We narrowed to 8,862 results for: BLI;
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Plasmid#51137PurposeExpress turbo GFP tagged three SH3 domains of Cin85 (truncation) in mammalian cellsDepositorInsertCin85 (SH3KBP1 Human)
UseTagsTurboGFPExpressionMammalianMutationcontains 3 SH3 domainsPromoterCMVAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HIS-TagGFP2-TEV-GGG-ELP(120nm)-Cys
Plasmid#90473PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Term TagGFP2, and a TEV-cleavage site, leaving an N-terminal Sortase Recognition Sequence GGG and a C-terminal CysteineDepositorInsertTagGFP2-TEV-GGG-ELP(120nm)-Cys
UseTagsCysteine and Sortase-TagExpressionBacterialMutationPromoterT7Available sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ACE2
Plasmid#158438PurposeGateway Cloning compatible entry vector for the human ACE2 gene.DepositorInsertACE2 (ACE2 Human)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTol2-ziUAS:GPHN.FingR-mCherry-ZFI (IL2RGTC)-KRAB(A)
Plasmid#72639PurposeTol2 based UAS modified with a IL2RGTC binding site upstream of it, driving GPHN-FingR fused to mCherry, IL2RGTC zinc finger, and KRAB(A) repressor domainDepositorInsertUAS upstream of GPHN-FingR with ZFI(IL2RFTC) and KRAB(A)
UseGatewayTagsExpressionMutationStop codon after mCherry- SEE DEPOSITOR COMMENTS …PromoterAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC-T7-J1~398-Luc
Plasmid#208639Purposefor in vitro transcription template preparation, insert T7 promoter, JFH1(AB047639)_1~398 and F-luc from pGL3-Basic, into pUC19DepositorInsertHepatitis C virus (HCV) strain JFH1(AB047639) nt 1~398
UseLuciferase; In vitro transcription templateTagsFirefly Luciferase (F-Luc and T7 promoterExpressionMutationnt 1~398, in frame with donstream F-LucPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pFRT-TODestFLAGHA_HuC
Plasmid#65756Purposeexpresses FLAGHA-tagged HuC in mammalian cellsDepositorInsertELAVL3 (ELAVL3 Human)
UseTagsFLAG HA and V5ExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
CD55-full length
Plasmid#220874PurposeExpresses CD55 variant in mammalian cellsDepositorInsertCD55 (CD55 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMWAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
TH mAb_BCN53.1.1D10_HC_Mouse IgG2a
Plasmid#182896PurposeMammalian expression of TH mouse mAb HC geneDepositorInsertanti-TH (human) heavy chain (TH Mouse)
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationNoPromoterCMVAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-On-ARNTL-shRNA3
Plasmid#166915PurposeLentivirus for inducible knockdown of human ARNTL(BMAL1)DepositorInsertARNTL shRNA (BMAL1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro
Plasmid#69546PurposeTet-On system expressing MyoD-2A-GFP-2xNLS.DepositorInsertsUseLentiviralTags2x nuclear localization sequence and T2A "sk…ExpressionMutationRemoved stop codon to fuse it to the 2A-eGFPnls i…PromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
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XLone-Puro NFIL3 P2A eGFP NLS
Plasmid#140026PurposeTunable and temporal expression control of NFIL3 and nuclear eGFPDepositorInsertNFIL3 and enhanced Green Fluorescent Protein (NFIL3 Synthetic, Human)
UseSynthetic BiologyTagseGFPExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNY-345
Plasmid#231045PurposeUsed for staining GFPDepositorInsertanti GFP DARPin fused to rabbit Fc
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNY-347
Plasmid#231047PurposeUsed for GFP stainingDepositorInsertanti GFP DARPin fused to mouse Fc
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestGFP_HuC
Plasmid#65762Purposeexpresses GFP-tagged HuC in mammalian cellsDepositorInsertELAVL3 (ELAVL3 Human)
UseTagsFLAG HA and V5ExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-ACE2-G418
Plasmid#158450PurposeConstitutive lentiviral expression of human ACE2.DepositorInsertACE2 (ACE2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
KRigor980
Plasmid#129763PurposeExpresses full-length Drosophila Kinesin-1 with rigor mutation (R210A) for adhering microtubules to surfacesDepositorInsertKHC (Khc Fly)
UseTagsHis6ExpressionBacterialMutationR210APromoterAvailable sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
KIF3A406
Plasmid#129768PurposeExpresses mouse Kinesin-2 KIF3A head and neck linker, dimerized through kinesin-1 neck-coil to 406 for biochemical assaysDepositorInsertKIF3A (Kif3a Mouse)
UseTagsHis6ExpressionBacterialMutationTruncation at 359, fused to kinesin-1 starting Al…PromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCENP
Plasmid#39185PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertINCENP (INCENP Human)
UseTagsGST-TevExpressionBacterialMutationComplex with AURKBA-c004PromoterAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-DEST-FLAG
Plasmid#119754PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal FLAG tag.DepositorTypeEmpty backboneUseTagsFLAG and GSTExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
KIF3A560GFP
Plasmid#129769PurposeExpresses mouse Kinesin-2 KIF3A head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP taggedDepositorInsertKIF3A (Kif3a Mouse)
UseTagsGFP and His6ExpressionBacterialMutationTruncation at 359, fused to kinesin-1 starting Al…PromoterAvailable sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
RIX-F2Oct4F3-GFP
Plasmid#216848PurposeThis plasmid is for the production of lentiviral vectors that can be used to track pluripotent stem cells, such as ES cells, iPS and tissue-resident pluripotent cells. GFP expression is lost upon diffDepositorInsertGFP
UseLentiviralTagsExpressionMutationPromoterOct4Available sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA_DiCre2_60.F2
Plasmid#60210PurposeExpresses a fusion protein of FKBP12(F36V)-linker peptide 2- Cre(60-343)DepositorInsertCre recombinase (CD207 Synthetic, Bacteriophage P1, Human)
UseTagsFKBP12(F36V) and Nuclear localisation signalExpressionMammalianMutationFKBP12 has the F36V mutation; amino acids 60-343 …PromoterCMVAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pKG96
Plasmid#63072PurposeRetroviral expression of EGFP-TEV-S-tag CENP-A for protein localization and affinity purification (LAP)DepositorInsertCENP-A (CENPA Human)
UseRetroviralTagsEGFP and TEV-SExpressionMammalianMutationA12V, C75Y, and L121F mutations compared to GenBa…PromoterAvailable sinceJan. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-FLEX-mRuby3-WPRE
Plasmid#99279PurposeCan be used to generate AAV virus that will express mRuby3 in the presence of CreDepositorInsertmRuby3
UseAAV and Cre/LoxTagsExpressionMutationPromoterAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
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K560_AviC_coBirA
Plasmid#129765PurposeExpresses Drosophila Kinesin-1 truncated at 560 and biotinylated at tail for single-molecule assaysDepositorInsertKHC (Khc Fly)
UseTagsAvi-tag GLNDIFEAQKIEWH and His6ExpressionBacterialMutationTruncation at 559PromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
p2Bac.His.XPB
Plasmid#39216DepositorInsertXPB (ERCC3 Human)
UseTagsHisExpressionInsectMutationPromoterPphAvailable sinceAug. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
p2Bac.His.XPD
Plasmid#39217DepositorInsertXPD (ERCC2 Human)
UseTagsHis (HHYHHH)ExpressionInsectMutationXPD starts at aa4PromoterPphAvailable sinceAug. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
TH mAb_BCN53.1.1D10_HC_Rat IgG2a
Plasmid#182898PurposeMammalian expression of TH rat mAb HC geneDepositorInsertanti-TH (human) light chain (TH Rat)
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationNoPromoterCMVAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TH mAb_BCN53.1.1D10_LC_Rat Ig Kappa
Plasmid#182899PurposeMammalian expression of TH rat mAb LC geneDepositorInsertanti-TH (human) light chain (TH Rat)
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationNoPromoterCMVAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
psd44-G13WT
Plasmid#46829Purposeexpression of wild type G protein alpha 13 in mammalian cellsDepositorInsertGNA13 (GNA13 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterPGK promoterAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pLenti-EF1α-IRES-EGFP-hMCIDAS
Plasmid#149696PurposeExpresses untagged human MCIDAS in mammalian cellsDepositorInsertMCIDAS (MCIDAS Human)
UseLentiviralTagsExpressionMutationPromoterEF1 alphaAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5X-GLP-1
Plasmid#66997PurposeExpresses malE-GLP-1 in E. coliDepositorInsertmalE-GLP-1
UseTagsHis6 and malE-Factor XaExpressionBacterialMutationPromotertacAvailable sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLPC-C-SF-TAP-DEST-TLK1-WT
Plasmid#218829PurposeExpresses C-terminal Strep-FLAG-tagged wild type human TLK1 variant 5 in a retrovirus vector.DepositorInsertTLK1 (TLK1 Human)
UseTagsFLAG-STREPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-6His-SUMO-Nsp14
Plasmid#154956PurposeExpresses Nsp14 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coliDepositorInsert6His-SUMO-Nsp14 (ORF1ab SARS-CoV-2)
UseTags6His-Thrombin-SUMOExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
TH mAb_BCN53.1.1D10_LC_Mouse Ig Kappa
Plasmid#182897PurposeMammalian expression of TH mouse mAb LC geneDepositorInsertanti-TH (human) light chain (TH Mouse)
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationNoPromoterCMVAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBJ-FLAG-hPar4
Plasmid#53231Purposemammalian expression of Flag-tagged human Par4DepositorInsertPar4 (F2RL3 Human)
UseTagsFLAG and bovine prolactin signal sequence (MDSKGS…ExpressionMammalianMutationDeletion of aa1-24 (native signal peptide)PromoterAvailable sinceAug. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
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4LEC
Plasmid#60091PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMETTL21A:JMC072-B07:C227766 (METTL21A Human)
UseTags6xHisExpressionBacterialMutationSee commentsPromoterT7Available sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pFRT-DestFLAGHA_HuC
Plasmid#65759Purposeconstitutively expresses FLAG-HA-tagged HuC in mammalian cellsDepositorInsertELAVL3 (ELAVL3 Human)
UseTagsFLAG HAExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pAllet
Plasmid#202560PurposeE. coli cloning vector with large MCS (50+ unique restriction sites)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only