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Plasmid#178688PurposeBacterial expression of N-terminally 6His tagged A1-LCD with twenty Gly residues replaced with Ser, seven Tyr residues replaced with Phe (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD-20G+20S+7F-7Y (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationtwenty Gly residues replaced with Ser, seven Tyr …PromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD-14N-4Q+18G
Plasmid#178694PurposeBacterial expression of N-terminally 6His tagged A1-LCD with fourteen Gln residues removed, four Asn removed, replaced with eighteen Gly (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD-14N-4Q+18G (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationfourteen Gln residues removed, four Asn removed, …PromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD-20G+20S-12F+12Y
Plasmid#178689PurposeBacterial expression of N-terminally 6His tagged A1-LCD with twenty Gly residues replaced with Ser, twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD-20G+20S-12F+12Y (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationtwenty Gly residues replaced with Ser, twelve Phe…PromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD+23G-23S-12F+12Y
Plasmid#178691PurposeBacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Ser residues replaced with Gly, twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD+23G-23S-12F+12Y (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationtwenty-three Ser residues replaced with Gly, twel…PromoterAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD+23G-23S+7F-7Y
Plasmid#178690PurposeBacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Ser residues replaced with Gly, seven Tyr replaced with Phe (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD+23G-23S+7F-7Y (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationtwenty-three Ser residues replaced with Gly, seve…PromoterAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/mArf1(T31N)-HA
Plasmid#79411PurposeExpresses C-terminally HA-tagged Arf1(T31N) in mammalian cellsDepositorInsertArf1 (Arf1 Mouse)
UseTagsHAExpressionMammalianMutationchanged Threonine 31 to AsparaginePromoterAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/mArf3(T31N)-HA
Plasmid#79416PurposeExpresses C-terminally HA-tagged Arf3(T31N) in mammalian cellsDepositorInsertArf3 (Arf3 Mouse)
UseTagsHAExpressionMammalianMutationchanged Threonine 31 to AsparaginePromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-pfkB
Plasmid#102284PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting pfkB.DepositorInsertpfkB gRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpTetAvailable sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSG069
Plasmid#214260PurposepJN105 with PaftsH2SD and PaFtsH2 ORF cloned between NheI/XbaIDepositorInserttLST accessory genetic element encoded PaFtsH2 proteinase from Pseudomonas aeruginosa SG17M
UseTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
D73_Ld_262620
Plasmid#135886PurposeExpression of Leishmania donovani ectodomain in mammalian cellsDepositorInsertParaquat-inducible protein A, putative
UseTagsbiotinylation sequence and His tagExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_ABCB9_WT
Plasmid#82899PurposeGateway Donor vector containing ABCB9, part of the Target Accelerator Plasmid Collection.DepositorInsertABCB9 (ABCB9 Human)
UseGateway entry vectorTagsExpressionMutation582_590delISLVSQEPVinsVCARAWATL; 592_595delFARSin…PromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_ABCB9_p.R281L
Plasmid#82840PurposeGateway Donor vector containing ABCB9, part of the Target Accelerator Plasmid Collection.DepositorInsertABCB9 (ABCB9 Human)
UseGateway entry vectorTagsExpressionMutationR281RL; 582_590delISLVSQEPVinsVCARAWATL; 592_595d…PromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMY-MCS-P2A-eGFP
Plasmid#163356PurposeRetroviral backbone for in-frame cloning of a gene of interest and a P2A separated GFP markerDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMY-MCS-P2A-CD90.2
Plasmid#163355PurposeRetroviral backbone for in-frame cloning of a gene of interest and a P2A separated CD90.2 surface markerDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMY-MCS-P2A-Ly6G
Plasmid#163353PurposeRetroviral backbone for in-frame cloning of a gene of interest and a P2A separated Ly6G surface markerDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMY-MCS-P2A-BlastR-DYK
Plasmid#163357PurposeRetroviral backbone for in-frame cloning of a gene of interest and a P2A separated Blasticidin selection markerDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMY-MCS-P2A-PuroR-HA
Plasmid#163352PurposeRetroviral backbone for in-frame cloning of a gene of interest and a P2A separated Puromycin selection markerDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMY-MCS-P2A-CD90.1
Plasmid#163354PurposeRetroviral backbone for in-frame cloning of a gene of interest and a P2A separated CD90.1 surface markerDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTKA002
Plasmid#164578PurposeExpresses SARS-CoV-2 nsp8 from a pET46 backbone for IPTG-inducible bacterial expressionDepositorInsertnsp8
UseTags6xHis, Ek, TEVExpressionBacterialMutationPromoterT7 PromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGGF_RT_FAST_Venus
Plasmid#136980PurposeGoldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescenceDepositorInsertseed coat marker, replaced RFP w/ Venus. Replaced NOS terminator w/ Arabidopsis HSP18-2 terminator
UseTagsExpressionBacterialMutationBsaI site domestication in pOLE1:OLE1-RFP 688 G &…PromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only