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CIT1-2EGFP-ter-URA3

Plasmid

#184767

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at CIT1 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

CIT1 (CIT1 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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AFG3-2EGFP-ter-URA3

Plasmid

#184770

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at AFG3 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

AFG3 (AFG3 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iEscSnFR_PM

Plasmid

#182813

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_PM

Expression

Mammalian

Promoter

hCMV

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iEscSnFR_cyto

Plasmid

#182814

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_cyto

Expression

Mammalian

Promoter

hCMV

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iFluoxSnFR_ER

Plasmid

#182815

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iFluoxSnFR_ER

Expression

Mammalian

Promoter

hCMV

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iFluoxSnFR_PM

Plasmid

#182816

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iFluoxSnFR_PM

Expression

Mammalian

Promoter

hCMV

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iEscSnFR_ER

Plasmid

#182812

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_ER

Expression

Mammalian

Promoter

hCMV

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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SEPLuc-E2A-cyOFP1

Plasmid

#141084

Purpose

precursor of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosis

Depositor

Jeak Ling Ding

Publication

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

SEPLuc-E2A-cyOFP1

Use

Lentiviral

Promoter

CMV

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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P313-FBP_8

Plasmid

#139699

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing H3H

Depositor

Daniel Voytas

Publication

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:AtuOcs, pCmYLCV:NnHisps:tHsp, pAtuMas:Nn Luz:trbcSE9

Use

Luciferase

Expression

Plant

Promoter

various

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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ReNL-Actin_pcDNA3

Plasmid

#89531

Purpose

Red color variant of bright luminescent protein, fused to actin

Depositor

Takeharu Nagai

Publication

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

ReNL

Tags

Actin

Expression

Mammalian

Promoter

CMV

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pNB649

Plasmid

#60136

Purpose

Firefly luciferase (Promega) driven by MET17 promoter.

Depositor

Nicolas Buchler

Publication

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc

Expression

Yeast

Mutation

A4V

Promoter

MET17pr

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR-v2_sg2_Ero1a

Plasmid

#257614

Purpose

CRISPR knockout of Ero1a using sgRNA 2

Depositor

Kivanc Birsoy

Publication

Liu et al Nat Cell Biol. 2026 Apr 17. doi: 10.1038

Insert

sg2_Ero1a (ERO1A Mouse)

Use

Lentiviral

Promoter

U6

Available since

July 8, 2026

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR-v2_sg1_Ero1a

Plasmid

#257613

Purpose

CRISPR knockout of Ero1a using sgRNA 1

Depositor

Kivanc Birsoy

Publication

Liu et al Nat Cell Biol. 2026 Apr 17. doi: 10.1038

Insert

sg1_Ero1a (ERO1A Mouse)

Use

Lentiviral

Promoter

U6

Available since

July 8, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3-lyso-diGlo

Plasmid

#251837

Purpose

Outer lysosomal membrane-targeted control glutamine biosensor

Depositor

Danielle Schmitt

Publication

Scully et al RSC Chem Biol. 2026 Jul 13. doi: 10.1039

Insert

lyso-diGlo

Tags

LAMP1

Expression

Mammalian

Promoter

CMV

Available since

May 26, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3-nuc-diGlo

Plasmid

#251843

Purpose

Nuclear-localized control glutamine biosensor

Depositor

Danielle Schmitt

Publication

Scully et al RSC Chem Biol. 2026 Jul 13. doi: 10.1039

Insert

nuc-diGlo

Tags

2x NLS

Expression

Mammalian

Promoter

CMV

Available since

April 29, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3-matrix-diGlo

Plasmid

#251833

Purpose

Control mitochondrial matrix targeted glutamine biosensor

Depositor

Danielle Schmitt

Publication

Scully et al RSC Chem Biol. 2026 Jul 13. doi: 10.1039

Insert

matrix-diGlo

Tags

COX8

Expression

Mammalian

Promoter

CMV

Available since

April 29, 2026

Availability

Academic Institutions and Nonprofits only

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T7-LacO-Halo-PILATeS-RGD (200nM)

Plasmid

#216716

Purpose

Expresses HaloTag-PILATeS-RGD with 200 nM BiT tag in bacteria

Depositor

Alex Dunn

Publication

Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

Insert

HaloTag-PILATeS-RGD

Tags

6xHis and HaloTag

Expression

Bacterial

Available since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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T7-LacO-Halo-PILATeS-RGD (700pM)

Plasmid

#216714

Purpose

Expresses HaloTag-PILATeS-RGD with 700 pM BiT tag in bacteria

Depositor

Alex Dunn

Publication

Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

Insert

HaloTag-PILATeS-RGD

Tags

6xHis and HaloTag

Expression

Bacterial

Available since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pBP-P_TALEsp1_PUPsp1-lacI_Ptac-lacO

Plasmid

#204079

Purpose

Type 0 promoter part

Depositor

Mikhail Shapiro

Insert

TALEsp1-stabilized promoter driving lacI repressor; Ptac-lacO IPTG-inducible promoter

Expression

Bacterial

Mutation

None

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP9B(E272Q)-HA

Plasmid

#209245

Purpose

Mammalian expression of ATP9B mutant E272Q

Depositor

Hye-Won Shin

Publication

Tone et al FEBS Lett. 2020 Feb;594(3):412-423. doi:

Insert

ATP9B (ATP9B Human)

Tags

HA

Expression

Mammalian

Mutation

E272Q

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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