We narrowed to 9,749 results for: Gnas
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Plasmid#123335PurposeCyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.DepositorInsertCerulean3-Cerulean3-FLARE-AKAR
UseTags6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
LIC-Z-delCry2
Plasmid#153545Purposethis is the non-clustering version of LIC-Z tagged with mcherryDepositorInserthuman TCR ζ-Chain (CD247 Human)
UseTagsmCherryExpressionMammalianMutationwith Cry2 sequence deleted from LIC-ZPromoterCMVAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-TYK2VF/PA-VSV
Plasmid#139351PurposeExpression of the double mutant of TYK2-V678F-P1104ADepositorInsertTYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
UseTagsVSVExpressionMammalianMutationV362F-V678F-P1104APromoterCMVAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
sgRNA BRCA2 R2896C
Plasmid#139511PurposePlasmid expressing a sgRNA to introduce BRCA2 R2896C using base editingDepositorInsertsgRNA to insert BRCA2 E2896C using base editing
UseTagsExpressionMammalianMutationPromoterU6Available sinceMay 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pBB0238-TEV-His12
Plasmid#137038Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0238 with C-terminal TEV-protease-cleavable His12DepositorInsertAAC66635.2
UseTagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length protein, contains signal p…PromoterT7Available sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
BtGRK2[D110A K220R]-mVenus
Plasmid#137779PurposeVisualization of GRK2DepositorInsertBovine GRK2[D110A K220R] (GRK2 Bovine)
UseTagsExpressionMammalianMutationD110A K220RPromoterCMVAvailable sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
HsGRK6[L66A E514A]-sYFP2
Plasmid#137781PurposeVisualization of GRK6DepositorInsertHuman GRK6[L66A E514A] (GRK6 Human)
UseTagsExpressionMammalianMutationL66A E514APromoterCMVAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-Flag-natT1R2 RR837-8GG
Plasmid#113958Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variantDepositorInsertT1R2 (TAS1R2 Human)
UseTagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationresidues 22-839, R837G and R838G substitutionPromotercmvAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
NES-PAmCherry-cPHx1
Plasmid#116859PurposePI(3,4)P2 biosensorDepositorInsertPLEKHA1 (PLEKHA1 Human)
UseTagsPAmCherryExpressionMammalianMutationamino acids 169-329PromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_cyt-AKAR5
Plasmid#114138PurposeMammalian expression of the AKAR5 sensor tagged with nucleus export signal (NES) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInsertAKAR5 with C-terminal nuclear export signal (NES)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-puro-eGFP-HEC1-M4
Plasmid#114036PurposeExpression of HEC1 M4 mutant tagged with GFPDepositorInsertGFP tagged - HEC1 -M4 (NDC80 Human)
UseTagsExpressionMammalianMutationM4 (K146E, R153E, K156E)PromoterCMV TetOAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-puro-eGFP-HEC1-M2
Plasmid#114035PurposeExpression of HEC1 M2 mutant tagged with GFPDepositorInsertGFP tagged - HEC1 -M2 (NDC80 Human)
UseTagsExpressionMammalianMutationM2 (K81E, N87E, K89E)PromoterCMV TetOAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
KG#84
Plasmid#110878PurposeExpresses the C. elegans acy--1 P260S gain-of-function cDNA in ventral cord cholinergic motor neuronsDepositorInsertsunc-17beta promoter
acy-1 P260S gain-of-function cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationChanged Proline to Serine at amino acid 260PromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLU-CMV-Flag-hSesn2 L373A/N376A/D385A/S387A
Plasmid#111789PurposeMammalian expression or 3rd generation lentivirus generation of hSesn2DepositorInserthSesn2 (SESN2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationL373A/N376A/D385A/S387APromoterAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
KG#81
Plasmid#110876PurposeExpresses the C. elegans acy-1 P260S gain-of-function cDNA in body wall muscleDepositorInsertsmyo-3 promoter
acy-1 P260S gain-of-function cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationChanged Proline to Serine at amino acid 260PromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
KG#83
Plasmid#110877PurposeExpresses the C. elegans acy--1 P260S gain-of-function cDNA pan-neuronallyDepositorInsertsrab-3 promoter
acy-1 P260S gain-of-function cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationChanged Proline to Serine at amino acid 260PromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSG424 ELKC(S383A)
Plasmid#89334PurposeElk1 cDNA encoding aa 307-428, aa383 mutated from ser-Ala, The PCR product was cut with BamH1 and Xba 1(365bp). pSG424 was cut with Bam H1 and Xba1. The Elk1 fragment was ligated into the BamH1DepositorInsertELK1 (ELK1 Human)
UseTagsGal 4ExpressionMammalianMutationencodes aa 307-428, changed serine 383 to alanine…PromoterAvailable sinceApril 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestGFP_RBPMS2
Plasmid#65747Purposeexpresses GFP-tagged RBPMS2 in mammalian cellsDepositorInsertRBPMS2 (RBPMS2 Human)
UseTagsGFPExpressionMammalianMutationS13N mutation in RBPMS2PromoterCMV, 2x TetAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
213-petDuet1-GBD-LUMP--cdc42
Plasmid#65973PurposeDual expression of GBD-LUMP and cdc42DepositorInsertsUseTagsGBD (GTPase binding domain from ACK1; residues 44…ExpressionBacterialMutationconstitutively active Cdc42 (Q61L)PromoterT7Available sinceJuly 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQCXIB-FLAG-hDIXDC1-L-WT
Plasmid#61224PurposeRetrovirus expressing long isoform of human DIXDC1DepositorInserthuman DIXDC1 (DIXDC1 Human)
UseRetroviralTagsFLAGExpressionMutationPromoterAvailable sinceMarch 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR-TER+-shDIXDC1-Hu-16
Plasmid#61237PurposeEntry clone encoding shRNA to human DIXDC1DepositorInsertDIXDC1 (DIXDC1 Human)
UseEntr cloneTagsExpressionMutationPromoterAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Blast-FLAG-hDIXDC1 WT
Plasmid#61220PurposeLentivirus expressing long isoform of human DIXDC1DepositorInserthuman DIXDC1 (DIXDC1 Human)
UseLentiviralTagsFLAGExpressionMutationPromoterPGKAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Hygro-FLAG-hDIXDC1 WT
Plasmid#61213PurposeRetrovirus expressing long isoform of human DIXDC1DepositorInserthuman DIXDC1 (DIXDC1 Human)
UseRetroviralTagsFLAGExpressionMutationPromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV/TO-Puro-FLAG-hDIXDC1 WT
Plasmid#61218PurposeLentivirus expressing long isoform of human DIXDC1DepositorInserthuman DIXDC1 (DIXDC1 Human)
UseLentiviralTagsFLAGExpressionMutationPromoterCMV/TOAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR-MYC-hDIXDC1-L-F630S
Plasmid#61241PurposeEntry clone encoding long isoform of human DIXDC1DepositorInserthuman DIXDC1 (DIXDC1 Human)
UseEntr cloneTagsMYCExpressionMutationChanged Phenylalanine 630 to SerinePromoterAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR-MYC-hDIXDC1-L-S592A
Plasmid#61240PurposeEntry clone encoding long isoform of human DIXDC1DepositorInserthuman DIXDC1 (DIXDC1 Human)
UseEntr cloneTagsMYCExpressionMutationChanged Serine 592 to AlaninePromoterAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR-MYC-hDIXDC1-L-WT
Plasmid#61239PurposeEntry clone encoding long isoform of human DIXDC1DepositorInserthuman DIXDC1 (DIXDC1 Human)
UseEntr cloneTagsMYCExpressionMutationPromoterAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR-FLAG-hDIXDC1-L-S592A
Plasmid#61211PurposeEntry clone encoding long isoform of human DIXDC1DepositorInserthuman DIXDC1 (DIXDC1 Human)
UseEntr cloneTagsFLAGExpressionMutationChanged Serine 592 to AlaninePromoterAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR-FLAG-hDIXDC1-L-F630S
Plasmid#61212PurposeEntry clone encoding long isoform of human DIXDC1DepositorInserthuman DIXDC1 (DIXDC1 Human)
UseEntr cloneTagsFLAGExpressionMutationChanged Phenylalanine 630 to SerinePromoterAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV-10-3xFLAG-LGP2-MI
Plasmid#58682PurposeExpresses LGP2 with a mutation to motif I in mammalian cellsDepositorInsertLGP2-MI (DHX58 Human)
UseTags3x FLAGExpressionMammalianMutationLysine 30 mutated to AlaninePromoterCMVAvailable sinceJuly 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
CS2+MYC-Etv5:T139D
Plasmid#49097Purposeexpression of Etv5 T139D mutantDepositorInsertEtv5 T139D (etv5a Zebrafish)
UseXenopus/avian/zebrafishTagsMYCExpressionMammalianMutationT139DPromoterCMVAvailable sinceNov. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
Cs2+Etv5 S142D
Plasmid#49096Purposeexpression of Etv5 S142D mutantDepositorInsertEtv5 S142 D (etv5a Zebrafish)
UseXenopus/avian/zebrafishTagsExpressionMammalianMutationS142DPromoterCMVAvailable sinceNov. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
TRS6E1b-luc(-732/-721) mutant 3
Plasmid#45386DepositorInsert6 copies of hPAI-1 promoter -732/-721 mutated at -723/-721
UseLuciferaseTagsluciferaseExpressionMammalianMutation-723/-721 mutated from tgt to acaPromoterAvailable sinceJune 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
TRS6E1b-luc(-732/-721) mutant 2
Plasmid#45385DepositorInsert6 copies of hPAI-1 promoter -732/-721 mutated at -730/-728
UseLuciferaseTagsluciferaseExpressionMammalianMutationmutated at -730/-728 from aca to tgtPromoterAvailable sinceJune 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
EGFP G protein Gamma 3(F70A,F71A) in pcDNA3.1
Plasmid#42185DepositorInsertG protein Gamma 3(F70A, F71A) (Gng3 Mouse)
UseTagsEGFPExpressionMammalianMutationF70A, F71APromotercmvAvailable sinceMarch 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
EGFP G protein Gamma 3(K68A,K69A) in pcDNA3.1
Plasmid#42188DepositorInsertGamma 3(K68A,K69A) (Gng3 Mouse)
UseTagsEGFPExpressionMammalianMutationK68A,K69APromotercmvAvailable sinceMarch 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
alpha 0-CFP in pdest12.2
Plasmid#36312DepositorInsertG protein alpha 0 (Gnao1 Rat)
UseTagsCFPExpressionMammalianMutationIntroduced KpnI and SpeI sites between 92nd and 9…PromoterCMVAvailable sinceFeb. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCin4-3xFLAG-MKL1 L555A
Plasmid#19851DepositorInsertMKL1 L555A (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationchanged Leucine 555 to AlaninePromoterAvailable sinceFeb. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
XE159 GFP-delta LZ-XDpr-delta PDZB-CS2+
Plasmid#16763DepositorInsertdelta LZ-XDpr-delta PDZB (dact1-b Frog)
UseXenopus expressionTagsgfpExpressionMutationXDpr that is doubly truncated to delete the first…PromoterAvailable sinceMarch 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFastBacHT (His10-4xFRB-SNAP-Lck G2A)
Plasmid#177887PurposeBaculo expression of decahistidine tag fused to 4xFRB, 1xSNAP tag and human Lck G2ADepositorInsertLck (LCK Human)
UseTags10xHis-TEV-4xFRB-SNAP tagExpressionInsectMutationG2APromoterPolyhedrinAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
HIP-flash (HOP-flash mutant)
Plasmid#83466PurposeNegative control for HOP-flash. 7xmut TEAD binding sites with minimal promoter plus luciferase reporter geneDepositorInsertMultimerized mutated TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
HA Depdc5 pRK5
Plasmid#46327DepositorInsertDepdc5 (DEPDC5 Human)
UseTagsHAExpressionMammalianMutationDiffers from Depdc5 isoform 1 by 10 amino acids …PromoterAvailable sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO/GFPZNF598-9P-9A
Plasmid#141192PurposeExpresses GFP-ZNF598 mutated in polyproline streches in mammalian cells, can be used to make inducible cell lineDepositorInsertZNF598 (ZNF598 Human)
UseTagsGFPExpressionMammalianMutationall 3 proline repeats are mutated to Alanine (9xP…PromoterCMVAvailable sinceJune 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL4.27_ARE/NRF2-SPE
Plasmid#177775PurposeLuciferase reporter for ARE/NRF2DepositorInsertNRF2 (NFE2L2 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT3-myr-AKT-HA
Plasmid#31789DepositorInsertAkt1 (Akt1 Mouse)
UseTagsHA and MyrExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
Lentiviral-TOP-dGFP-reporter
Plasmid#14715PurposeBeta-catenin reporter - 3rd generation self-inactivating lentiviral plasmid LEF-1/TCF-responsive promoter driven d2-eGFP (destabilized, half-life of 2h).DepositorInsertLEF-1/TCF-responsive promoter (Ctnnb1 )
UseLentiviralTagsd2 eGFPExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pPGK-T7/2-CD44s (fl)
Plasmid#137812Purposeexpression of CD44 proteins in mammalian cellsDepositorInsertCD44s (fl) (CD44 Human)
UseTagsGFPExpressionMammalianMutationfull length, I186T and I253V on CD44PromoterPGKAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-CAAX
Plasmid#86056PurposeA mammalian expression plasmid encoding farnesylation motif of HRAS with N-terminus EGFP.DepositorInsertHomo sapiens Harvey rat sarcoma (HRAS Human)
UseTagsGFPExpressionMammalianMutationcontains the region from 825-857 of HRAS as a far…PromoterCMVAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only