We narrowed to 3,800 results for: golden gate

1. pMVRha

   Plasmid

   #229553

   Purpose

   Broad-host range plasmid (pBBR1), contains an oriT (RP4) for conjugation in kanamycin sensitive non-model bacteria. The GOI can be cloned under the control of a rhamnose-inducible promoter using BsaI.

   Depositor

   Sandy Schmidt

   Article

   Vajente et al ACS Synth Biol. 2024 Oct 31. doi: 10.102

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDV052

   Plasmid

   #191825

   Purpose

   Plasmid for the transformation of the cyanobacterium Arthrospira platensis at the KmR locus. No promoter. No terminator. Golden Gate compatible. Selection: in A. platensis: KmR; E. coli: AmpR

   Depositor

   Jim Roberts

   Article

   Tabakh et al STAR Protoc. 2023 Feb 8;4(1):102087. doi

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   Jan. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Tad-CBE(CCG3)

   Plasmid

   #235074

   Purpose

   Expresses Tad-CBE(CCG3) in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE(CCG3)-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, bpNLS and bpNLS

   Expression

   Mammalian

   Available Since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCHW101020

   Plasmid

   #222454

   Purpose

   Level 1, Position 2 containing 35S::CUP2-p65::NOS.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Insert

   35S::CUP2-p65::NOS (CUP2 )
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   no

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRAM29

   Plasmid

   #234315

   Purpose

   G264A Cat-RNA U1367, Catalytically dead ribozyme control

   Depositor

   James Chappell

   Article

   Kalvapalle et al Nat Biotechnol. 2025 Mar 18. doi: 10.103

   Insert

   Splicing-deficient mutant of ribozyme
   

   Use

   Synthetic Biology

   Promoter

   P. CymRC

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDN-Cherry-sgRNA

   Plasmid

   #221387

   Purpose

   episomal (HygR), with constitutive mCherry (Psmyc) and AHT-inducible sgRNA cloning site, including Golden Gate for multiple sgRNAs

   Depositor

   Deborah Hung

   Article

   Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523

   Insert

   none, cloning vector for introduction of targeting sequences
   

   Use

   CRISPR

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJJB1439

   Plasmid

   #218589

   Purpose

   Express peroxisomal Idi1p, Erg20(F96W,N127W)p, and GESp to convert IPP to geraniol

   Depositor

   John Dueber

   Article

   Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

   Insert

   GES
   

   Tags

   ePTS1

   Expression

   Yeast

   Mutation

   Erg20(F96W, N127W)

   Available Since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pICH-chl-roGFP2-synORP1

   Plasmid

   #191701

   Purpose

   Ti plasmid pICH86988 harbouring chl-roGFP2-synORP1 for H2O2 detection in the plastids of plant cells.

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

   Insert

   chl-roGFP2-synORP1
   

   Use

   Binary agrobacterium ti plasmid

   Expression

   Plant

   Promoter

   CaMV 35S promoter

   Available Since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pICH-nuc-roGFP2-synORP1

   Plasmid

   #191759

   Purpose

   Ti plasmid pICH86988 harbouring nuc-roGFP2-synORP1 for H2O2 detection in the nuclei of plant cells.

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

   Insert

   nuc-roGFP2-synORP1
   

   Use

   Binary agrobacterium ti plasmid

   Expression

   Plant

   Promoter

   CaMV 35S

   Available Since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJM456 EF1alpha EBFP2-P2A-BlastR (TUPV7)

    Plasmid

    #139236

    Purpose

    mMoClo TUPV7, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    EBFP2-P2A-Blasticidin S Deaminase
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Tad-CBE(GCC3)

    Plasmid

    #235078

    Purpose

    Expresses Tad-CBE(GCC3) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(GCC3)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, bpNLS and bpNLS

    Expression

    Mammalian

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. Tad-CBE(GCG4)

    Plasmid

    #235080

    Purpose

    Expresses Tad-CBE(GCG4) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(GCG4)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, bpNLS and bpNLS

    Expression

    Mammalian

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. BRD4dN-mCherry-SspB

    Plasmid

    #227387

    Purpose

    Condensate module, light-inducible oligomerization IDR

    Depositor

    Clifford Brangwynne

    Article

    Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

    Insert

    BRD4 IDR
    

    Use

    Lentiviral

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ131B

    Plasmid

    #69281

    Purpose

    Golden Gate entry vector; 1st gRNA under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSEM246 - mosTI - unc-119 - MCS

    Plasmid

    #182343

    Purpose

    Empty cloning vector for mosTI(unc-119). MCS or Golden Gate (BsaI: tgcc - MCS - gagc)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Bxb1-GA_CAG-mStayGold

    Plasmid

    #229802

    Purpose

    Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mStayGold
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ131C

    Plasmid

    #69284

    Purpose

    Golden Gate entry vector; 1st gRNA under OsU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQ132C

    Plasmid

    #69285

    Purpose

    Golden Gate entry vector; 1st gRNA under OsU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. Tad-CBE(CCC5)

    Plasmid

    #235073

    Purpose

    Expresses Tad-CBE(CCC5) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(CCC5)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, bpNLS and bpNLS

    Expression

    Mammalian

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Tad-CBE(TCT1)

    Plasmid

    #235065

    Purpose

    Expresses Tad-CBE(TCT1) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(TCT1)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, bpNLS and bpNLS

    Expression

    Mammalian

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge