We narrowed to 3,796 results for: golden gate

1. pEPOR1CB0114

   Plasmid

   #117553

   Purpose

   Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   Promoter_AtU6-26 + sgRNA_NbPDS1 N. benthamiana
   

   Expression

   Plant

   Promoter

   At U6-26 (pICSL90002)

   Available Since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEPOR1CB0029

   Plasmid

   #117541

   Purpose

   Level1 Golden Gate Cassette: sgRNA cassette for LbCas12a, targeting NbPDS gene in N. benthamiana

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   crRNA (LbCas12a)
   

   Expression

   Plant

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEPOR1CB0028

   Plasmid

   #117540

   Purpose

   Level1 Golden Gate Cassette: sgRNA cassette for AsCas12a, targeting NbPDS gene in N. benthamiana

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   crRNA (AsCas12a)
   

   Expression

   Plant

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEPOR1CB0022

   Plasmid

   #117537

   Purpose

   Level1 Golden Gate Cassette: estended stem sgRNA cassette for SpCas9 targeting NbPDS gene in N. benthamiana

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   sgRNA (Sp Cas9) - extended stem
   

   Expression

   Plant

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEPOR0SP0012

   Plasmid

   #117533

   Purpose

   Level0 Golden Gate part: CDS, eSaCas9 (no stop codon)

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   eSaCas9 (no stop codon)
   

   Use

   CRISPR

   Mutation

   R499A,Q500K,R654A,G655R

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEPOR0SP0018

   Plasmid

   #117528

   Purpose

   Level0 Golden Gate part: CDS, Sp-eCas9 1.1

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   Sp-eCas9 1.1 (with stop codon)
   

   Use

   CRISPR

   Mutation

   K848A, K1003A, R1060A

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEPOR0SP0015

   Plasmid

   #117525

   Purpose

   Level0 Golden Gate part: CDS, SpCas9-KA (no stop codon)

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   SpCas9-KA (no stop codon)
   

   Use

   CRISPR

   Mutation

   K855A

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEPOR0SP0014

   Plasmid

   #117524

   Purpose

   Level0 Golden Gate part: CDS, SpCas9-KA

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   SpCas9-KA (with stop codon)
   

   Use

   CRISPR

   Mutation

   K855A

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEPOR0SP0001

   Plasmid

   #117523

   Purpose

   Level0 Golden Gate part: CDS, SpCas9-DE (no stop codon)

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   SpCas9-DE (no stop codon)
   

   Use

   CRISPR

   Mutation

   D1135E

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEPOR0SP0009

    Plasmid

    #117522

    Purpose

    Level0 Golden Gate part: CDS, SpCas9-p (no stop codon)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SpCas9-p (no stop codon)
    

    Use

    CRISPR

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pICSL90005

    Plasmid

    #117520

    Purpose

    Level0 Golden Gate part: CDS, SpCas9-h (no stop codon)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SpCas9-h (no stop codon)
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEPOR0SP0008

    Plasmid

    #117535

    Purpose

    Level0 Golden Gate part: CDS, AsCas12a

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    AsCas12a (with stop codon)
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGGF004

    Plasmid

    #48845

    Purpose

    Provides a D-alanine resistance cassette as GreenGate module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    p35S::D-AlanineR::t35S
    

    Use

    Golden gate compatible cloning vector

    Mutation

    internal BsaI recognition site in 35S promoter re…

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ131A

    Plasmid

    #69273

    Purpose

    Golden Gate entry vector; 1st gRNA under AtU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSEED

    Plasmid

    #219658

    Purpose

    Permits GoldenGate assembly of new SEED cassettes via Golden Gate assembly (BsaI)

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    Empty SEED Cassette (3xP3-dsRed)
    

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pICH-roGFP2-ORP1

    Plasmid

    #191677

    Purpose

    Ti plasmid pICH86988 harbouring roGFP2-ORP1 for peroxide detection in plant cells.

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    redox(ro)GFP2-ORP1
    

    Use

    Binary agrobacterium ti plasmid

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDV044

    Plasmid

    #191824

    Purpose

    Plasmid for the transformation of the cyanobacterium Arthrospira platensis at the KmR locus. pCPC600 promoter. With terminator. Golden Gate compatible. Selection: in A. platensis: KmR; E. coli: AmpR

    Depositor

    Jim Roberts

    Article

    Tabakh et al STAR Protoc. 2023 Feb 8;4(1):102087. doi

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIVT-eeBxb1

    Plasmid

    #232748

    Purpose

    IVT template for making modRNA encoding eeBxb1

    Depositor

    Kate Galloway

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    Bxb1
    

    Use

    In vitro transcription

    Tags

    HA and SV40 NLS

    Mutation

    V74A, E229K, V375I

    Available Since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. scFv-HaloTag-iLID

    Plasmid

    #227390

    Purpose

    Adhesion module, scFv binds SunTag

    Depositor

    Clifford Brangwynne

    Article

    Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

    Insert

    scFv-HaloTag-iLID
    

    Use

    Lentiviral

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pXTK024 [Origin_RSF1010_PartType1]

    Plasmid

    #229261

    Purpose

    XanthoMoClo Golden Gate Part Plasmid for cloning, contains Origin_RSF1010_PartType1

    Depositor

    Michael Springer

    Article

    Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

    Insert

    RSF1010 Origin
    

    Expression

    Bacterial

    Available Since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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