We narrowed to 4,045 results for: golden gate

Showing: 2241 - 2260 of 4045 results

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L1p3_barley_sgRNA_scaffold

Plasmid

#231030

Purpose

Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 3

Depositor

Wilma van Esse

Article

Winkelmolen et al bioRxiv 2024.08.22.609225

Insert

Scaffold for Cas9 mediated CRISPR

Use

CRISPR

Expression

Bacterial

Promoter

TaU6

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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Zdk3-VPR

Plasmid

#231572

Purpose

Expresses components of the LOOMINA optogenetic transcriptional control system.

Depositor

Dominik Niopek

Article

Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

Insert

Zdk3-VPR, Ef1a driven

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA26_sgRNA-A0_M13ps

Plasmid

#231413

Purpose

M13 phagemid constitutively expressing sgRNA-A0. sgRNA-A0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI).

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Insert

sgRNA-A0

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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PAF24

Plasmid

#223172

Purpose

Expresses VC83_00191-yeGFP under control of CTR1 promoter and terminator

Depositor

Ryan Peterson

Article

Anne et al J Fungi (Basel). 2024 Oct 21;10(10):729.

Insert

Copper Transporter

Tags

StreptagII and yeGFP

Expression

Yeast

Promoter

saccharomyces cerevisiae CTR1

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pHW622

Plasmid

#229868

Purpose

Entry vector with reverse FRT3 (rFRT3) in slot 7 (for SapTrap assembly)

Depositor

Han Wang

Article

Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

Insert

reverse FRT3 (rFRT3)

Expression

Worm

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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MbCas12a_K487_GPG_GR2

Plasmid

#231577

Purpose

Encodes a cortisol inducible variant of MbCas12a

Depositor

Dominik Niopek

Article

Wolf et al bioRxiv 2024.12.04.626757

Insert

MbCas12a with a GR2 insertion behind K487

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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cpLOV2-Gal4

Plasmid

#231569

Purpose

Expresses components of the LOOMINA optogenetic transcriptional control system.

Depositor

Dominik Niopek

Article

Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

Insert

cpLOV2-Gal4

Expression

Mammalian

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_Zdk3-VPR

Plasmid

#231573

Purpose

Expresses components of the LOOMINA optogenetic transcriptional control system.

Depositor

Dominik Niopek

Article

Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

Insert

Zdk3-VPR, CMV driven

Expression

Mammalian

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCMK83

Plasmid

#226793

Purpose

Integrates slr0214 (HIP1 methyltransferase) into gidB-atpI locus of E. coli DH10B

Depositor

David Nielsen

Article

Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

Insert

slr0214 (slr0214 Synechocystis sp. PCC 6803)

Expression

Bacterial

Promoter

Tetracycline resistance cassette promoter (consti…

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pRDB_268

Plasmid

#231112

Purpose

SpG-Cas9

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Insert

Cas9-SpG

Use

CRISPR and Lentiviral

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGGA016

Plasmid

#228231

Purpose

Level 0 for 5xOp (GreenGate A module)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

5xOp

Use

Synthetic Biology

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW111111

Plasmid

#222456

Purpose

Level 1 acceptor, Position 3 containing CBS4-miniDFR::LacZ::nosT.

Depositor

Chih-Hang Wu

Article

Chiang et al bioRxiv 2024.03.23.586378

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Mutation

no

Promoter

CBS4-miniDFR (Solanum lycopersicum)

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW111112

Plasmid

#222457

Purpose

Level 1 acceptor, Position 3 containing CBS4-miniDFR::LacZ::nosT.

Depositor

Chih-Hang Wu

Article

Chiang et al bioRxiv 2024.03.23.586378

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Mutation

no

Promoter

CBS4-miniDFR

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW00015

Plasmid

#222446

Purpose

Level 0, C terminal tag module (TTCG-GCTT) containig VP64-NLS.

Depositor

Chih-Hang Wu

Article

Chiang et al bioRxiv 2024.03.23.586378

Insert

VP64-NLS

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW91069

Plasmid

#222462

Purpose

Level 1 acceptor, Position 3 containing CBS4-miniDFR::mCherry2-HyP5SM-LacZ::nosT.

Depositor

Chih-Hang Wu

Article

Chiang et al bioRxiv 2024.03.23.586378

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Mutation

no

Promoter

CBS4-miniDFR (Solanum lycopersicum)

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW00014

Plasmid

#222445

Purpose

Level 0, C terminal tag module (TTCG-GCTT) containing VP16-NLS.

Depositor

Chih-Hang Wu

Article

Chiang et al bioRxiv 2024.03.23.586378

Insert

VP16-NLS

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW110034

Plasmid

#222443

Purpose

Level 0, N-terminal tag module (CCAT-AATG) containing LoxP-mCherry.

Depositor

Chih-Hang Wu

Article

Chiang et al bioRxiv 2024.03.23.586378

Insert

LoxP-mCherry

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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PTet-F30-Pepper aptamer-UTR1-mTurq2

Plasmid

#227645

Purpose

pTet-driven expression of F30-scaffolded Pepper aptamer and mTurquoise2 for quantifying cell-free RNA and protein synthesis. The mTurq2 has a GGGGS linker followed by a His6x tag.

Depositor

Richard Murray

Article

Kapasiawala et al bioRxiv 2023.03.22.533877

Insert

F30-scaffolded Pepper aptamer and mTurquoise2 (codon optimized for E. coli B using IDT Codon Optimizer)

Use

Synthetic Biology

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL2142 MTK3-sfGFP

Plasmid

#226661

Purpose

MTK3 part containing the coding sequence of sfGFP

Depositor

Chang Liu

Article

Loveless et al bioRxiv 2021.11.05.467507

Insert

sfGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

none

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM034_P7_3"Lys3

Plasmid

#216459

Purpose

3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

3''lys3

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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