We narrowed to 17,121 results for: URE

1. p6XHis_NLS-SaCas9

   Plasmid

   #101086

   Purpose

   Express SaCas9 in bacteria with a 6xHis tag for purification

   Depositor

   Rick Tarleton

   Article

   Soares Medeiros et al MBio. 2017 Nov 7;8(6). pii: e01788-17. d

   Insert

   CRISPR-associated protein Cas9/Csn1
   

   Use

   CRISPR

   Tags

   TrxA-6xHis-STag

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available since

   Dec. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX neo PFKL-GFP

   Plasmid

   #138289

   Purpose

   Mammalian Expression, Lentiviral. Expression of human phosphofructokinase, liver isoform PFKL-GFP fusion protein

   Depositor

   Gaudenz Danuser

   Article

   Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

   Insert

   PFKL (PFKL Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. VP12

   Plasmid

   #72247

   Purpose

   Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A and Q926A

   Promoter

   CMV

   Available since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX6P1-HA-MEK1

   Plasmid

   #53159

   Purpose

   Expresses GST-HA tagged human MEK1 from bacterial expression vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   MEK1 (MAP2K1 Human)
   

   Use

   Tags

   GST and HA

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. BPK4410 - human expression plasmid for SpCas9 Cluster 1 (HypaCas9)

   Plasmid

   #101178

   Purpose

   Human expression plasmid for SpCas9 Cluster 1 variant (HypaCas9): CMV-T7-hSpCas9-Cluster1(N692A, M694A, Q695A, H698A)-NLS(SV40)-3xFLAG

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster1(N692A/M694A/Q695A/H698A)
   

   Use

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   N692A/M694A/Q695A/H698A

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti CMV SNAP MDC1

   Plasmid

   #227375

   Purpose

   SNAP–MDC1 expression

   Depositor

   David Pellman

   Article

   Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

   Insert

   MDC1, mediator of DNA damage checkpoint 1 (MDC1 Human)
   

   Use

   Lentiviral

   Tags

   SNAP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAnc80L65

   Plasmid

   #68837

   Purpose

   Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct. Note that an improved version is available: Addgene plasmid# 92307. See comments for details

   Depositor

   Luk Vandenberghe

   Article

   Zinn et al Cell Rep. 2015 Jul 28. pii: S2211-1247(1

   Insert

   Ancestral AAV Capsid Anc80L65
   

   Use

   AAV and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Rep2

   Available since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX neo ACTN1-GFP

   Plasmid

   #138293

   Purpose

   Mammalian Expression, Lentiviral. Expression of human alpha-actinin-1-GFP fusion protein

   Depositor

   Gaudenz Danuser

   Article

   Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

   Insert

   ACTN1 (ACTN1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSECC

   Plasmid

   #60820

   Purpose

   3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre

   Depositor

   Tyler Jacks

   Article

   Sanchez-Rivera et al Nature. 2014 Oct 22. doi: 10.1038/nature

   Inserts

   Cas9
   Cre
   

   Use

   CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   BsmBI site eliminated by C->A; E308G

   Promoter

   EFS and EFS (after Cas9-2A)

   Available since

   Nov. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. (565) pAAV Alb-AAT KRAB-SadCas9 U6-gSTOP

    Plasmid

    #163030

    Purpose

    Expression of CRISPRi with gSTOP gRNA

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9
    

    Use

    AAV

    Tags

    KRAB, SV40 pA, and myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Alb-AAT

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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