We narrowed to 5,869 results for: nls

1. pVE2_7_p40S_QfDBD_VP16AD_cp_phl_DsRed_5xQUAS

   Plasmid

   #154234

   Purpose

   Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_phl

   Depositor

   Arnold Driessen

   Article

   Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.

   Insert

   p40S:QfDBD-VP16AD-GFP-NLS
   

   Use

   Synthetic Biology; Fungal expression

   Tags

   GFP-NLS; DsRed-SKL

   Expression

   Bacterial

   Mutation

   Promoter

   p40S (AN0465 A. nidulans)

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLM2_45_pgndA_QfDBD_VP16AD_cp_pcbC_DsRed_0xQUAS

   Plasmid

   #154225

   Purpose

   Encodes pgndA:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter

   Depositor

   Arnold Driessen

   Article

   Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.

   Insert

   pgndA:QfDBD-VP16AD-GFP-NLS
   

   Use

   Synthetic Biology; Fungal expression

   Tags

   GFP-NLS; DsRed-SKL

   Expression

   Bacterial

   Mutation

   Promoter

   pGndA (An11g02040 A. niger)

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNJP

   Plasmid

   #115494

   Purpose

   Expression vector for NLS-iRFP-NLS, JNK KTR-mCherry, and mKO-MK2 in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Miura et al Cell Rep. 2018 Sep 4;24(10):2658-2668. d

   Insert

   NLS-iRFP-NLS-P2A-JNK KTR-mCherry-P2A-mKO-MK2 (MAPK14, MAPK8 Mouse, Human, Synthetic)
   

   Use

   Tags

   iRFP, mCherry, and mKO

   Expression

   Mammalian

   Mutation

   mCherry S227F, mCherry G229R, mKO V211G

   Promoter

   CAG

   Available since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-ccdB-boxB-tBE-V5-mA3

   Plasmid

   #171693

   Purpose

   Expresses tBE-V5-mA3 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

   Insert

   ccdB-sgRNA scaffold-boxB-tBE-V5-mA3
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and EF1a

   Available since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGRG36

   Plasmid

   #16666

   Depositor

   Nancy Craig

   Article

   McKenzie et al BMC Microbiol. 2006 . 6():39.

   Insert

   mTn7::MCS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGRG25

   Plasmid

   #16665

   Depositor

   Nancy Craig

   Article

   McKenzie et al BMC Microbiol. 2006 . 6():39.

   Insert

   mTn7::MCS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGRG37

   Plasmid

   #16667

   Depositor

   Nancy Craig

   Article

   McKenzie et al BMC Microbiol. 2006 . 6():39.

   Insert

   mTn7::Gateway cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. SOX17-tdTomato-EhB

   Plasmid

   #210467

   Purpose

   Donor plasmid for knock-in tdTomato into the human SOX17 locus

   Depositor

   Kai Wang

   Insert

   SOX17 homologous recombination arms with a NLS-tdTomato and EF1alpha-drived hygromycin-NLS-mTagBFP2 selection cassette (SOX17 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. lenti-Cas9-Exo-Blast-BFP

   Plasmid

   #196721

   Purpose

   Sortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions.

   Depositor

   Bernhard Schmierer

   Insert

   Cas9-Exo1-T2A-BSD-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKSE401

    Plasmid

    #62202

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    gRNA scaffold
    zCas9
    

    Use

    Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    maize codon optimized

    Promoter

    35S and AtU6-26p

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. BPK764

    Plasmid

    #65767

    Purpose

    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7 (x2)

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWMD23

    Plasmid

    #140006

    Purpose

    Expression of ORF1 (Shuffle 1 NLS) and Pong TPase (L418A, L420A)

    Depositor

    Charles Nathan Hancock

    Article

    Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

    Inserts

    ORF1 Shuffle 1 NLS
    Pong TPase LA
    

    Use

    Tags

    Expression

    Plant

    Mutation

    NES sequence mutated L418A, L420A and Ping N-term…

    Promoter

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. MSP469

    Plasmid

    #65771

    Purpose

    Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135V, R1335Q, and T1337R mutations in Cas9

    Promoter

    CMV & T7

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSP680

    Plasmid

    #65772

    Purpose

    Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135E, R1335Q, and T1337R mutations in Cas9

    Promoter

    CMV & T7

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSP2133

    Plasmid

    #72249

    Purpose

    Human expression plasmid for SpCas9-HF4 variant: CMV-T7-humanSpCas9-HF4(Y450A, N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 HF4(Y450A/N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    Y450A, N497A, R661A, Q695A, and Q926A

    Promoter

    CMV

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. MSP1673

    Plasmid

    #65769

    Purpose

    Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7 (x2)

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSP2135

    Plasmid

    #72248

    Purpose

    Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A, and D1135E

    Promoter

    CMV

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAGT6471

    Plasmid

    #219428

    Purpose

    Module of intronized NLS-SpCas9-NLS for N-terminal and C-terminal tag fusions (AGGT_N-SpCas9i-N_TTCG)

    Depositor

    Tom Schreiber

    Article

    Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

    Insert

    AGGT_NLS-SpCas9i-NLS(no stop)_TTCG
    

    Use

    Moclo compatible level 0 module

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-T7-AsCas12a-P2A-EGFP (RTW2861)

    Plasmid

    #160140

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AsCas12a with a c-terminal NLS(nucleoplasmin), 3x HA tag, and P2A-EGFP

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    human codon optimized AsCas12a with NLS(nucleoplasmin)-3xHA-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    NLS(nucleoplasmin)-3xHA-P2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV sMTase (eGFP)

    Plasmid

    #89930

    Purpose

    Expresses sMTase system for targeted DNA methylation in mammalian cells. Contains an eGFP marker expressed off separate promoter.

    Depositor

    Carl Novina

    Article

    Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

    Insert

    dCas9-MC-IRES-MN
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    deactivated Cas9

    Promoter

    CMV

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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