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pGWB660-CYCD3;1

Plasmid

#140751

Purpose

Inducing plant cells to enter mitosis

Depositor

Bo Liu

Article

Xu et al New Phytol. 2019 Nov 2. doi: 10.1111/nph

Insert

Cyclin D3;1 (CYCD3;1 Mustard Weed)

Use

Tags

TagRFP

Expression

Plant

Mutation

Promoter

CaMV 35 promoter

Available since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pHRm-GFP(1-10)-VP64-NLS

Plasmid

#70228

Purpose

Expresses GFP(1-10)-VP64-NLS in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

GFP(1-10)-VP64-NLS

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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APEX2-csGBP

Plasmid

#108874

Purpose

Expression of APEX2 fused to a conditionally stable nanobody against GFP

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2-csGBP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAGT10446

Plasmid

#192353

Purpose

L0 MoClo-compatible GoldenGate-module of the 35S terminator (t35S) with stop

Depositor

Tom Schreiber

Article

Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

Insert

TTCG_Stop-t35S_CGCT

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pV1F(Spec)

Plasmid

#205086

Purpose

Prokaryotic expressing of AtVIP1 fused with a sequence encoding a T4SS translocation tag in A. tumefaciens.

Depositor

Suomeng Dong

Article

Wang et al PLoS Pathog. 2023 Apr 21;19(4):e1011346.

Insert

AtVIP1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pSEM371 - ce-unc-119 array integration

Plasmid

#182640

Purpose

Co-injection fragment for extra-chromosomal array integration at ce-unc-119 locus

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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H2B-mCherry

Plasmid

#108880

Purpose

Expression of human histone2B fused to mCherry (nuclear marker)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

H2B-mCherry

Use

Can be used for mammalian expression or in vitro …

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-hCDC6p-Venus

Plasmid

#212665

Purpose

Express Venus under CDC6 promoter

Depositor

Tobias Meyer, Yumi Konagaya

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

hCDC6p-Venus

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hCDC6p

Available since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pSPORT-RREB1

Plasmid

#41145

Depositor

Joshua Mendell

Article

Kent et al Oncogene. 2012 Jul 2. doi: 10.1038/onc.2

Insert

RREB-1 (RREB1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pFZ19

Plasmid

#36184

Depositor

Daniel Voytas

Article

Zhang et al Proc Natl Acad Sci U S A. 2010 Jun 29. 1

Type

Empty backbone

Use

Plant t-dna vector

Tags

Expression

Plant

Mutation

Promoter

XVE

Available since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only

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GFP-Ago4

Plasmid

#21536

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago4 (AGO4 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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Human RNF144A (16-228)

Plasmid

#110752

Purpose

Bacterial Expression of RNF144A RBR domain

Depositor

David Komander

Article

Michel et al Mol Cell. 2017 Oct 5;68(1):233-246.e5. d

Insert

RNF144A (RNF144A Human)

Use

Tags

N-His6-GST-3C

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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mRuby2-C1

Plasmid

#54768

Purpose

Localization: C1 Cloning Vector, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Type

Empty backbone

Use

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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LeGO-S2

Plasmid

#85211

Purpose

Lentiviral vector expressing T-Sapphire fluorescent protein driven by SFFV promoter

Depositor

Boris Fehse

Article

Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

Insert

T-Sapphire

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Jan. 18, 2017

Availability

Academic Institutions and Nonprofits only

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PlayBack-Ef1a-Bamh1

Plasmid

#203308

Purpose

For expression of eukaryotic genes of interest with Ef1a promoter with Bamh1 as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pME-secAnxaV-NS

Plasmid

#67718

Purpose

Gateway entry clone containing secreted human AnxaV with no stop codon

Depositor

Rob Parton

Article

Morsch et al Front Cell Neurosci. 2015 Aug 31;9:321.

Insert

secAnxaV-NS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Camui-CR

Plasmid

#40256

Depositor

Michael Lin

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Camuiα-CR

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only

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p3E-TurboID

Plasmid

#166570

Purpose

Multisite Gateway 3' entry clone for fusions onto the N-terminus of TurboID. Parton lab clone JSS

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

TurboID

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pSIL-eGFP

Plasmid

#52675

Purpose

shRNA silencing plasmid with eGFP transfection marker

Depositor

Johannes Hell

Article

Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

Type

Empty backbone

Use

RNAi

Tags

CMV-EGFP

Expression

Mutation

Promoter

CMV and U6

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-YFP-PKD2

Plasmid

#31821

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2009 Sep 4. 284(36):24653-6

Insert

YFP-Protein Kinase D2 (PRKD2 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
Inducible tdTomato

Plasmid

#183718

Purpose

tdTomato expression from dox-inducible promoter; plasmid encodes the TetR inducer

Depositor

Jeffrey Gerst

Article

Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.75

Inserts

tdTomato
rTetR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE3GV and UbiC

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-YFP-PKD1

Plasmid

#31527

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2009 Sep 4. 284(36):24653-6

Insert

YFP-HA-Protein Kinase D (PRKD1 Human)

Use

Tags

HA and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2011

Availability

Academic Institutions and Nonprofits only
SS9_RNA

Plasmid

#71656

Purpose

SS9-targeting gRNA for co-transformation with pSS9

Depositor

Ryan Gill

Article

Bassalo et al ACS Synth Biol. 2016 Apr 22.

Insert

SS9 gRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pEMS1982

Plasmid

#49113

Purpose

AAV plasmid with CLDN5 (Ple261) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Baumann et al Exp Eye Res. 2019 Jul 16:107728. doi: 10

Insert

ssAAV-Ple261-iCre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CLDN5

Available since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pME-mTagBFP

Plasmid

#75148

Purpose

TagBFP (blue) fluorophore for use with 5’ promoters.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

mTagBFP

Use

Middle entry vector for multisite gateway three f…

Tags

Expression

Mutation

Promoter

n/a

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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C232-E05: attB2r-meGFP-attB3

Plasmid

#162902

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal eGFP fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric enhanced Green fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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pJDC181

Plasmid

#60853

Purpose

Expresses click beetle red luciferase

Depositor

Jeffrey Cirillo

Article

Chang et al PLoS One. 2014 Sep 29;9(9):e108341. doi:

Insert

Click beetle red luciferase (optimized for Mycobacteria)

Use

Tags

Expression

Bacterial

Mutation

codon optimized for Mycobacteria

Promoter

hsp60

Available since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pCF CREB

Plasmid

#22968

Depositor

Marc Montminy

Article

Du et al Mol Cell Biol. 2000 Jun . 20(12):4320-7.

Insert

CREB (Creb1 Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pECNUS_MP02

Plasmid

#194351

Purpose

CRISPR-Cas9 multiplexing acceptor clone with BsaI cutter

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

pcoCas9

Use

Tags

Expression

Plant

Mutation

Promoter

RPS5A

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pEM1 = flp-21::LoxPStopLoxP::npr-1 SL2 GFP

Plasmid

#24033

Depositor

Cori Bargmann

Article

Macosko et al Nature. 2009 Apr 30. 458(7242):1171-5.

Insert

Npr1 (npr-1 Nematode)

Use

Cre/Lox

Tags

GFP

Expression

Worm

Mutation

Promoter

Available since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-4T-3-SpSuc1

Plasmid

#162558

Purpose

Expresses GST-tagged S.pombe Suc1 in E.coli

Depositor

Iain Hagan

Article

Singh et al Methods Mol Biol. 2021;2329:123-142. doi

Insert

Schizosaccharomyces pombe Suc1 (suc1 Fission Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-ChETA-EYFP

Plasmid

#26967

Purpose

3rd gen lentiviral expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

Insert

hChR2(E123T/H134R)-EYFP

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

E123T

Promoter

CaMKIIa

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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PlayBack-CMV-Kpn1

Plasmid

#203302

Purpose

For expression of eukaryotic genes of interest with CMV promoter with Kpn1 as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pH_35S/mEGFP

Plasmid

#135321

Purpose

mEGFP expression regulated by the CaMV35S promoter, co-expressed hygromycin phosphotransferase selectable marker (hpt)

Depositor

Mathias Pribil

Article

Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

Insert

mEGFP

Use

Tags

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S

Available since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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vYM112_PB-pEF1a-optiPIN2-HA(Hygro)

Plasmid

#160045

Purpose

For expressing the active auxin pump PIN2

Depositor

Michael Elowitz

Article

Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

Insert

PIN2

Use

Synthetic Biology

Tags

HA

Expression

Mammalian

Mutation

codon optimized

Promoter

pEF1s

Available since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTR47m4

Plasmid

#104068

Purpose

Luciferase Reporter for Formaldehyde Sensing

Depositor

David Liu

Article

Woolston et al Biotechnol Bioeng. 2017 Sep 16. doi: 10.

Inserts

xluxAB
frmR
frm Promoter m4

Use

Luciferase and Synthetic Biology

Tags

Expression

Bacterial

Mutation

23r and 25th nucleotides in sequence mutated from…

Promoter

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pNICE-LAP-neurexin-1beta

Plasmid

#42575

Depositor

Alice Ting

Article

Liu et al ACS Nano. 2012 Dec 21;6(12):11080-7. doi

Insert

LAP-neurexin-1beta

Use

Tags

c-Myc, after signal peptide

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCLE NICD3

Plasmid

#26894

Depositor

Nicholas Gaiano

Article

Dang et al Dev Neurosci. 2006;28(1-2):58-69.

Insert

NICD3 (NOTCH3 Human)

Use

Retroviral

Tags

Alkaline Phosphatase and IRES

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pDR FLIP43 GPS1

Plasmid

#101084

Purpose

GA biosensor expression plasmid

Depositor

Wolf Frommer, Alexander Jones

Article

Rizza et al Nat Plants. 2017 Oct 2. doi: 10.1038/s41

Insert

GPS1

Use

Tags

6HIS and cMyc

Expression

Yeast

Mutation

Promoter

pPMA1

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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p70a-T7RNAP

Plasmid

#105683

Purpose

Expresses T7 polymerase in cell-free TXTL.

Depositor

Chase Beisel

Article

Maxwell et al Methods. 2018 Jul 1;143:48-57. doi: 10.1

Insert

T7 polymerase

Use

Tags

Expression

Bacterial

Mutation

Promoter

OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

Available since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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