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pCAGEN-DV-IRES-myr-EGFP (JDW 495)

Plasmid

#242574

Purpose

Gateway destination vector with a CAGGS promoter in the backbone as well as an IRES-myr-EGFP to label cell membranes.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

attR1-CmR-ccdB-attR2

Expression

Mammalian

Promoter

CAGGS

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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VN1287 pB2Hws_v28_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP2_Ddcm_WeaKRBS_TIR51_KanR

Plasmid

#235116

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP2.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP2

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

LtetO and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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VN1262 pB2Hws_v28_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP3_Ddcm_WeaKRBS_TIR51_KanR

Plasmid

#235117

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP3.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP3

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

LtetO and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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VN550 pAC-cI_E34P-Asf1A_Term_f1-pOL2-62_L-eGFP-KanR

Plasmid

#235102

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1A.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Insert

Asf1A (ASF1A Human)

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P

Expression

Bacterial

Promoter

lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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VN991 pB2Hws_v9_cI-Asf1B+rpoA-IP1

Plasmid

#235105

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP1.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP1

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

lacUV5 and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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VN1043 pB2Hws_v9_cI-Asf1B+rpoA-IP2

Plasmid

#235106

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP2.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP2

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

lacUV5 and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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VN990 pB2Hws_v9_cI-Asf1B+rpoA-IP3

Plasmid

#235107

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP3.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP3

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

lacUV5 and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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VN1335 pB2Hws_v9_cI-Asf1B+rpoA-IP4

Plasmid

#235108

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP4.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP4

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

lacUV5 and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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VN1312 pB2Hws_v30_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP3mut3A_Ddcm_eGFP-wcKanR

Plasmid

#235109

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP3mut3A.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP3mut3A

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

LtetO and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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VN1313 pB2Hws_v30_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP1_Ddcm_eGFP-wcKanR

Plasmid

#235110

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP1.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP1

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

LtetO and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge