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HA-MBP-TEVpCS-ADGRG7 CTF-FLAG

Plasmid

#217705

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGR7 (ADGRG7 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-415

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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LBR(R583Q)-FKBP-GFP

Plasmid

#222423

Purpose

To express a cholesterol synthesis point mutant of LBR (R583Q) with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

LBR(R583Q) (LBR Human)

Tags

FKBP-GFP

Expression

Mammalian

Mutation

R583Q

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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LBR(N547D)-FKBP-GFP

Plasmid

#222422

Purpose

To express a cholesterol synthesis point mutant of LBR (N547D) with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

LBR(N547D) (LBR Human)

Tags

FKBP-GFP

Expression

Mammalian

Mutation

N547D

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-ccdB-mRuby3

Plasmid

#166142

Purpose

Gateway destination vector for generation of Galactose-inductibe, C-terminal mRuby3-tagged proteins in yeast. Contains a 2um element for high copy number when in yeast.

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Type

Empty backbone

Tags

mRuby3

Expression

Yeast

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRG4 CTF-FLAG

Plasmid

#217702

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRG4 (ADGRG4 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-2721

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRF1 CTF-FLAG

Plasmid

#217694

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRF1 (ADGRF1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-566

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRD2 CTF-FLAG

Plasmid

#217688

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRD2 (ADGRD2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-636

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRD1 CTF-FLAG

Plasmid

#217687

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRD1 (ADGRD1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-544

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRF5 CTF-FLAG

Plasmid

#217698

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRF5 (ADGRF5 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-990

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRG1 CTF-FLAG

Plasmid

#217699

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRG1 (ADGRG1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-382

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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