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Showing: 4401 - 4440 of 10096 results

  3. miniSOG-Farnesyl-5

    Plasmid
    #62782
    Purpose
    Localization: Membrane, Excitation:448 / 473, Emission:500 / 528
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Use
    Tags
    miniSOG
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    62782-plasmid-map-sequence-id-254842

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  4. R777-E101 Hs.HRAS

    Plasmid
    #70385
    Purpose
    Gateway ORF clone of human HRAS [NM_005343.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    HRAS (HRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70385-plasmid-map-sequence-id-125834

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  5. pBABEpuro-H-RAS_V12

    Plasmid
    #39526
    Depositor
    Julian Downward
    Insert
    HRAS V12 (HRAS Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    V12 = G12V
    Promoter
    Available since
    Aug. 15, 2012
    Availability
    Academic Institutions and Nonprofits only

  6. mKO2-Farnesyl-5

    Plasmid
    #57904
    Purpose
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Use
    Tags
    mKO2
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. PB-Neo-TetO-Cdk12_Nterm_FlagHa

    Plasmid
    #127179
    Purpose
    N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vector
    Depositor
    Phil Sharp
    Article
    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:
    Insert
    N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    Use
    Piggybac transposase vector
    Tags
    Flag- HA- Tandem Epitopes
    Expression
    Mammalian
    Mutation
    Promoter
    TetO Promoter
    Available since
    July 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    127179-plasmid-map-sequence-id-247939

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  10. pET24a-VHH-2xTS

    Plasmid
    #109419
    Purpose
    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115
    Insert
    anti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
    Use
    Tags
    BAP, HA, His6, T7, and Tyrosine sulfation (TS) se…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    109419-plasmid-map-sequence-id-211590

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  17. pCMV6-AN-HA_aa 1-542 POLK

    Plasmid
    #201585
    Purpose
    Expresses the N-terminal clasp domain and catalytic domain (aa 1-542) of human DNA polymerase kappa with a HA tag in mammalian cells
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1
    Insert
    Polymerase kappa (POLK Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Expresses amino acids 1-542 of DNA polymerase kap…
    Promoter
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201585-plasmid-map-sequence-id-395030

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  18. iEscSnFR

    Plasmid
    #182807
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do
    Insert
    iEscSnFR
    Use
    Tags
    6xHis, HA and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    182807-plasmid-map-sequence-id-358170

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  19. iFluoxSnFR

    Plasmid
    #182808
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do
    Insert
    iFluoxSnFR
    Use
    Tags
    6xHis, HA and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    182808-plasmid-map-sequence-id-358173

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  20. PWPXLD-EGFR-P667A

    Plasmid
    #133750
    Purpose
    EGFR with mutation at proline 667 converted to alanine and tagged with HA cloned into PWPXLD plasmid
    Depositor
    Chay Kuo
    Article
    Abdi et al Cell Rep. 2019 Aug 20;28(8):2012-2022.e4
    Insert
    Mutant P667A EGFR-HA (EGFR Human)
    Use
    Lentiviral
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    proline 667 converted to alanine (Please see depo…
    Promoter
    EF1-alpha
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    133750-plasmid-map-sequence-id-264885

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  21. iSKetSnFR2

    Plasmid
    #140467
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for S-ketamine, termed iSKetSnFR2, thus enabling optical subcellular pharmacokinetics for S-Ketamine.
    Depositor
    Henry Lester
    Article
    Bera et al Front Cell Neurosci. 2019 Nov 12;13:499.
    Insert
    iSKetSnFR2
    Use
    Tags
    His Tag, HA Tag and Myc tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    140467-plasmid-map-sequence-id-296110

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  22. iSKetSnFR1

    Plasmid
    #140466
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for S-ketamine, termed iSKetSnFR1, thus enabling optical subcellular pharmacokinetics for S-Ketamine.
    Depositor
    Henry Lester
    Article
    Bera et al Front Cell Neurosci. 2019 Nov 12;13:499.
    Insert
    iSKetSnFR1
    Use
    Tags
    His Tag, HA Tag and Myc tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    140466-plasmid-map-sequence-id-296106

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  31. pET24a-VHH-1xTS

    Plasmid
    #109418
    Purpose
    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to one tyrosine sulfation (TS) motif. VHH-1xTS also contains a T7, HA, BAP and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115
    Insert
    anti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
    Use
    Tags
    BAP, HA, His6, T7, and Tyrosine sulfation (TS) se…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    109418-plasmid-map-sequence-id-211591

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  37. MSCV H-Ras V12 IRES GFP

    Plasmid
    #18780
    Depositor
    Scott Lowe
    Insert
    H-Ras V12 (HRAS Human)
    Use
    Retroviral
    Tags
    IRES GFP
    Expression
    Mammalian
    Mutation
    V12
    Promoter
    Available since
    July 3, 2008
    Availability
    Academic Institutions and Nonprofits only
    18780-plasmid-map-sequence-id-159508

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  39. mEmerald-Farnesyl-5

    Plasmid
    #54093
    Purpose
    Localization: Membrane, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 4401 - 4440 of 10096 results