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pBAD-MtrxRFP2

Plasmid

#172323

Purpose

fluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)

Depositor

Huiwang Ai

Article

Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.

Insert

human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pJCC_084 Cas12i1 D647A

Plasmid

#171671

Purpose

For bacterial expression of Cas12i1 D647A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

Cas12i1 D647A

Use

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed aspartate 647 to alanine

Promoter

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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p415 Glo3 S389,398 A

Plasmid

#129485

Purpose

Expression in S. cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 S389,398 A (GLO3 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Mutation of serines 389 and 398 to alanine

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Glo3 S389,398 D

Plasmid

#129486

Purpose

Expression in S. cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 S389,398 D (GLO3 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Mutation of serines 389 and 398 to Asp

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Met25 Glo3 ΔGRM-ΔC

Plasmid

#129487

Purpose

Expression in S. cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 ΔGRM ΔC (GLO3 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Δ376-493 (deletion of Glo3 motif & C-terminal…

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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A102-GAP-BoCCS

Plasmid

#123287

Purpose

Expression of truncated Glo3 (1-375) in E.Coli

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 (GLO3 Budding Yeast)

Use

Tags

His and MBP

Expression

Bacterial

Mutation

1-375

Promoter

T5

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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A102-BoCCS-GRM

Plasmid

#123288

Purpose

Expression of truncated Glo3 (137-459) in E.Coli

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 (GLO3 Budding Yeast)

Use

Tags

His and MBP

Expression

Bacterial

Mutation

137-459

Promoter

T5

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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A102-Par-BoCCS-GRM

Plasmid

#123289

Purpose

Expression of truncated Glo3 (296-459) in E.Coli

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 (GLO3 Budding Yeast)

Use

Tags

His and MBP

Expression

Bacterial

Mutation

296-459

Promoter

T5

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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A102-BoCCS

Plasmid

#123290

Purpose

Expression of truncated Glo3 (137-375) in E.Coli

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 (GLO3 Budding Yeast)

Use

Tags

His and MBP

Expression

Bacterial

Mutation

137-375

Promoter

T5

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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A102-GRM

Plasmid

#123291

Purpose

Expression of truncated Glo3 (373-459) in E.Coli

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 (GLO3 Budding Yeast)

Use

Tags

His and MBP

Expression

Bacterial

Mutation

373-459

Promoter

T5

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Glo3 R59K ΔC

Plasmid

#112653

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 R59K ΔC

Use

Tags

Expression

Yeast

Mutation

R59K, Δ431-493 (deletion of C-terminal amphipathi…

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Glo3 R59K ΔGRM-ΔC

Plasmid

#112654

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 R59K ΔGRM ΔC

Use

Tags

Expression

Yeast

Mutation

R59K, Δ376-493 (deletion of Glo3 motif & C-te…

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Glo3 R59K S389,398 A

Plasmid

#112656

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 R59K S389,398A

Use

Tags

Expression

Yeast

Mutation

R59K, mutations (S389A, S398A) in Glo3 motif, non…

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Glo3 R59K S389,398 D

Plasmid

#112657

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 R59K S389,398D

Use

Tags

Expression

Yeast

Mutation

R59K, mutations (S389D, S398D) in Glo3 motif, pho…

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 GFP-Glo3 ΔGRM-ΔC

Plasmid

#112659

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

GFP-Glo3 ΔGRM ΔC

Use

Tags

sfGFP

Expression

Yeast

Mutation

Δ376-493 (deletion of Glo3 motif & C-terminal…

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 GFP-Glo3 S389,398 A

Plasmid

#112660

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

GFP- Glo3 S389,398A

Use

Tags

sfGFP

Expression

Yeast

Mutation

mutations (S389A, S398A) in Glo3 motif, non-phosp…

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 GFP-Glo3 S389,398 D

Plasmid

#112661

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

GFP-Glo3 S389,398D

Use

Tags

sfGFP

Expression

Yeast

Mutation

mutations (S389D, S398D) in Glo3 motif, phosphomi…

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Gcs1 R54K L246D

Plasmid

#112648

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Gcs1 R54K L246D

Use

Tags

Expression

Yeast

Mutation

R54K & L246D (in ALPS motif)

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Gcs1 R54K AxxA

Plasmid

#112649

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Gcs1 R54K AxxA

Use

Tags

Expression

Yeast

Mutation

R54K & Alanine substitution of C-terminal δL …

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Gcs1 Δ3xF AxxA

Plasmid

#112650

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Gcs1 Δ3xF AxxA

Use

Tags

Expression

Yeast

Mutation

R54K, W304A, W307A, W310A, W349A, F353A

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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