Addgene

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pUPD+35St

Plasmid

#170886

Purpose

Phytobrick - 35St

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

35St

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27Linear-2, PolyU(0)

Plasmid

#166994

Purpose

Tests for the impact of a synthetic linear tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(0)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-1, PolyU(0)

Plasmid

#166992

Purpose

Tests for the impact of a synthetic linear tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(0)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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GLY, Linear-3, PolyU(1)

Plasmid

#166988

Purpose

Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a GLNYtRNA promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(1)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-1_PolyU(1)

Plasmid

#159479

Purpose

Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-1_PolyU(1)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6-27

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-1_PolyU(2)

Plasmid

#159480

Purpose

Tests for the impact of 2 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-1_PolyU(2)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6-27

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-1_PolyU(3)

Plasmid

#159481

Purpose

Tests for the impact of 3 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-1_PolyU(3)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6-27

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pZF-BBB

Plasmid

#127470

Purpose

coding sequence for synthetic zinc finger ZF-BBB

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-BBB synthetic transcription factor

Use

Cell-free transcription–translation

Tags

Expression

Mutation

Promoter

T7

Available since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pZF-BCC

Plasmid

#127484

Purpose

coding sequence for synthetic zinc finger ZF-BCC

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-BCC synthetic transcription factor

Use

Cell-free transcription–translation

Tags

Expression

Mutation

Promoter

T7

Available since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pZF-ACA

Plasmid

#127466

Purpose

coding sequence for synthetic zinc finger ZF-ACA

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-ACA synthetic transcription factor

Use

Cell-free transcription–translation

Tags

Expression

Mutation

Promoter

T7

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pZF-BCD

Plasmid

#127472

Purpose

coding sequence for synthetic zinc finger ZF-BCD

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-BCD synthetic transcription factor

Use

Cell-free transcription–translation

Tags

Expression

Mutation

Promoter

T7

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pZF-BAB

Plasmid

#127469

Purpose

coding sequence for synthetic zinc finger ZF-BAB

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-BAB synthetic transcription factor

Use

Cell-free transcription–translation

Tags

Expression

Mutation

Promoter

T7

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pZF-BDC

Plasmid

#127473

Purpose

coding sequence for synthetic zinc finger ZF-BDC

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-BDC synthetic transcription factor

Use

Cell-free transcription–translation

Tags

Expression

Mutation

Promoter

T7

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pZF-BDD

Plasmid

#127474

Purpose

coding sequence for synthetic zinc finger ZF-BDD

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-BDD synthetic transcription factor

Use

Cell-free transcription–translation

Tags

Expression

Mutation

Promoter

T7

Available since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pZF-ADD

Plasmid

#127468

Purpose

coding sequence for synthetic zinc finger ZF-ADD

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-ADD synthetic transcription factor

Use

Cell-free transcription–translation

Tags

Expression

Mutation

Promoter

T7

Available since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pZF-ADB

Plasmid

#127467

Purpose

coding sequence for synthetic zinc finger ZF-ADB

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-ADB synthetic transcription factor

Use

Cell-free transcription–translation

Tags

Expression

Mutation

Promoter

T7

Available since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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SAH0141-0.0-conP01

Plasmid

#79828

Purpose

Transcriptional interference assessment; strong interfering promoter, landing site for spacers

Depositor

Katja Arndt

Article

Hoffmann et al Nucleic Acids Res. 2016 Jun 2;44(10):e95

Inserts

Blue fluorescent protein
mCherry

Use

Tags

Expression

Mutation

Promoter

bla P3 and conP01

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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SAH0146-0.5-conP01

Plasmid

#79831

Purpose

Transcriptional interference assessment; strong interfering promoter, 500 bp spacer

Depositor

Katja Arndt

Article

Hoffmann et al Nucleic Acids Res. 2016 Jun 2;44(10):e95

Inserts

Blue fluorescent protein
mCherry

Use

Tags

Expression

Mutation

Promoter

bla P3 and conP01

Available since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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SAH0098-scrPX

Plasmid

#79830

Purpose

Transcriptional interference assessment; no interfering promoter (scrambled)

Depositor

Katja Arndt

Article

Hoffmann et al Nucleic Acids Res. 2016 Jun 2;44(10):e95

Inserts

Blue fluorescent protein
mCherry

Use

Tags

Expression

Mutation

Promoter

bla P3 and scrPX (no promoter activity)

Available since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pKT-IDH1(R132H)-IRES-Katushka

Plasmid

#124257

Purpose

Expresses IDH1 with a R132H mutation and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

Insert

Isocitrate dehydrogenase 1 (R132H) (IDH1 Human)

Use

Tags

Expression

Mammalian

Mutation

Arginine 132 is mutated to Histidine. This mutati…

Promoter

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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