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pMVS142_pACT1_mCherry_Zif268_EBD_MCS_KAN

Plasmid

#99049

Purpose

Transcription Factor chassis for activation domains. mCherry, Zif268 DNA binding domain, estrogen response domain

Depositor

Barak Cohen

Article

Staller et al Cell Syst. 2018 Mar 1. pii: S2405-4712(1

Inserts

ACT1 promoter (ACT1 Budding Yeast)
mCherry
Zif268 DNA binding Domain
Estrogen response domain (ESR1 Human)

Expression

Yeast

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-KIF1B beta-FL

Plasmid

#79108

Purpose

Mammalian expression of GFP-KIF1B beta-FL

Depositor

Susanne Schlisio

Article

Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi

Insert

GFP-KIF1B beta full length (KIF1B Human)

Tags

GFP

Expression

Mammalian

Mutation

exon 25 missing (natural splice varient)

Promoter

CMV

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only
pLVX MLKL(S345D)-2A-mCherry-puro

Plasmid

#231975

Purpose

Tet inducible expression of DmrB-Mlkl with mCherry to induce phosphomimetic MLKL S345D necroptosis

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

MLKL S345D construct with bi-cistronic mCherry (Mlkl Mouse)

Use

Lentiviral

Mutation

MLKL S345D

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-2dV-Camui (wt)

Plasmid

#220366

Purpose

Improved FLIM sensor for CaMK2-alpha activation (mammalian expression, cytosol)

Depositor

Ryohei Yasuda

Article

Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

Insert

mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(wt)-mEGFP(A206K) (Camk2a Rat, Synthetic)

Tags

mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCROPseq-VEX-optimized-scaffold

Plasmid

#203321

Purpose

Modified CROPseq vector for efficient CRISPR screens in hematopoiesis

Depositor

Vijay Sankaran

Article

Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

Insert

sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pSBbi-NLSmCherryNLS Clover-HDHB

Plasmid

#190223

Purpose

Fluorescent cell cycle reporter

Depositor

Laura Heiser

Article

Gross et al Nat Commun. 2023 Jun 10;14(1):3450. doi:

Inserts

Human DNA Helicase B (amino acids 994-1087) (HELB Human)
NLS-mCherry-NLS-P2A-Puromycin

Use

Transposon

Tags

Clover fluorescent protein

Expression

Mammalian

Mutation

Amino Acids 994-1087

Promoter

EF1a and RPBSA

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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LCV2 STING KO

Plasmid

#217445

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human STING

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

sgRNA targeting STING (STING1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX MLKL(1-180)-2A-mCherry-puro

Plasmid

#231976

Purpose

Tet inducible expression of DmrB-Mlkl with mCherry to induce MLKL 1-180 necroptosis

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

MLKL 1-180 construct with bi-cistronic mCherry (Mlkl Mouse)

Use

Lentiviral

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2 puro - CRBN (#1)

Plasmid

#166240

Purpose

sgRNA (#1) to generate a knockout of human CRBN by targeting the middle region of Exon1

Depositor

Günter Schneider

Article

Lier et al Bioorg Chem. 2021 Nov 20:105505. doi: 10

Insert

CRBN (CRBN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 20, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2 puro - CRBN (#2)

Plasmid

#166241

Purpose

sgRNA (#2) to generate a knockout of human CRBN by targeting the start region of Exon1

Depositor

Günter Schneider

Article

Lier et al Bioorg Chem. 2021 Nov 20:105505. doi: 10

Insert

CRBN (CRBN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 20, 2021

Availability

Academic Institutions and Nonprofits only

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