We narrowed to 4,495 results for: Ado

1. pLPC-puromycin-binary-MDH1-3xFLAG-ME1- HA

   Plasmid

   #184549

   Purpose

   Retroviral vector to co-express human MDH1 with 3xFLAG tag and human ME1 with HA tag

   Depositor

   Gerardo Ferbeyre

   Article

   Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

   Inserts

   Malate dehydogenase 1 (MDH1 Human)
   Malic Enzyme 1 (ME1 Human)
   

   Use

   Retroviral

   Tags

   3xFLAG and HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUltra-MDH1-ME1

   Plasmid

   #184465

   Purpose

   Lentiviral vector for tri-cistronic expression of EGFP, MDH1 and ME1 (seperated by P2A and T2A)

   Depositor

   Gerardo Ferbeyre

   Article

   Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

   Inserts

   Malate dehydrogenase 1 (MDH1 Human)
   Malic Enzyme 1 (ME1 Human)
   

   Use

   Lentiviral

   Tags

   fused to T2A

   Expression

   Mammalian

   Mutation

   deletion of stop codon

   Promoter

   UbC

   Available Since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiSAMv2 OPTN TSS-guide4

   Plasmid

   #118199

   Purpose

   CRISPR-mediated activation of OPTN. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   dCas9-VP64-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available Since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. lentiSAMv2 EGFP-guide1

   Plasmid

   #118166

   Purpose

   CRISPRa negative control. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR, and sgRNA1 against the EGFP CDS

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   dCas9-VP64-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available Since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. lentiSAMv2 ZBED3-TSS-guide1

   Plasmid

   #125506

   Purpose

   CRISPR-mediated activation of ZBED3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   dCas9-VP64-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available Since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. lentiSAMv2 CRY2-TSS-guide3

   Plasmid

   #125491

   Purpose

   CRISPR-mediated activation of CRY2. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   dCas9-VP64-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available Since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. lentiSAMv2 CRY2-TSS-guide2

   Plasmid

   #125490

   Purpose

   CRISPR-mediated activation of CRY2. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   dCas9-VP64-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available Since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. lentiSAMv2 TCF7L2 TSS-guide1

   Plasmid

   #118184

   Purpose

   CRISPR-mediated activation of TCF7L2. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   dCas9-VP64-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available Since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. lentiSAMv2 TCF7L2 TSS-guide4

   Plasmid

   #118187

   Purpose

   CRISPR-mediated activation of TCF7L2. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   dCas9-VP64-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available Since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. lentiSAMv2 TCF7L2 TSS-guide3

    Plasmid

    #118186

    Purpose

    CRISPR-mediated activation of TCF7L2. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available Since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. lentiSAMv2 EGFP-guide2

    Plasmid

    #118167

    Purpose

    CRISPRa negative control. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR, and sgRNA2 against the EGFP CDS

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. lentiSAMv2 C2CD4B-TSS-guide4

    Plasmid

    #125485

    Purpose

    CRISPR-mediated activation of C2CD4B. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. lentiSAMv2 C2CD4A-TSS-guide2

    Plasmid

    #125481

    Purpose

    CRISPR-mediated activation of C2CD4A. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. lentiSAMv2 VPS13C-TSS-guide2

    Plasmid

    #125477

    Purpose

    CRISPR-mediated activation of VPS13C. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. lentiSAMv2 C2CD4A/B-enh3-guide3

    Plasmid

    #125475

    Purpose

    CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs17205526 (C2CD4A/B GWAS locus)

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. lentiSAMv2 C2CD4A/B-enh2-guide2

    Plasmid

    #125470

    Purpose

    CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs7163757 (C2CD4A/B GWAS locus)

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. lentiSAMv2 C2CD4A/B-enh1-guide3

    Plasmid

    #125467

    Purpose

    CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs182222907 (C2CD4A/B GWAS locus)

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET21b(+)_SARS-CoV-2_3CLpro_C145A-HIS

    Plasmid

    #224698

    Purpose

    Bacterial Expression and Purification of SARS-CoV-2 3CLpro inactive protease with His-tag

    Depositor

    Christopher Overall

    Article

    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

    Insert

    SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    

    Use

    Tags

    Gly-Gly-6xHis

    Expression

    Bacterial

    Mutation

    silent mutation C to T at position 10546 (elimina…

    Promoter

    T7

    Available Since

    July 2, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. lentiSAMv2 rs58692659-guide4

    Plasmid

    #125512

    Purpose

    CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs58692659 (ZFAND3 GWAS locus)

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. lentiSAMv2 rs58692659-guide3

    Plasmid

    #125511

    Purpose

    CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs58692659 (ZFAND3 GWAS locus)

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge