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Showing: 5601 - 5650 of 17798 results

  14. pCRISPaint-mScarlet-F-Ova-PuroR [M1G]

    Plasmid
    #171813
    Purpose
    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mScarlet-F-Ova-T2A-PuroR
    Use
    Gene tagging
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Available Since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    171813-plasmid-map-sequence-id-338044

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  26. TRE-RXRa-Myc; pGK-H2B-RFP

    Plasmid
    #242887
    Purpose
    For lentiviral overexpression of Myc-tagged human RXRa coupled to H2B-RFP (in the opposite direction)
    Depositor
    Elaine Fuchs
    Article
    Stewart et al Nature. 2024 Sep;633(8029):407-416. doi:
    Insert
    Rxra (RXRA Human)
    Use
    Lentiviral
    Tags
    H2B-mRFP
    Expression
    Mammalian
    Mutation
    Human RXRa cDNA was PCR-amplified from pSV-Sport-…
    Promoter
    Tetracycline response element upstream of a minim…
    Available Since
    Aug. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    242887-plasmid-map-sequence-id-484988

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  31. pαH-S-GSAS-2P-N317P

    Plasmid
    #196378
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein with mutation N317P with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987
    Depositor
    Priyamvada Acharya
    Article
    Manrique et al iScience. 2023 Jan 20;26(1):105855. doi:
    Insert
    S-GSAS-2P-N317P (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV 3C cleavage site (before tags) C terminal on …
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    March 24, 2023
    Availability
    Academic Institutions and Nonprofits only

  32. pCRISPaint-mNeon-F-gB-PuroR [M1G]

    Plasmid
    #171803
    Purpose
    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mNeonGreen-FLAG-gB-T2A-PuroR
    Use
    Gene tagging
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Available Since
    Sept. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    171803-plasmid-map-sequence-id-341955

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  33. pCRISPaint-mScarlet-F-gB-PuroR [M1G]

    Plasmid
    #171812
    Purpose
    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and puromycin resistance cassette [p.M1
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mScarlet-F-gB-T2A-PuroR
    Use
    Gene tagging
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Available Since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171812-plasmid-map-sequence-id-337985

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  34. pCRISPaint-mScarlet-F-mgp100-PuroR [M1G]

    Plasmid
    #171811
    Purpose
    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, murine gp100 CD8+ T cell epitope [AA: 25-33 (EGSRNQDWL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mScarlet-F-mgp100-T2A-PuroR
    Use
    Gene tagging
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Available Since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171811-plasmid-map-sequence-id-337983

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  35. pCRISPaint-mScarlet-F-hgp100-PuroR [M1G]

    Plasmid
    #171810
    Purpose
    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mScarlet-F-hgp100-T2A-PuroR
    Use
    Gene tagging
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Available Since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171810-plasmid-map-sequence-id-337982

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  36. pCRISPaint-mNeon-F-Ova-PuroR [M1G]

    Plasmid
    #171804
    Purpose
    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mNeonGreen-FLAG-Ova-T2A-PuroR
    Use
    Gene tagging
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Available Since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171804-plasmid-map-sequence-id-337975

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  37. pHD-dsRed-attP_dCrk_flankingHRs

    Plasmid
    #131141
    Purpose
    Contains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).
    Depositor
    Mark Peifer
    Article
    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.
    Inserts
    Crk Upstream Homology Region (Crk Fly)
    Crk Downstream Homology Region (Crk Fly)
    Use
    CRISPR and Cre/Lox; Homology directed repair temp…
    Available Since
    Oct. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    131141-plasmid-map-sequence-id-258858

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Showing: 5601 - 5650 of 17798 results