Promoter

EF1a

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-dCas9-3XmCherry

Plasmid

#64108

Purpose

Expression of Sp dCas9-3XmCherry in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

CRISPR and Lentiviral

Tags

3XmCherry

Expression

Mammalian

Promoter

CMV-TO

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-shRNA-Tet1

Plasmid

#85742

Purpose

shRNA against mouse Tet1

Depositor

Hongjun Song

Article

Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

Insert

shRNA Tet1

Use

AAV

Tags

EYFP

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1

Plasmid

#221484

Purpose

NKX2-1 short isoform lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-RBM3

Plasmid

#87860

Purpose

Expresses human RBM3 coupled to GFP

Depositor

Maria Alvarado-Kristensson

Article

Rossello et al Heliyon. 2016 Sep 24;2(9):e00166. eColle

Insert

RNA binding motif protein 3 (RBM3 Human)

Tags

GFP

Expression

Mammalian

Mutation

NA

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO-puro FLAG SREBP1

Plasmid

#32017

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

SREBP1 (Srebf1 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

V225M

Promoter

CMV

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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EC70202

Plasmid

#209463

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas9 sgRNA

Use

CRISPR

Promoter

TaU6

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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