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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 305 results for: Gnas

Showing: 1 - 20 of 305 results

  1. Synthetic Biology - Sensing and Signaling

    Type
    Collection
    ...for Sensing and Signaling.... Plasmid Collections Synthetic Biology Sensing and Signaling Synthetic Biology...Biology: Sensing and Signaling SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog...Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection of synthetic...communications and environmental sensing. Sensing and Signaling Plasmids Search the table by keyword or sort by...

  2. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...the kinases are located, their signal strength, and how long their signals last all impact transduction ...feedback loops of signaling pathways Negative feedback loops are a common feature of signal transduction pathways...Signal transduction pathways are a lot like cell phone networks. Protein kinases deliver messages to ...transduction of the message. To study signaling pathways, scientists frequently use growth factors or serum to stimulate... but there can be a lot of static since other signaling networks can also be non-specifically activated...activated and, like the game of telephone, often the signal must be transmitted by intermediate messengers. ...kinase inhibitors to alter Raf1-MEK-ERK pathway signaling by using psMEK1 and an ERK kinase translocation...

  3. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ... in decreased BRET2 signal. With increased concentration of drug, the BRET2 signal decreases. The...partial understanding of how GPCRs signal through G protein signal transducers. The TRUPATH collection...receptors (GPCRs), receptors that play many roles in signaling processes including smell, taste, sight, inflammation...to interrogate 14 of the 16 non-visual G protein signal transducers (collectively known as the transducerome...heterotrimeric G protein. This complex converts a signal received by a GPCR on the cell’s surface into action... result in the initiation of a vast number of signaling cascades, inducing different cellular responses...subunit from the Gβγ dimer, activating downstream signaling pathways.  This dissociation can be measured using...

  4. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...conserved eukaryotic polyadenylation signal directs cleavage at the cleave signal and addition of a poly-A tail...elements, such as the polyadenylation or poly(A) signal. While many studies focus on promoter strength ...?curid=17554393   Use of mammalian poly(A) signals in combination with certain viral packaging systems...WPRE (5) and CTE (6), and/or use weaker poly(A) signals like BGH.  References Peters JM, Vangeloff AD...element of the simian virus 40 late polyadenylation signal by using in vitro analyses. Molecular and Cellular...Burns JE, Maitland NJ. An Internal Polyadenylation Signal Substantially Increases Expression Levels of Lentivirus-Delivered...

  5. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ...identification of signaling pathways with potential to confer anticancer drug resistance." Science signaling 7.357...emerging theme is that they do so by activating signaling pathways controlling properties like growth, survival...kits/sabatini-wood-cancer-pathway-orfs/ Finding signaling pathways involved in drug resistance Wood wanted...exactly that. As described in the journal Science Signaling in December 2014, he and his colleagues at Duke... Institute and MIT, started with a list of 17 signaling pathways that are frequently implicated in cancer...

  6. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    ...the extracellular signaling-regulated kinase (ERK) signaling cascade. This signaling is a lot like a game...control of signaling pathways. This is particularly exciting because using light to control signaling provides...that bind cell signaling receptors would offer more levers to pull to control signaling pathways. For ...as well as reversibly regulation of endogenous signaling activity in cells.  Let’s shed some light on the...for protein purification and controlling cell signaling. Key components of OptoNBs OptoNBs have two key...surface of a bead.  Using OptoNBs to control cell signaling The second application for OptoNBs takes us into...pharmacological or genetic manipulation of the signaling pathway.  As a proof-of-principle, the team created...

  7. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    ...CRISPR array in the resulting Ignavibacteriae phylum genome. IgnaviCas9 is a hyperthermostable Cas9 CRISPR... like GeoCas9 and ThermoCas9, IgnaviCas9 is a type II-C Cas9. IgnaviCas9 is most similar to a type II-... temperature is 30°C. Find IgnaviCas9 here! What can you do with IgnaviCas9? Why would a thermostable ...projects were launched in the Quake Lab. Identifying IgnaviCas9, a hyperthermostable Cas9 This year it was wonderful...assembled genomes, but also led to the discovery of IgnaviCas9, a new CRISPR enzyme from a sample from Mound...CRISPR nuclease that is active up to 100°C.  The IgnaviCas9 protein is 1,241 amino acids long, has optimal...protocol steps can be saved by flexibly deploying IgnaviCas9 without concern about its stability under harsh...

  8. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...level has different safety requirements. BSL-1 is designated for those working with microbes that don’t cause...before and after working in the lab. Ensure that a designated chemical waste accumulation site is present, ...(MSDS). While You Work Dispose of sharps in a designated sharps container. Sharps include anything that... drain. Solid BSL-2 waste can be collected in designated biohazardous waste containers that can be autoclaved...

  9. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ... background signal that is most evident during initial PCR cycles. This background signal must be removed... of primer dimers which can increase background signal and alter the Ct values of your samples. Quality...

  10. AAV ddPCR Titration

    Type
    Protocol
    ...Denaturation 95 0.5 2 50 Annealing/Extension 60 1 2 50 Signal Stabilization 98 10 2 1 Hold 4 ∞ 2 1 After PCR ...QuantaSoft software to set up a new plate layout. Designate the sample name, experiment type, supermix type...

  12. Lentivirus ddPCR Titration

    Type
    Protocol
    ...QuantaSoft software to set up a new plate layout. Designate the sample name, experiment type, supermix type...

  14. Antibody Guide

    Type
    Collection
    ...antibody and conjugate chosen. Signal Amplification The amount of signal needed from a specific antibody...abundant target, it obscures fainter signals. Fluorophores can emit signals that overlap with each other. Choose... Other Affinity Reagents Handling Visualization Signal Amplification Application Overview Quantification...Fc region. This allows for the amplification of signals by having multiple secondary antibodies binding...sdAbs is usually achieved by conjugation of a signaling molecule directly to the sdAb, or by using a secondary... incorporated in the sdAb. sdAbs can have weak signals due to their monovalent nature. Darpins - Darpins...single arm of the Y). They can be conjugated to signaling molecules and are often used in clinical applications...

  15. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... retention signal moxGFP Erik Snapp 68126 ERoxBFP Endoplasmic Reticulum ER retention signal oxBFP Erik...structures. ID Plasmid Structure Targeting Gene/Signal Fluorescent Protein PI 98875 3xnls-mNeonGreen Nucleus...-derived (1-22 aa) import sequence and COX VIII signal peptide dL5 FAP; mCerulean3 Marcel Bruchez 44385...Lck-mTurquoise2 Plasma Membrane Lck N-terminal lipidation signal mTurquoise2 Dorus Gadella 98821 Lck-mScarlet-I ...Lck-mScarlet-I Plasma Membrane Lck N-terminal lipidation signal mScarlet-I Dorus Gadella 50057 pLYS1-FLAG-MitoGFP-HA...49151 mito-BFP Mitochondria mitochondrial targeting signal (COX4) TagBFP Gia Voeltz 55102 mCherry-Mito-7 Mitochondria...pTDpelB-C_sfYFPTwinStrep Periplasmic space PelB signal sequence YFP Thorben Dammeyer 54520 mCherry-Peroxisomes...

  16. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...sensing region of the construct leads to a change in signal of a circularly permuted fluorescent protein (cpFP...database includes many fluorescent biosensors that signal the presence of specific biomolecules, the activity...design, biosensors can enable investigation of a signaling pathway or measurement of a biomolecule while ...Conditions and constraints for astrocyte calcium signaling in the hippocampal mossy fiber pathway. Neuron...Single-color, ratiometric biosensors for detecting signaling activities in live cells. eLife. 2018 Jul 3;7....single-wavelength fluorescent sensor for cyclic AMP. Sci Signal. 2018 Mar 6;11(520). pii: 11/520/eaah3738. Justin...biosensors for sensitive and multiplexed detection of signalling activities. Nat Cell Biol. 2018 Sep 24. pii: ...

  17. mTOR Pathway

    Type
    Collection
    ...pro-proliferative and anti-apoptotic signals. mTORC2, which appears to signal upstream of Akt, also promotes...Addgene's plasmids for the mTOR pathway. enhanced signaling of mTOR, a key metabolic regulator, correlates...better-characterized mTORC1 responds to various signals, including amino acids, stress, oxygen, energy ...levels, and growth factors. mTORC1 integrates these signals to determine the nutritional state of the cell ...cytoskeletal organization. Cancer Impact Enhanced signaling through either mTORC1 or mTORC2 correlates with...from David Sabatini & Kris Wood) References mTOR signaling in growth control and disease. Laplante M, Sabatini...

  18. Immunology Research Plasmids and Resources

    Type
    Collection
    ... genes involved in innate and adaptive immune signaling and cell-cell communication....II molecules found on the surface of APC. BCR Signaling B cells mediate humoral immunity through the secretion... receptor (BCR). The strength and duration of signaling controls the fate of B cells and can lead to the...trigger. Interferons The interferon family of signaling proteins consists of Type I, Type II, and Type... 60 interleukins have been described. NK Cell Signaling Natural killer (NK) cells can kill cells that ...infected cell membrane, triggering cell death. TCR Signaling The T cell receptor (TCR) engages with antigen...Engagement of the TCR by an MHC molecule presenting its cognate antigen along with co-stimulation activates the...

  19. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...stimulation. GPCR signaling GPCR signaling is a complicated process involving many downstream signaling mechanisms...activating entire signaling pathways, but what if you’re interested in studying signaling pathways? Here’... secondary signaling pathways with light. This is achieved by coupling endogenous signaling molecules ...When exposed to light, they activate downstream signaling pathways that can change both the membrane potential...neurons without needing to consider secondary signaling pathways. There are many different opsin (natural...cellular function through multiple intra-cellular signaling cascades. Optical methods for controlling GPCR...light to control GPCR activation and downstream signaling cascades (reviewed in Abreu & Levitz, 2020), including...

  20. Cancer Research Plasmids and Resources

    Type
    Collection
    ...of plasmids for cancer research, organized by signaling pathway. ... large variety of resources for use in cancer signaling studies, including mutant, knockdown, and overexpression...below organizes the plasmids and resources by signaling pathway, focusing on the main pathways involved.... These pathways are important to normal cell signaling and physiology, and, when dysregulated, can enable... mTOR mTOR is a protein kinase that anchors a signaling network for transitioning between anabolic and... Labs containing cDNAs for studying oncogenic signaling pathways. ClonTracer Library : Barcoding library...
Showing: 1 - 20 of 305 results