We narrowed to 3,920 results for: pCMV mammalian

1. pCMV-T7-hAcrVA3-NLS(sv40) (BPK5077)

   Plasmid

   #115140

   Purpose

   CMV-T7 promoter expression plasmid for human codon optimized AcrVA3 anti-CRISPR protein with C-terminal NLS

   Depositor

   Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

   Article

   Marino et al Science. 2018 Sep 6. pii: science.aau517

   Insert

   human codon optimized AcrVA3 anti-CRISPR protein
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-T7-hAcrVA2-NLS(sv40) (AAS2283)

   Plasmid

   #115138

   Purpose

   CMV-T7 promoter expression plasmid for human codon optimized AcrVA2 anti-CRISPR protein with C-terminal NLS

   Depositor

   Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

   Article

   Marino et al Science. 2018 Sep 6. pii: science.aau517

   Insert

   human codon optimized AcrVA2 anti-CRISPR protein
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV5 Flag p38 alpha(agf)

   Plasmid

   #20787

   Depositor

   Roger Davis

   Article

   Raingeaud et al J Biol Chem. 1995 Mar 31. 270(13):7420-6

   Insert

   pCMV5 Flag p38 alpha (agf) (Mapk14 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   replaced Thr180 and Tyr182 with Ala and Phe, resp…

   Promoter

   Available since

   April 14, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pCMV-eA3A-BE5-(NG)-PX458-EGFP

   Plasmid

   #229687

   Purpose

   CRISPR CBE plasmid (C to T edits): eA3A-BE5-NG engineered to include the sgRNA cloning backbone from PX458 and EGFP. Includes BbsI sites for sgRNA cloning downstream of U6 promoter.

   Depositor

   Deborah Marsh

   Article

   Dickson et al Hum Mol Genet. 2023 Jun 27:ddad105. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV Flag-LRRK2 K17A/K18A

   Plasmid

   #186012

   Purpose

   Flag-LRRK2 K17A/K18A

   Depositor

   Suzanne Pfeffer

   Article

   Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

   Insert

   Flag-LRRK2 K17A/K18A (LRRK2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-hOrf2mor-NLS(sv40) (BPK5095)

   Plasmid

   #115141

   Purpose

   CMV-T7 promoter expression plasmid for human codon optimized Orf2mor anti-CRISPR protein with C-terminal NLS

   Depositor

   Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

   Article

   Marino et al Science. 2018 Sep 6. pii: science.aau517

   Insert

   human codon optimized Orf2mor anti-CRISPR protein
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)

   Plasmid

   #140002

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

   Promoter

   CMV and T7

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-T7-ABEmax(7.10)-SpCas9-NG-P2A-EGFP (RTW4564)

   Plasmid

   #140005

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized ABEmax(7.10) SpCas9-NG with P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…

   Promoter

   CMV and T7

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMVd1-NLS-GAL4-iLight-VP16-T2A-mTagBFP2

   Plasmid

   #170273

   Purpose

   NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

   Depositor

   Vladislav Verkhusha

   Article

   Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

   Insert

   NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See Comments

   Promoter

   CMVd1

   Available since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV5D HA-Δ37, 115-133, 204-217 PPM1H

    Plasmid

    #207344

    Purpose

    Mammalian expression of HA-Δ37, 115-133, 204-217 PPM1H

    Depositor

    Suzanne Pfeffer

    Article

    Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

    Insert

    PPM1H (PPM1H Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Δ37, Δ115-133, Δ204-217

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. p169 pCMV/betaAc-EGFP/beta-gal

    Plasmid

    #8388

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    floxed EGFP beta-gal
    

    Use

    Cre/Lox; Beta-gal

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

12. Polymerase expression - pCMV-ePhi29 (LM2802)

    Plasmid

    #208967

    Purpose

    Unfused ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    ePhi29-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-Flag-YAP-S163/164A

    Plasmid

    #33089

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    YAP (YAP1 )
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S163A, S164A

    Promoter

    CMV

    Available since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pCMV superYFP-AURKA K162M-mTurq2

    Plasmid

    #157773

    Purpose

    Expression of AuroraA kinase-dead biosensor under CMV promoter

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    mTurquoise2 and superYFP

    Expression

    Mammalian

    Mutation

    AURKA K162M is a kinase-dead version of AURKA

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV6-AN-DDK_WT RAD23A_delta aa 111-136

    Plasmid

    #201466

    Purpose

    Expresses a mutant form of human RAD23A lacking residues 111-136. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lacks residues 111-136

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV6-AN-DDK_WT RAD23A_delta aa 137-160

    Plasmid

    #201467

    Purpose

    Expresses a mutant form of human RAD23A lacking residues 137-160. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lacks residues 137-160

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV6-AN-DDK_WT RAD23A_delta aa 202-230

    Plasmid

    #201468

    Purpose

    Expresses a mutant form of human RAD23A lacking residues 202-230. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lacks residues 202-230

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV6-AN-DDK_WT RAD23A_delta aa 287-316

    Plasmid

    #201469

    Purpose

    Expresses a mutant form of human RAD23A lacking residues 287-316. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lacks residues 287-316

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV ShadowG-AURKA K162M-mTurq2

    Plasmid

    #157769

    Purpose

    Expression of AuroraA kinase-dead biosensor under CMV promoter

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    mTurquoise2 and shadowG

    Expression

    Mammalian

    Mutation

    AURKA K162M is a kinase-dead version of AURKA

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV ShadowY-AURKA K162M-mTurq2

    Plasmid

    #157771

    Purpose

    Expression of AuroraA kinase-dead biosensor under CMV promoter

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    mTurquoise2 and shadowY

    Expression

    Mammalian

    Mutation

    AURKA K162M is a kinase-dead version of AURKA

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge