3,636 results

1. HA-dora[A]_rescue_construct

   Plasmid

   #190640

   Purpose

   Puromycin-selectable expression of HA-tagged truncated Dora (CG34401) in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   Dorado (CG34401 Fly)
   

   Use

   Tags

   3xHA

   Expression

   Insect

   Mutation

   Truncated to contain amino acids 1-945

   Promoter

   Actin-5c

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAc-sgRNA-Cas9.dora_1

   Plasmid

   #190700

   Purpose

   Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   dora (CG34401) sgRNA 1
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Drosophila U6

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAc-sgRNA-Cas9.dora_2

   Plasmid

   #190701

   Purpose

   Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   dora (CG34401) sgRNA 2
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Drosophila U6

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAc-sgRNA-Cas9.dora_3

   Plasmid

   #190702

   Purpose

   Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   dora (CG34401) sgRNA 3
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Drosophila U6

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX-6P-1 Drosophila melanogaster CBS-pair domain

   Plasmid

   #166436

   Purpose

   Express pGEX-6P-1 Drosophila melanogaster CBS-pair domain

   Depositor

   Kalle Gehring

   Article

   Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

   Insert

   Drosophila melanogaster CBS-pair domain (Cbs Fly)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac, LacI, Amp

   Available since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-15b(+) Drosophila melanogaster PRL (Δprenylation motif)

   Plasmid

   #166418

   Purpose

   Express pET-15b(+) Drosophila melanogaster PRL (Δprenylation motif)

   Depositor

   Kalle Gehring

   Article

   Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

   Insert

   Drosophila melanogaster PRL (Prl Fly)
   

   Use

   Tags

   N-terminal His-tag

   Expression

   Bacterial

   Mutation

   Deleted nucleotides coding for 4 C-terminal amino…

   Promoter

   AmpR, lac1

   Available since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pv6_dPC_2xChromo

   Plasmid

   #179398

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of dPC_2xChromo

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   dPC_2xChromo
   

   Use

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBABR mCherry-Bcd-LEXY

   Plasmid

   #182596

   Purpose

   Drosophila integration plasmid expressing mCherry-Bcd-LEXY fusion protein under the maternal tubulin promoter.

   Depositor

   Jared Toettcher

   Article

   Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

   Insert

   Bcd (bcd Fly)
   

   Use

   Tags

   LEXY and mCherry

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBABR Bcd-LEXY

   Plasmid

   #182594

   Purpose

   Drosophila integration plasmid expressing Bcd-LEXY fusion protein under the maternal tubulin promoter.

   Depositor

   Jared Toettcher

   Article

   Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

   Insert

   Bicoid (bcd Fly)
   

   Use

   Tags

   LEXY

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Donor_C_FH_VIR

    Plasmid

    #186660

    Purpose

    Donor plasmid to endogenously tag VIR gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    OSS VIR C-terminal 3xFlag-3xHA Tag Donor Plasmid (vir Fly)
    

    Use

    Tags

    3xFlag-3xHA

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GLE_sgRNA

    Plasmid

    #186658

    Purpose

    GLE sgRNA plasmid

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    Gle sgRNA Plasmid (CG14749 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Donor_N_FH_Shu

    Plasmid

    #186655

    Purpose

    Donor plasmid to endogenously tag Shu gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    OSS Shu N-terminal 3xFlag-3xHA Tag Donor Plasmid (shu Fly)
    

    Use

    Tags

    3xFlag-3xHA

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. piwi_sgRNA

    Plasmid

    #186653

    Purpose

    piwi sgRNA plasmid

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    Piwi sgRNA Plasmid (piwi Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Donor_N_FH_Piwi

    Plasmid

    #186651

    Purpose

    Donor plasmid to endogenously tag piwi gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    OSS PIWI N-terminal 3xFlag-3xHA Tag Donor Plasmid (piwi Fly)
    

    Use

    Tags

    3xFlag-3xHA

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAc5.1B-lambdaN-HA-DmPaip2-V5His6_Q

    Plasmid

    #147284

    Purpose

    Insect Expression of DmPaip2

    Depositor

    Elisa Izaurralde

    Insert

    DmPaip2
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAc5.1B-EGFP-DmPaip1_G

    Plasmid

    #146353

    Purpose

    Insect Expression of DmPaip1

    Depositor

    Elisa Izaurralde

    Insert

    DmPaip1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRSFDuetG-DmDCP1_128-372 (MCSI)_E

    Plasmid

    #146174

    Purpose

    Bacterial Expression of DmDcp1_128-372

    Depositor

    Elisa Izaurralde

    Insert

    DmDcp1_128-372 (Dcp1 Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    3 amino acid del (PQQ 318-320)

    Promoter

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAc5.1B-EGFP-DmDCP1-Y34A_P

    Plasmid

    #147214

    Purpose

    Insect Expression of DmDCP1-Y34A

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

    Insert

    DmDCP1-Y34A (Dcp1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAc5.1B-HA-DmDCP1-N41AS100A-dsRNAres_P

    Plasmid

    #147215

    Purpose

    Insect Expression of DmDCP1-N41AS100A-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

    Insert

    DmDCP1-N41AS100A-dsRNAres (Dcp1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAc5.1B-HA-DmDCP1-Y34F-dsRNAres_P

    Plasmid

    #147217

    Purpose

    Insect Expression of DmDCP1-Y34F-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

    Insert

    DmDCP1-Y34F-dsRNAres (Dcp1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge