We narrowed to 16,802 results for: URE

1. Seattle_IPDA_control_14_004

   Plasmid

   #167350

   Purpose

   ddPCR gating control for droplets containing the env target

   Depositor

   Florian Hladik

   Article

   Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

   Insert

   env
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Seattle_IPDA_control_32_007

   Plasmid

   #167353

   Purpose

   ddPCR gating control for droplets containing 5'pol or 3'pol targets

   Depositor

   Florian Hladik

   Article

   Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

   Insert

   pol
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Seattle_IPDA_control_17_005

   Plasmid

   #167351

   Purpose

   ddPCR gating control for droplets containing gat and tat targets

   Depositor

   Florian Hladik

   Article

   Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

   Insert

   gat_tat
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJSC173 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant

   Plasmid

   #101218

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant N692A/M694A/Q695A/H698A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   N692A, M694A, Q695A and H698A

   Promoter

   T7

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST-FRT/T0-Flag-BRCA1

   Plasmid

   #71117

   Purpose

   mammalian expression of Flag-BRCA1 siRNA resistant construct

   Depositor

   Daniel Durocher

   Article

   Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

   Insert

   BRCA1 (BRCA1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TO

   Available since

   Nov. 12, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. (364) SB KRAB-SadCas9

   Plasmid

   #163022

   Purpose

   Sleeping Beauty TET-On expression of CRISPRi (without U6-gRNA)

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Mammalian codon-optimized SadCas9
   

   Use

   Transposon

   Tags

   KRAB and myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   TET-On

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSP1101

   Plasmid

   #65773

   Purpose

   Human expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335E, and T1337R mutations in C…

   Promoter

   CMV & T7

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSP2133

   Plasmid

   #72249

   Purpose

   Human expression plasmid for SpCas9-HF4 variant: CMV-T7-humanSpCas9-HF4(Y450A, N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 HF4(Y450A/N497A/R661A/Q695A/Q926A)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   Y450A, N497A, R661A, Q695A, and Q926A

   Promoter

   CMV

   Available since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. TDP-43_CTD_del321-343

   Plasmid

   #98676

   Purpose

   Expresses 6xHis-tagged C-terminal domain of TDP-43 with a deletion of AA 321-343

   Depositor

   Nicolas Fawzi

   Article

   Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   del321-343

   Promoter

   Available since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiCas9-Venus

    Plasmid

    #70267

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 protein and Venus reporter from EFS promoter. Lentiviral backbone.

    Depositor

    Daniel Bauer

    Article

    Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature

    Inserts

    Cas9
    Venus Reporter
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS and P2A

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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