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pET28a(+)-HisTim12_CtoA

Plasmid

#170281

Purpose

Expresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

Depositor

Paul Schanda

Article

Weinhaupl et al Structure. 2021 May 3. pii: S0969-2126(2

Insert

yeast Tim12 (TIM12 Budding Yeast)

Tags

TEV-cleavable N-terminal His tag (on backbone)

Expression

Bacterial

Mutation

mutated two non-conserved Cys to Ala

Available Since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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pUE1-TSP-ORF68 R14A/K310A

Plasmid

#162655

Purpose

Expresses ORF68 R14A/K310A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Mutation

Amino acids R14 and K310 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLJM1-zeo-2xStrep-ORF68 C52A

Plasmid

#162660

Purpose

Lentiviral vector for stable expression of N-terminal 2xStrep-tagged ORF68 C52A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Use

Lentiviral

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid C52 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-ORF68 K174A/R179A/K182A

Plasmid

#162639

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 K174A/R179A/K182A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acids K174, R179, and K182 mutated to alani…

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pUE1-TSP-ORF68 K435A

Plasmid

#162651

Purpose

Expresses ORF68 K435A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Mutation

Amino acid K435 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pUE1-TSP-ORF68 K450A/R451A

Plasmid

#162653

Purpose

Expresses ORF68 K450A/R451A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Mutation

Amino acids K450 and R451 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG-altTAG

Plasmid

#158132

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Expression

Yeast

Mutation

Cloned in a TAG codon at the first serine residue…

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG-altTAG2

Plasmid

#158134

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Expression

Yeast

Mutation

Cloned in a TAG codon at the second serine residu…

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG-altTAG3

Plasmid

#158136

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Expression

Yeast

Mutation

Cloned in a TAG codon at the third alanine residu…

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG-altTAG4

Plasmid

#158137

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Expression

Yeast

Mutation

Cloned in a TAG codon at the third serine residue…

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pMJB1-RPL1B-Cl

Plasmid

#160430

Purpose

Expresses "cre-less" yeast RPL1B tagged with mCherry under the GAL1 promoter

Depositor

Gary Stormo

Article

Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi:

Insert

RPL1B

Tags

mCherry

Expression

Yeast

Mutation

Removed introns, shuffled codons to vary nucleoti…

Promoter

S. cerevisiae GAL1

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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Y365A or A365Y inducible rescue

Plasmid

#131323

Purpose

gRNA to generate i-WT-r or i-MT-r systems

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

Y365A or A365Y inducible rescue

Use

CRISPR

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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HoxC-downstream-5xTetO Donor

Plasmid

#131341

Purpose

Donor template to insert 5xTet operator sequence into downstream of HoxC cluster.

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

HoxC-downstream-5xTetO Donor

Use

CRISPR and Mouse Targeting

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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5xtetO HoxC 3'

Plasmid

#131340

Purpose

gRNA to insert 5xTet operator sequence into downstream of HoxC cluster.

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

HoxC-downstream-gRNA

Use

CRISPR

Expression

Mammalian

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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MTF2_KO_ex11_gRNA-4

Plasmid

#131334

Purpose

gRNA to knockout MTF2. This gRNA is also used in Haojie Li et al. Nature 2017. It targets exon 11 of MTF2.

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

MTF2_KO_ex11_gRNA

Use

CRISPR

Expression

Mammalian

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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LMX1B-gRNA-N-del

Plasmid

#131333

Purpose

gRNA to delete a nucleation site near Lmx1b. Use with LMX1B-gRNA-C-del

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

LMX1B-gRNA-N-del

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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HoxD del ds1

Plasmid

#131338

Purpose

gRNA to delete a nucleation site near HoxD. Use with HoxD del us1

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

EVX2-gRNA-C-del

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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SKOR1-gRNA-C-del

Plasmid

#131332

Purpose

gRNA to delete a nucleation site near Skor1. Use with SKOR1-gRNA-N-del

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

SKOR1-gRNA-C-del

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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SKOR1-gRNA-N-del

Plasmid

#131331

Purpose

gRNA to delete a nucleation site near Skor1. Use with SKOR1-gRNA-C-del

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

SKOR1-gRNA-N-del

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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HoxD del control ds

Plasmid

#131336

Purpose

gRNA to delete control region near HoxD. Use with HoxD del control us

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

CTRL-gRNA-C-del

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only