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Plasmid#87348PurposeD435C/E945C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, lobe closure FRET constructDepositorInsertCas9
UseTagsHis-MBPExpressionBacterialMutationC80S/D435C/C574S/E945CPromoterT7Available sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTMS1
Plasmid#87799PurposeFor constructing MBP-SUMO-fusion proteins with N-terminal His tag and C-terminal strep II tag using "blunt-end" cloning.DepositorTypeEmpty backboneUseTags6xHis and Strep IIExpressionBacterialMutationPromoterAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSHS248
Plasmid#87354PurposeS867C/N1054C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-2 FRET constructDepositorInsertCas9
UseTagsHis-MBPExpressionBacterialMutationC80S/C574S/S867C/N1054CPromoterT7Available sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTMS2
Plasmid#87800PurposeFor constructing MBP-SUMO-fusion proteins with N-terminal StrepII tag and C-terminal His tag using "blunt-end" cloning.DepositorTypeEmpty backboneUseTags6xHis and Strep IIExpressionBacterialMutationPromoterAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a:GST-AvrPWT4
Plasmid#237522PurposeRecombinant protein expressionDepositorInsertAvrPwt4
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a:His-RWT4Ca
Plasmid#237519PurposeRecombinant protein expressionDepositorInsertRwt4Ca
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a:GST-VirPWT4
Plasmid#237523PurposeRecombinant protein expressionDepositorInsertVirPwt4
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
polySIM
Plasmid#221068PurposeExpression of polySIM protein under T7 promoterDepositorInsert10 repeats of SUMO interacting motif (SIM) from PIASx each separated by (GGS)4
UseTagsHis and MBPExpressionBacterialMutationPromoterunknownAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
2M-T-Mce3R_K262A
Plasmid#225728PurposeTo express Mce3R mutant K262A in E. coliDepositorInsertmce3R
UseTags6xHis-MBPExpressionBacterialMutationK262APromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
2M-T_Mce3R
Plasmid#225726PurposeTo express Mce3R in E. coliDepositorInsertmce3R
UseTags6xHis-MBPExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only