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pWS060

Plasmid

#123024

Purpose

Pheromone responsive promoter

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pFUS1 [2]

Expression

Yeast

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pDM1019

Plasmid

#108976

Purpose

shuttle vector, N-terminal GFP tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum shuttle vector

Tags

GFP

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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tol2-mpx-lyn C466A-GFP

Plasmid

#35038

Depositor

Anna Huttenlocher

Article

Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

Insert

Lyn C466A-GFP (lyn Zebrafish)

Use

Zebrafish expression

Tags

GFP

Expression

Mammalian

Available Since

March 5, 2012

Availability

Academic Institutions and Nonprofits only
pARC (pKD-G3)

Plasmid

#62682

Purpose

Mammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

amiR-eGFP123

Use

RNAi

Expression

Mammalian

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pATI (pKD-G7)

Plasmid

#62686

Purpose

Mammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

amiR-eGFP123/amiR-eGFP419

Use

RNAi

Expression

Mammalian

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0011

Plasmid

#117530

Purpose

Level0 Golden Gate part: CDS, Sp-xCas9 3.7 (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Sp-xCas9 3.7 (no stop codon)

Use

CRISPR

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pDM1220

Plasmid

#108993

Purpose

integrating expression vector

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum integrating expression v…

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pUAST td tomato Rab35 CA

Plasmid

#58383

Purpose

expression of drosophila tdtomato-Rab35 constitutively active mutant

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Fly)

Tags

td tomato

Expression

Insect

Mutation

Q67L (constitutive active)

Promoter

GAL4

Available Since

July 21, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-EPN-01(ΔM)

Plasmid

#80047

Purpose

Expression or G2A mutants of EPN-01

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01

Tags

Myc

Expression

Mammalian

Mutation

M1I

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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hIRF-1 gRNA #409 (Sa)

Plasmid

#98134

Purpose

Expresses a guide RNA (gRNA) to target human IRF-1 promoter for the Sa-Cas9 system

Depositor

Hodaka Fujii

Article

Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

Insert

gRNA_hIRF1 promoter #409

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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hIRF-1 gRNA #351 (Sa)

Plasmid

#105283

Purpose

Expresses a guide RNA (gRNA) to target human IRF-1 promoter for the Sa-Cas9 system

Depositor

Hodaka Fujii

Article

Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

Insert

gRNA_hIRF1 promoter #351

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAWV

Plasmid

#62710

Purpose

Donor vector for amiR-eGFP knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

tdTomato/amiR-eGFP123/amiR-eGFP419/FLPe

Use

RNAi

Expression

Mammalian

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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VV220: CoChR(C108S)-eGFP in fck

Plasmid

#58512

Purpose

Expresses CoChR with the C108S (step function opsin) mutation fused to eGFP under the a-CamKII promoter

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

CoChR(C108S)-eGFP

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

changed Cysteine 108 to Serine

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-18 (1wa3)

Plasmid

#80123

Purpose

PH-4GS-I3-01-myc-Late1 (1wa3)

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-18 (1wa3)

Expression

Bacterial and Mammalian

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pCMV-EPN-25 (1wa3)

Plasmid

#80124

Purpose

I3-01p-myc-Late1-PH (1wa3)

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-25 (1wa3)

Expression

Bacterial and Mammalian

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01*(ΔL1+L2)

Plasmid

#80044

Purpose

Expression of EPN-01* mutated in both L domains

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01*(L33R)

Tags

Myc

Expression

Mammalian

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01-(ΔLF)

Plasmid

#80052

Purpose

Expression of Vpr binding mutant EPN-01

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01(deltaLF)

Tags

Myc

Expression

Mammalian

Mutation

LF45AA

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pET29b-EPN01(∆L1+∆L2)-His

Plasmid

#84511

Purpose

E.coli version of EPN01(∆L1+∆L2)

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN01(∆L1+∆L2)-His

Tags

His tag

Expression

Bacterial

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSuper 44-2

Plasmid

#51598

Purpose

shRNA 2 against rat Sema4D

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

shRNA2

Use

RNAi

Promoter

U6

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pUAST myc mouse Rab35 DN

Plasmid

#53542

Purpose

expression of myc tagged mouse Rab35 DN mutant

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Mouse)

Tags

myc

Expression

Insect

Mutation

S22N

Promoter

GAL4

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only