We narrowed to 24,446 results for: lars

1. pAPInt-mCherry-G418

   Plasmid

   #236490

   Purpose

   Plasmid for deleting or C-terminally tagging endogenous genes with mCherry and selection on G418.

   Depositor

   Daniel Lew

   Article

   Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Codon optimized for A. pullulans

   Promoter

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAPInt-sfGFP-G418

   Plasmid

   #236481

   Purpose

   Plasmid for deleting or C-terminally tagging endogenous genes with sfGFP and selection on G418.

   Depositor

   Daniel Lew

   Article

   Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Codon optimized for A. pullulans

   Promoter

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAPInt-Dendra2-G418

   Plasmid

   #236496

   Purpose

   Plasmid for deleting or C-terminally tagging endogenous genes with Dendra2 and selection on G418.

   Depositor

   Daniel Lew

   Article

   Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

   Insert

   Dendra2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Codon optimized for A. pullulans

   Promoter

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAPInt-mScarlet-G418

   Plasmid

   #236493

   Purpose

   Plasmid for deleting or C-terminally tagging endogenous genes with mScarlet and selection on G418.

   Depositor

   Daniel Lew

   Article

   Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

   Insert

   mScarlet
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Codon optimized for A. pullulans

   Promoter

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAPInt-GFP-G418

   Plasmid

   #236478

   Purpose

   Plasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on G418.

   Depositor

   Daniel Lew

   Article

   Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

   Insert

   GFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Codon optimized for A. pullulans

   Promoter

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDONOR 6colors

   Plasmid

   #229974

   Purpose

   bigMamAct assembled plasmid for 6colors cellular labelling

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Insert

   mito-mCherry, EYFP-tubulin, H2B iRFP720, GTS-mTagBFP, mTFP1-actin
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   See Depositor Comments

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBTK1061

   Plasmid

   #209550

   Purpose

   BTK assembled plasmid for integrating a KanR+E2-Crimson cassette into the S. alvi genome in place of the clpA gene

   Depositor

   Jeffrey Barrick

   Article

   Lariviere et al bioRxiv 2023.09.19.558440

   Insert

   E2-Crimson cassette flanked with homology arms for recombination into the S. alvi wkB2 genome to replace clpA (SALWKB2_RS04465)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Rp51a Hyperstabilized 5'SS

   Plasmid

   #100988

   Purpose

   pUC18 Hyperstabilized Rp51a 5'SS between HindIII and EcoRI; ATG/GTATGT -> AAG/GTAAGT

   Depositor

   Aaron Hoskins

   Article

   Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

   Insert

   RP51A (RPS17A Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   5'ss mut ATG/GTATGT -> AAG/GTAAGT

   Promoter

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. RP51A-NoBS-NoPseudoBS

   Plasmid

   #100986

   Purpose

   RP51A w/ T7 promoter in HindIII/EcoRI sites of pUC18. Has WT 5'ss but mutant BS and no pseudo BS

   Depositor

   Aaron Hoskins

   Article

   Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

   Insert

   RP51A (RPS17A Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   BS mutated to GTTAGTG, pseudo BS mutated to GTTTG…

   Promoter

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTALE-STAR-T_RFP entry

    Plasmid

    #87526

    Purpose

    Destination vector for domain insertion

    Depositor

    Steve Pollard

    Article

    Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

    Type

    Empty backbone

    Use

    Synthetic Biology and TALEN; Tale empty functiona…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTALE-STAR-A_RFP entry

    Plasmid

    #87527

    Purpose

    Destination vector for domain insertion

    Depositor

    Steve Pollard

    Article

    Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

    Type

    Empty backbone

    Use

    Synthetic Biology and TALEN; Tale empty functiona…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTALE-STAR-C_RFP entry

    Plasmid

    #87528

    Purpose

    Destination vector for domain insertion

    Depositor

    Steve Pollard

    Article

    Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

    Type

    Empty backbone

    Use

    Synthetic Biology and TALEN; Tale empty functiona…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTALE-STAR-G_RFP entry

    Plasmid

    #87529

    Purpose

    Destination vector for domain insertion

    Depositor

    Steve Pollard

    Article

    Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

    Type

    Empty backbone

    Use

    Synthetic Biology and TALEN; Tale empty functiona…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. NW50

    Plasmid

    #133445

    Purpose

    Covalently circularized nanodisc NW50 for studying membrane proteins and viral entry.

    Depositor

    Gerhard Wagner

    Article

    Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

    Insert

    NW50
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. NW30

    Plasmid

    #133444

    Purpose

    Covalently circularized nanodisc NW30 for studying membrane proteins and viral entry.

    Depositor

    Gerhard Wagner

    Article

    Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

    Insert

    NW30
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. circRNA-synIRES-R25-mNeonGreen

    Plasmid

    #188115

    Purpose

    Template plasmid to transcribe circular RNA encoding mNeonGreen

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET21a-Streptavidin-Alive

    Plasmid

    #20860

    Purpose

    Alive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.

    Depositor

    Alice Ting

    Article

    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

    Insert

    Streptavidin-Alive
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Wt gene

    Promoter

    Available since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET21a-Streptavidin-Dead

    Plasmid

    #20859

    Purpose

    Dead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).

    Depositor

    Alice Ting

    Article

    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

    Insert

    Streptavidin-Dead
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Triple mutant of wt Streptavidin N23A,S27D,S45A

    Promoter

    Available since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. circRNA-synIRES-R25-NanoLuc

    Plasmid

    #188116

    Purpose

    Template plasmid to transcribe circular RNA encoding NanoLuc

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    NanoLuc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NW9

    Plasmid

    #133442

    Purpose

    Covalently circularized nanodisc NW9 for studying membrane proteins and viral entry.

    Depositor

    Gerhard Wagner

    Article

    Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

    Insert

    NW9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge