Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 22,938 results for: Tes

Showing: 5501 - 5550 of 22938 results

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pVF14

Plasmid

#229455

Purpose

Expresses IraD with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pVF17

Plasmid

#229454

Purpose

Expresses IraM with His-tag

Depositor

Alexandra Deaconescu

Article

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraM

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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aTY173_pPGK1_mTagBFP2_WPRE

Plasmid

#225352

Purpose

Lentiviral vector for BFP expression

Depositor

Wendell Lim

Article

Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

Insert

BFP

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB602-MpPHOT-3xFLAG

Plasmid

#228474

Purpose

MpPHOT-3xFLAG under the control of the cauliflower mosaic virus (CaMV) 35S promoter (for Agrobacterium-mediated genetic transformation.)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

MpPHOT-3xFLAG

Expression

Bacterial and Plant

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-OXER1-mKate2

Plasmid

#226713

Purpose

overexpression red fluorescent OXER1 protein in zebrafish larvae

Depositor

Philipp Niethammer

Article

Ma et al Cell Rep. 2023 Jan 31;42(1):111974. doi:

Insert

OXER1 (OXER1 Human)

Use

Unspecified; Zebrafish expression

Tags

mKate2

Promoter

SP6

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pGGC018

Plasmid

#228232

Purpose

Level 0 for LhG4 (GreenGate C module)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

GR-LhG4

Use

Synthetic Biology

Promoter

-

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_A1-LCD swap V1

Plasmid

#226538

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD V1

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap V1

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap V2

Plasmid

#226539

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD V2

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap V2

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap V3

Plasmid

#226540

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD V3

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap V3

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap X5

Plasmid

#226547

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X5

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X5

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap X7

Plasmid

#226549

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X7

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X7

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap X1

Plasmid

#226543

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X1

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X1

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap V5

Plasmid

#226542

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD V5

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap V5

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap X4

Plasmid

#226546

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X4

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X4

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swapX3

Plasmid

#226545

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X3

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X3

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap V4

Plasmid

#226541

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD V4

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap V4

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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GFP H2B SL2

Plasmid

#227718

Purpose

Plasmid contains GFP H2B SL2, with some nlp-51 homology sequence after, Amp Resistant

Depositor

Oliver Hobert, Haosheng Sun

Article

Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

Insert

GFP H2B SL2 (nlp-51 Nematode)

Use

Cloning vector

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUCmini-RELA-PGK-EGFP

Plasmid

#228051

Purpose

Homologous donor template for RELA knockout expressing EGFP

Depositor

Yohei Sato

Article

Sato et al Clinical Immunology Communications, Volu

Insert

EGFP

Use

CRISPR

Expression

Mammalian

Promoter

PGK

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUCmini-RELB-PGK-EGFP

Plasmid

#228052

Purpose

Homologous donor template for RELB knockout expressing EGFP

Depositor

Yohei Sato

Article

Sato et al Clinical Immunology Communications, Volu

Insert

EGFP

Use

CRISPR

Expression

Mammalian

Promoter

PGK

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUCmini-RELC-PGK-EGFP

Plasmid

#228053

Purpose

Homologous donor template for RELC knockout expressing EGFP

Depositor

Yohei Sato

Article

Sato et al Clinical Immunology Communications, Volu

Insert

EGFP

Use

CRISPR

Expression

Mammalian

Promoter

PGK

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUCmini-RELA-PGK-tdTomato

Plasmid

#228054

Purpose

Homologous donor template for RELA knockout expressing tdTomato

Depositor

Yohei Sato

Article

Sato et al Clinical Immunology Communications, Volu

Insert

tdTomato

Use

CRISPR

Expression

Mammalian

Promoter

PGK

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUCmini-RELC-PGK-tdTomato

Plasmid

#228056

Purpose

Homologous donor template for RELC knockout expressing tdTomato

Depositor

Yohei Sato

Article

Sato et al Clinical Immunology Communications, Volu

Insert

tdTomato

Use

CRISPR

Expression

Mammalian

Promoter

PGK

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap X10

Plasmid

#226552

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X10

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X10

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap X9

Plasmid

#226551

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X9

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X9

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap X8

Plasmid

#226550

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X8

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X8

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap X6

Plasmid

#226548

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X6

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X6

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap X2

Plasmid

#226544

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X2

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X2

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS316-SEC18-L1374

Plasmid

#226275

Purpose

Plasmid expressing the SEC18 allele from L-1374, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC18 (SEC18 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS316-SEC18-S288C

Plasmid

#226274

Purpose

Plasmid expressing the SEC18 allele from S288C, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC18 (SEC18 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS316-SEC18-NCYC110

Plasmid

#226277

Purpose

Plasmid expressing the SEC18 allele from NCYC110, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC18 (SEC18 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS316-SEC18-UWOPS872421

Plasmid

#226276

Purpose

Plasmid expressing the SEC18 allele from UWOPS87-2421, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC18 (SEC18 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS313-SCT1-L1374

Plasmid

#226267

Purpose

Plasmid expressing the SCT1 allele from L-1374, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SCT1 (SCT1 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pML107-LUG1

Plasmid

#226265

Purpose

Plasmid expressing Cas9 and gRNA TCTTCAAGTTACTCCAAGAG which targets the LUG1 gene

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

LUG1 gRNA (YLR352W Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pML104-LEU2-2

Plasmid

#226263

Purpose

Plasmid expressing Cas9 and gRNA GGTAGTGTTAGACCTGAACA which targets the LEU2 gene

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

LEU2 gRNA (LEU2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS313-SCT1-NCYC110

Plasmid

#226269

Purpose

Plasmid expressing the SCT1 allele from NCYC110, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SCT1 (SCT1 Budding Yeast)

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS313-SCT1-UWOPS872421

Plasmid

#226268

Purpose

Plasmid expressing the SCT1 allele from UWOPS87-2421, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SCT1 (SCT1 Budding Yeast)

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Dm_λHA-FLuc100_CSC

Plasmid

#210592

Purpose

Expresses lambdaHA-optimal Firefly (CSC metric; Drosophila)

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

LambdaHA-optimal Firefly luciferase (Dm optimization, CSC metric)

Tags

lambda HA

Expression

Insect

Promoter

Ac5

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Dm_Opt_Renilla_CSC

Plasmid

#210593

Purpose

Expresses Optimal Renilla luciferase (CSC metric; Drosophila)

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Optimal Renilla luciferase (Optimized for Drosophila, CSC metric)

Expression

Insect

Promoter

Ac5

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUB_OptλHAramp-FLuc100

Plasmid

#210577

Purpose

Expresses Optimal lambda HA-Optimal Firefly luciferase

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Optimal lambda HA-Optimal Firefly luciferase

Tags

lambda HA

Expression

Mammalian

Promoter

UBC human

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUB_NonoptλHAramp-FLuc100

Plasmid

#210579

Purpose

Expresses Nonoptimal lambda HA-Optimal Firefly luciferase

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Nonoptimal lambda HA-Optimal Firefly luciferase

Use

Lentiviral and Luciferase

Tags

lambda HA

Expression

Mammalian

Promoter

UBC human

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_SM-FLuc100-pA

Plasmid

#210584

Purpose

Expresses SM(FLAG)-optimal Firefly luciferase - pA

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-optimal FLuc

Use

Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Dm_λHA-FLuc0_CSC

Plasmid

#210589

Purpose

Expresses lambdaHA-nonoptimal Firefly (CSC metric; Drosophila)

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

LambdaHA-nonoptimal Firefly luciferase (Dm optimization, CSC metric)

Use

Luciferase

Tags

lambda HA

Expression

Insect

Promoter

Ac5

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS3-tdc-1P::AI::acc-1::SL2::his-24::tagRFP::unc-54-3'UTR

Plasmid

#225926

Purpose

Expressing ACC-1 in RIM neuron

Depositor

Andrew Leifer

Article

Kumar et al bioRxiv [Preprint]. 2024 Mar 27:2024.03.

Insert

ACC-1 (acc-1 Nematode)

Expression

Worm

Promoter

tdc-1

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF-1α-p37-mClover2

Plasmid

#225500

Purpose

Expression of mClover2-tagged p37 protein

Depositor

David Rubinsztein

Article

Wrobel et al Sci Adv. 2024 May 3;10(18):eadl6082. doi

Insert

p37 (UBXN2B Human)

Use

Lentiviral

Tags

mClover2

Expression

Mammalian

Mutation

no

Promoter

EF1A

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5a-p37WT-FLAG

Plasmid

#225498

Purpose

Expression of FLAG-tagged wildtype p37 protein

Depositor

David Rubinsztein

Article

Wrobel et al Sci Adv. 2024 May 3;10(18):eadl6082. doi

Insert

p37 (UBXN2B Human)

Tags

FLAG

Expression

Mammalian

Mutation

no

Promoter

CMV promoter

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5a-p37SHPmut_FLAG

Plasmid

#225499

Purpose

Expression of FLAG-tagged mutated p37 protein

Depositor

David Rubinsztein

Article

Wrobel et al Sci Adv. 2024 May 3;10(18):eadl6082. doi

Insert

p37 (UBXN2B Human)

Tags

FLAG

Expression

Mammalian

Mutation

F215A/E218A/QKL220-222AAA

Promoter

CMV promoter

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS3-tdc-1P::AI::lgc-47::SL2::his-24::tagRFP::unc-54-3'UTR

Plasmid

#225925

Purpose

Expressing LGC-47 in RIM neuron

Depositor

Andrew Leifer

Article

Kumar et al bioRxiv [Preprint]. 2024 Mar 27:2024.03.

Insert

LGC-47 (lgc-47 Nematode)

Expression

Worm

Promoter

tdc-1

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS3-npr-9P::AI::lgc-47::SL2::tagBFP::unc-54-3'UTR

Plasmid

#225923

Purpose

Expressing LGC-47 in AIB neuron

Depositor

Andrew Leifer

Article

Kumar et al bioRxiv [Preprint]. 2024 Mar 27:2024.03.

Insert

LGC-47 (lgc-47 Nematode)

Expression

Worm

Promoter

npr-9

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSliceRep_B_miR-196a.M3

Plasmid

#220471

Purpose

Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B)

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

SUMO_Eu1

Tags

3xHA

Expression

Mammalian

Promoter

Minimal CMV

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUdOm8.1

Plasmid

#210288

Purpose

Empty backbone with zeocin resistance for high expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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